pLenti-hTERT-Neo

                            FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     primer_bind     5942..5958
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             6035..6061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      6261..6396
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6491..7285
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7298..7885
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7769..7780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    7895..8128
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             7948..8128
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8200..8321
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             9650..10444
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca
     1801 aaattttcgg gtttttcgaa cctagggttc cgcgttacat aacttacggt aaatggcccg
     1861 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1921 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1981 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2041 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2101 cagtacatct acgtttagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     2161 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2221 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     2281 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2341 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
     2401 gaaccgagtt taaactccct atcagtgata gagatctccc tatcagtgat agagagctag
     2461 ccccgggatc gatcaattga gtacttacgt aggtacccca gtgtggtggc ctgcaggtga
     2521 attccaccat gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact
     2581 accgcgaggt gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc
     2641 tggtgcagcg cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg
     2701 tgccctggga cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga
     2761 aggagctggt ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg
     2821 ccttcggctt cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca
     2881 gcgtgcgcag ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg
     2941 ggctgctgct gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc
     3001 tctttgtgct ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc
     3061 tcggcgctgc cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg
     3121 gatgcgaacg ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag
     3181 ccccgggtgc gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc
     3241 ccaggcgtgg cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc
     3301 acccgggcag gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac
     3361 ccgccgaaga agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat
     3421 ccgtgggccg ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg
     3481 acacgccttg tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca
     3541 aggagcagct gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc
     3601 ggaggctcgt ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca
     3661 ggttgccccg cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg
     3721 ggaaccacgc gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg
     3781 cggtcacccc agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc
     3841 ccgaggagga ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc
     3901 cctggcaggt gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct
     3961 ggggctccag gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg
     4021 ggaagcatgc caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cgggactgcg
     4081 cttggctgcg caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg
     4141 aggagatcct ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca
     4201 ggtctttctt ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga
     4261 agagtgtctg gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc
     4321 tgcgggagct gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga
     4381 cgtccagact ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact
     4441 acgtcgtggg agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg
     4501 tgaaggcact gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg
     4561 cctctgtgct gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc
     4621 gggcccagga cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg
     4681 acaccatccc ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca
     4741 cgtactgcgt gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg
     4801 ccttcaagag ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg
     4861 ctcacctgca ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc
     4921 tgaatgaggc cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg
     4981 tgcgcatcag gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct
     5041 ccacgctgct ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc
     5101 ggcgggacgg gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca
     5161 cccacgcgaa aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg
     5221 tgaacttgcg gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg
     5281 cttttgttca gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc
     5341 ggaccctgga ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc
     5401 tcaccttcaa ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct
     5461 tgcggctgaa gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt
     5521 gcaccaacat ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc
     5581 agctcccatt tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg
     5641 acacggcctc cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg
     5701 ccaagggcgc cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat
     5761 tcctgctcaa gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga
     5821 cagcccagac gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg
     5881 cagccaaccc ggcactgccc tcagacttca agaccatcct ggactgatgg gacgcggccg
     5941 ctctagaact agtggatccc ccgggctgca ggaattcact agtaccggta ggcctgtcga
     6001 cgatatcggg cccgcggccg ctggatcctc tagataccca tacgacgtcc cagactacgc
     6061 ttgatccccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6361 tattccagaa gtagtgagga ggcttttttg gaggcctagt gaccagatct aatgcgtgct
     6421 agttttgcaa aaagcttggg ctgcaggtcg aggcggatct gatcaagaga caggatgagg
     6481 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6541 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6601 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6661 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6721 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6781 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6841 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6901 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6961 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     7021 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7081 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     7141 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     7201 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     7261 tcgccttctt gacgagttct tctgaacgcg ttccggaaat caacctctgg attacaaaat
     7321 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7381 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7441 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7501 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7561 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7621 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7681 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7741 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7801 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg
     7861 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga
     7921 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7981 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8041 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8101 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     8161 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     8221 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     8281 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt
     8341 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga
     8401 atcggccaac ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8461 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8521 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8581 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8641 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8701 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8761 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8821 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8881 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8941 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9001 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9061 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9121 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9181 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9241 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9301 gattttggcc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc
     9361 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     9421 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     9481 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     9541 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     9601 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     9661 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     9721 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     9781 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     9841 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     9901 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     9961 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    10021 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    10081 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    10141 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
    10201 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    10261 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    10321 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    10381 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    10441 ctgaattttg ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10501 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    10561 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    10621 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    10681 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    10741 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    10801 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    10861 tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata
    10921 atataccttt tttggattga agccaatatg ataatgaggg ggtggagttt gtgacgtggc
    10981 gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc ggaagtgtga tgttgcaagt
    11041 gtggcggaac acatgtaagc gacggatgtg gcaaaagtga cgtttttggt gtgcgccggt
    11101 gtacacagga agtgacaatt ttcgcgcggt tttaggcgga tgttgtagta aatttgggcg
    11161 taaccgagta agatttggcc attttcgcgg gaaaactgaa taagaggaag tgaaatctga
    11221 ataattttgt gttactcata gcgcgtaata ctctcatgag cggatacata tttgaatgta
    11281 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    11341 taactataac ggtcctaagg tagcgaaaat gtagtcttat gcaatactct tgtagtcttg
    11401 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
    11461 ccgattggtg gaagtaaggt ggtacgat
                        

                            FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             109..289
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    336..461
                     /note="HIV-1 psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    951..1184
                     /note="HIV-1 RRE"
                     /note="The Rev response element (RRE) of human 
                     immunodeficiency virus type 1 allows for Rev-dependent mRNA
                     export from the nucleus to the cytoplasm."
                     /note="color: #ffe4c4"
     enhancer        1831..2135
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2136..2339
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    2416..2434
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     protein_bind    2437..2455
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tet operator"
                     /note="
                     "
                     /note="color: #31849b"
     primer_bind     5942..5958
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     CDS             6035..6061
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     rep_origin      6261..6396
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             6491..7285
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    7298..7885
                     /note="WPRE"
                     /note="woodchuck posttranscriptional regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7769..7780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /note="color: #cc99b2"
                     /translation="IEGR"
     misc_feature    7895..8128
                     /note="3'LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     LTR             7948..8128
                     /note="5' LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    8200..8321
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(8650..9238)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             9650..10444
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"