pLenti-hTERT-No-Selection
| Vector Name | pLenti-hTERT-No-Selection |
| VectorType | Lentiviral plasmid |
| Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression is driven by the CMV promoter, without any Tag or reporter. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg
cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag
tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg
tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc
gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg
aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag
catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg
tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag
gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg
caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac
aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact
tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg
ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc
agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt
ggtggcctgc
3121 aggtgaattc caccatgccg cgcgctcccc gctgccgagc cgtgcgctcc
ctgctgcgca
3181 gccactaccg cgaggtgctg ccgctggcca cgttcgtgcg gcgcctgggg
ccccagggct
3241 ggcggctggt gcagcgcggg gacccggcgg ctttccgcgc gctggtggcc
cagtgcctgg
3301 tgtgcgtgcc ctgggacgca cggccgcccc ccgccgcccc ctccttccgc
caggtgtcct
3361 gcctgaagga gctggtggcc cgagtgctgc agaggctgtg cgagcgcggc
gcgaagaacg
3421 tgctggcctt cggcttcgcg ctgctggacg gggcccgcgg gggccccccc
gaggccttca
3481 ccaccagcgt gcgcagctac ctgcccaaca cggtgaccga cgcactgcgg
gggagcgggg
3541 cgtgggggct gctgctgcgc cgcgtgggcg acgacgtgct ggttcacctg
ctggcacgct
3601 gcgcgctctt tgtgctggtg gctcccagct gcgcctacca ggtgtgcggg
ccgccgctgt
3661 accagctcgg cgctgccact caggcccggc ccccgccaca cgctagtgga
ccccgaaggc
3721 gtctgggatg cgaacgggcc tggaaccata gcgtcaggga ggccggggtc
cccctgggcc
3781 tgccagcccc gggtgcgagg aggcgcgggg gcagtgccag ccgaagtctg
ccgttgccca
3841 agaggcccag gcgtggcgct gcccctgagc cggagcggac gcccgttggg
caggggtcct
3901 gggcccaccc gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg
gtgtcacctg
3961 ccagacccgc cgaagaagcc acctctttgg agggtgcgct ctctggcacg
cgccactccc
4021 acccatccgt gggccgccag caccacgcgg gccccccatc cacatcgcgg
ccaccacgtc
4081 cctgggacac gccttgtccc ccggtgtacg ccgagaccaa gcacttcctc
tactcctcag
4141 gcgacaagga gcagctgcgg ccctccttcc tactcagctc tctgaggccc
agcctgactg
4201 gcgctcggag gctcgtggag accatctttc tgggttccag gccctggatg
ccagggactc
4261 cccgcaggtt gccccgcctg ccccagcgct actggcaaat gcggcccctg
tttctggagc
4321 tgcttgggaa ccacgcgcag tgcccctacg gggtgctcct caagacgcac
tgcccgctgc
4381 gagctgcggt caccccagca gccggtgtct gtgcccggga gaagccccag
ggctctgtgg
4441 cggcccccga ggaggaggac acagaccccc gtcgcctggt gcagctgctc
cgccagcaca
4501 gcagcccctg gcaggtgtac ggcttcgtgc gggcctgcct gcgccggctg
gtgcccccag
4561 gcctctgggg ctccaggcac aacgaacgcc gcttcctcag gaacaccaag
aagttcatct
4621 ccctggggaa gcatgccaag ctctcgctgc aggagctgac gtggaagatg
agcgtgcggg
4681 actgcgcttg gctgcgcagg agcccagggg ttggctgtgt tccggccgca
gagcaccgtc
4741 tgcgtgagga gatcctggcc aagttcctgc actggctgat gagtgtgtac
gtcgtcgagc
4801 tgctcaggtc tttcttttat gtcacggaga ccacgtttca aaagaacagg
ctctttttct
4861 accggaagag tgtctggagc aagttgcaaa gcattggaat cagacagcac
ttgaagaggg
4921 tgcagctgcg ggagctgtcg gaagcagagg tcaggcagca tcgggaagcc
aggcccgccc
4981 tgctgacgtc cagactccgc ttcatcccca agcctgacgg gctgcggccg
attgtgaaca
5041 tggactacgt cgtgggagcc agaacgttcc gcagagaaaa gagggccgag
cgtctcacct
5101 cgagggtgaa ggcactgttc agcgtgctca actacgagcg ggcgcggcgc
cccggcctcc
5161 tgggcgcctc tgtgctgggc ctggacgata tccacagggc ctggcgcacc
ttcgtgctgc
5221 gtgtgcgggc ccaggacccg ccgcctgagc tgtactttgt caaggtggat
gtgacgggcg
5281 cgtacgacac catcccccag gacaggctca cggaggtcat cgccagcatc
atcaaacccc
5341 agaacacgta ctgcgtgcgt cggtatgccg tggtccagaa ggccgcccat
gggcacgtcc
5401 gcaaggcctt caagagccac gtctctacct tgacagacct ccagccgtac
atgcgacagt
5461 tcgtggctca cctgcaggag accagcccgc tgagggatgc cgtcgtcatc
gagcagagct
5521 cctccctgaa tgaggccagc agtggcctct tcgacgtctt cctacgcttc
atgtgccacc
5581 acgccgtgcg catcaggggc aagtcctacg tccagtgcca ggggatcccg
cagggctcca
5641 tcctctccac gctgctctgc agcctgtgct acggcgacat ggagaacaag
ctgtttgcgg
5701 ggattcggcg ggacgggctg ctcctgcgtt tggtggatga tttcttgttg
gtgacacctc
5761 acctcaccca cgcgaaaacc ttcctcagga ccctggtccg aggtgtccct
gagtatggct
5821 gcgtggtgaa cttgcggaag acagtggtga acttccctgt agaagacgag
gccctgggtg
5881 gcacggcttt tgttcagatg ccggcccacg gcctattccc ctggtgcggc
ctgctgctgg
5941 atacccggac cctggaggtg cagagcgact actccagcta tgcccggacc
tccatcagag
6001 ccagtctcac cttcaaccgc ggcttcaagg ctgggaggaa catgcgtcgc
aaactctttg
6061 gggtcttgcg gctgaagtgt cacagcctgt ttctggattt gcaggtgaac
agcctccaga
6121 cggtgtgcac caacatctac aagatcctcc tgctgcaggc gtacaggttt
cacgcatgtg
6181 tgctgcagct cccatttcat cagcaagttt ggaagaaccc cacatttttc
ctgcgcgtca
6241 tctctgacac ggcctccctc tgctactcca tcctgaaagc caagaacgca
gggatgtcgc
6301 tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg
ctgtgccacc
6361 aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc
ctggggtcac
6421 tcaggacagc ccagacgcag ctgagtcgga agctcccggg gacgacgctg
actgccctgg
6481 aggccgcagc caacccggca ctgccctcag acttcaagac catcctggac
tgatgggacg
6541 cggccgcatg actagttccg gaaatcaacc tctggattac aaaatttgtg
aaagattgac
6601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
taatgccttt
6661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
aatcctggtt
6721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
tgtgcactgt
6781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
tcctttccgg
6841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
gccttgcccg
6901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
cggggaagct
6961 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
ggacgtcctt
7021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
tgctgccggc
7081 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc
cctttgggcc
7141 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca
agatctgctt
7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
ctctggctaa
7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
agtagtgtgt
7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
gtcagtgtgg
7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
acttgcaaag
7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
ttacaaataa
7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
tagttgtggt
7561 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt
gcggagaaag
7621 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg
ccaacgatcc
7681 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
ctcttccgct
7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
atcagctcac
7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
gaacatgtga
7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
gtttttccat
7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
gtggcgaaac
7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
gcgctctcct
8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
aagcgtggcg
8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
ctccaagctg
8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
taactatcgt
8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
tggtaacagg
8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
gcctaactac
8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
taccttcgga
8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
tggttttttt
8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
tttgatcttt
8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
ggtcatgaga
8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
taaatcaatc
8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
tgaggcacct
8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
cgtgtagata
8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
gcgagaccca
8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
cgagcgcaga
8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
ggaagctaga
8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc
ttcacctaga
9001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg
aatgtcagct
9061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta
gcttgcagtg
9121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa
ccggaattgc
9181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg
atggctttct
9241 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca
ggatgaggat
9301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
tgggtggaga
9361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
gccgtgttcc
9421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
ggtgccctga
9481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
gttccttgcg
9541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
ggcgaagtgc
9601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
atcatggctg
9661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
caccaagcga
9721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
caggatgatc
9781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
aaggcgagca
9841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
aatatcatgg
9901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
gcggaccgct
9961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
gaatgggctg
10021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
gccttctatc
10081 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag
ctcatttttt
10141 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac
cgcgataggg
10201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
ctccaacgtc
10261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
acccaaatca
10321 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg
gagcccccga
10381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
gaaagcgaaa
10441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
caccacaccc
10501 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg
aattaattct
10561 taattaacat catcaataat atacctt
LOCUS dna 10587 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3063..3749
/sequence="ORF_6 rf(5)"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
ORF 3135..6533
/sequence="ORF_3 rf(3)"
ORF 4170..5390
/sequence="ORF_5 rf(5)"
misc_binding 5099..5104
/dbxref="REBASE:XhoI"
misc_binding 5187..5192
/dbxref="REBASE:EcoRV"
misc_binding 5623..5628
/dbxref="REBASE:BamHI"
ORF 6533..7171
/sequence="ORF_2 rf(2)"
misc_binding 6540..6547
/dbxref="REBASE:NotI"
Other Gene 7160..7212
/gene="delta_U3 other"
Other Gene 7213..7393
/gene="HIV-1_5_LTR other"
Rep_Origin 7899..8518
/gene="pBR322 origin"
Promoter 9171..9220
/gene="NEOKAN prom"
misc_binding 9278..9283
/dbxref="REBASE:BclI"
ORF 9309..10103
/sequence="ORF_4 rf(3)"
Marker 9312..10100
/gene="NTP_II marker"
Rep_Origin 10208..10513
/gene="f1 origin"
BASE COUNT 2430 a 2812 c 3002 g 2343 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag
1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga
2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag
2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa
2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat
2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag
3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc
3121 aggtgaattc caccatgccg cgcgctcccc gctgccgagc cgtgcgctcc ctgctgcgca
3181 gccactaccg cgaggtgctg ccgctggcca cgttcgtgcg gcgcctgggg ccccagggct
3241 ggcggctggt gcagcgcggg gacccggcgg ctttccgcgc gctggtggcc cagtgcctgg
3301 tgtgcgtgcc ctgggacgca cggccgcccc ccgccgcccc ctccttccgc caggtgtcct
3361 gcctgaagga gctggtggcc cgagtgctgc agaggctgtg cgagcgcggc gcgaagaacg
3421 tgctggcctt cggcttcgcg ctgctggacg gggcccgcgg gggccccccc gaggccttca
3481 ccaccagcgt gcgcagctac ctgcccaaca cggtgaccga cgcactgcgg gggagcgggg
3541 cgtgggggct gctgctgcgc cgcgtgggcg acgacgtgct ggttcacctg ctggcacgct
3601 gcgcgctctt tgtgctggtg gctcccagct gcgcctacca ggtgtgcggg ccgccgctgt
3661 accagctcgg cgctgccact caggcccggc ccccgccaca cgctagtgga ccccgaaggc
3721 gtctgggatg cgaacgggcc tggaaccata gcgtcaggga ggccggggtc cccctgggcc
3781 tgccagcccc gggtgcgagg aggcgcgggg gcagtgccag ccgaagtctg ccgttgccca
3841 agaggcccag gcgtggcgct gcccctgagc cggagcggac gcccgttggg caggggtcct
3901 gggcccaccc gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg gtgtcacctg
3961 ccagacccgc cgaagaagcc acctctttgg agggtgcgct ctctggcacg cgccactccc
4021 acccatccgt gggccgccag caccacgcgg gccccccatc cacatcgcgg ccaccacgtc
4081 cctgggacac gccttgtccc ccggtgtacg ccgagaccaa gcacttcctc tactcctcag
4141 gcgacaagga gcagctgcgg ccctccttcc tactcagctc tctgaggccc agcctgactg
4201 gcgctcggag gctcgtggag accatctttc tgggttccag gccctggatg ccagggactc
4261 cccgcaggtt gccccgcctg ccccagcgct actggcaaat gcggcccctg tttctggagc
4321 tgcttgggaa ccacgcgcag tgcccctacg gggtgctcct caagacgcac tgcccgctgc
4381 gagctgcggt caccccagca gccggtgtct gtgcccggga gaagccccag ggctctgtgg
4441 cggcccccga ggaggaggac acagaccccc gtcgcctggt gcagctgctc cgccagcaca
4501 gcagcccctg gcaggtgtac ggcttcgtgc gggcctgcct gcgccggctg gtgcccccag
4561 gcctctgggg ctccaggcac aacgaacgcc gcttcctcag gaacaccaag aagttcatct
4621 ccctggggaa gcatgccaag ctctcgctgc aggagctgac gtggaagatg agcgtgcggg
4681 actgcgcttg gctgcgcagg agcccagggg ttggctgtgt tccggccgca gagcaccgtc
4741 tgcgtgagga gatcctggcc aagttcctgc actggctgat gagtgtgtac gtcgtcgagc
4801 tgctcaggtc tttcttttat gtcacggaga ccacgtttca aaagaacagg ctctttttct
4861 accggaagag tgtctggagc aagttgcaaa gcattggaat cagacagcac ttgaagaggg
4921 tgcagctgcg ggagctgtcg gaagcagagg tcaggcagca tcgggaagcc aggcccgccc
4981 tgctgacgtc cagactccgc ttcatcccca agcctgacgg gctgcggccg attgtgaaca
5041 tggactacgt cgtgggagcc agaacgttcc gcagagaaaa gagggccgag cgtctcacct
5101 cgagggtgaa ggcactgttc agcgtgctca actacgagcg ggcgcggcgc cccggcctcc
5161 tgggcgcctc tgtgctgggc ctggacgata tccacagggc ctggcgcacc ttcgtgctgc
5221 gtgtgcgggc ccaggacccg ccgcctgagc tgtactttgt caaggtggat gtgacgggcg
5281 cgtacgacac catcccccag gacaggctca cggaggtcat cgccagcatc atcaaacccc
5341 agaacacgta ctgcgtgcgt cggtatgccg tggtccagaa ggccgcccat gggcacgtcc
5401 gcaaggcctt caagagccac gtctctacct tgacagacct ccagccgtac atgcgacagt
5461 tcgtggctca cctgcaggag accagcccgc tgagggatgc cgtcgtcatc gagcagagct
5521 cctccctgaa tgaggccagc agtggcctct tcgacgtctt cctacgcttc atgtgccacc
5581 acgccgtgcg catcaggggc aagtcctacg tccagtgcca ggggatcccg cagggctcca
5641 tcctctccac gctgctctgc agcctgtgct acggcgacat ggagaacaag ctgtttgcgg
5701 ggattcggcg ggacgggctg ctcctgcgtt tggtggatga tttcttgttg gtgacacctc
5761 acctcaccca cgcgaaaacc ttcctcagga ccctggtccg aggtgtccct gagtatggct
5821 gcgtggtgaa cttgcggaag acagtggtga acttccctgt agaagacgag gccctgggtg
5881 gcacggcttt tgttcagatg ccggcccacg gcctattccc ctggtgcggc ctgctgctgg
5941 atacccggac cctggaggtg cagagcgact actccagcta tgcccggacc tccatcagag
6001 ccagtctcac cttcaaccgc ggcttcaagg ctgggaggaa catgcgtcgc aaactctttg
6061 gggtcttgcg gctgaagtgt cacagcctgt ttctggattt gcaggtgaac agcctccaga
6121 cggtgtgcac caacatctac aagatcctcc tgctgcaggc gtacaggttt cacgcatgtg
6181 tgctgcagct cccatttcat cagcaagttt ggaagaaccc cacatttttc ctgcgcgtca
6241 tctctgacac ggcctccctc tgctactcca tcctgaaagc caagaacgca gggatgtcgc
6301 tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg ctgtgccacc
6361 aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc ctggggtcac
6421 tcaggacagc ccagacgcag ctgagtcgga agctcccggg gacgacgctg actgccctgg
6481 aggccgcagc caacccggca ctgccctcag acttcaagac catcctggac tgatgggacg
6541 cggccgcatg actagttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac
6601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
6661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
6721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
6781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
6841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
6901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
6961 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
7021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
7081 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc
7141 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt
7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
7561 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag
7621 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc
7681 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga
9001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
9061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
9121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
9181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
9241 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat
9301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
9361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
9421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
9481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
9541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
9601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
9661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
9721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
9781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
9841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
9901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
9961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
10021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
10081 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
10141 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg
10201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
10261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca
10321 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga
10381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
10441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
10501 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct
10561 taattaacat catcaataat atacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HincII
|
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540
CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC
541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600
GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC
601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660
ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA
HIV-1_5_LTR other(715,895)>>>
|
661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720
CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA
721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780
GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA
781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840
TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG
841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900
AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG
901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960
CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG
HIV-1_psi_pack other(1006,1050)>>>
|
961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020
CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT
1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080
AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC
NruI
|
1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140
CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT
1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200
TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG
1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260
ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT
1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320
GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG
1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380
TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT
1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440
TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC
1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500
CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT
RRE reg(1557,1790)>>>
|
1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560
CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT
ORF_1 rf(1)(1600,2301)>>>
|
1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620
CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC
1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680
GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC
1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740
TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG
1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800
GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC
1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860
CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA
1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920
TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG
1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980
TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA
1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040
CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT
2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100
TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT
2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160
TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC
2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220
AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT
2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280
CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT
2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340
TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC
2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400
TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT
2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460
TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA
2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520
CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA
2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580
GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT
NdeI
|
2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640
TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG
2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700
TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA
2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760
TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA
2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820
TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC
2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880
TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT
CMV prom(2885,2966)>>>
|
2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940
TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT
2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000
CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG
tetO reg(3022,3061)>>>
|
3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060
TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC
ClaI
|
ORF_6 rf(5)(3063,3749)<<< KpnI
| | |
3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120
TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG
EcoRI ORF_3 rf(3)(3135,6533)>>>
| |
3121 aggtgaattccaccatgccgcgcgctccccgctgccgagccgtgcgctccctgctgcgca 3180
TCCACTTAAGGTGGTACGGCGCGCGAGGGGCGACGGCTCGGCACGCGAGGGACGACGCGT
3181 gccactaccgcgaggtgctgccgctggccacgttcgtgcggcgcctggggccccagggct 3240
CGGTGATGGCGCTCCACGACGGCGACCGGTGCAAGCACGCCGCGGACCCCGGGGTCCCGA
3241 ggcggctggtgcagcgcggggacccggcggctttccgcgcgctggtggcccagtgcctgg 3300
CCGCCGACCACGTCGCGCCCCTGGGCCGCCGAAAGGCGCGCGACCACCGGGTCACGGACC
3301 tgtgcgtgccctgggacgcacggccgccccccgccgccccctccttccgccaggtgtcct 3360
ACACGCACGGGACCCTGCGTGCCGGCGGGGGGCGGCGGGGGAGGAAGGCGGTCCACAGGA
3361 gcctgaaggagctggtggcccgagtgctgcagaggctgtgcgagcgcggcgcgaagaacg 3420
CGGACTTCCTCGACCACCGGGCTCACGACGTCTCCGACACGCTCGCGCCGCGCTTCTTGC
3421 tgctggccttcggcttcgcgctgctggacggggcccgcgggggcccccccgaggccttca 3480
ACGACCGGAAGCCGAAGCGCGACGACCTGCCCCGGGCGCCCCCGGGGGGGCTCCGGAAGT
3481 ccaccagcgtgcgcagctacctgcccaacacggtgaccgacgcactgcgggggagcgggg 3540
GGTGGTCGCACGCGTCGATGGACGGGTTGTGCCACTGGCTGCGTGACGCCCCCTCGCCCC
3541 cgtgggggctgctgctgcgccgcgtgggcgacgacgtgctggttcacctgctggcacgct 3600
GCACCCCCGACGACGACGCGGCGCACCCGCTGCTGCACGACCAAGTGGACGACCGTGCGA
3601 gcgcgctctttgtgctggtggctcccagctgcgcctaccaggtgtgcgggccgccgctgt 3660
CGCGCGAGAAACACGACCACCGAGGGTCGACGCGGATGGTCCACACGCCCGGCGGCGACA
3661 accagctcggcgctgccactcaggcccggcccccgccacacgctagtggaccccgaaggc 3720
TGGTCGAGCCGCGACGGTGAGTCCGGGCCGGGGGCGGTGTGCGATCACCTGGGGCTTCCG
3721 gtctgggatgcgaacgggcctggaaccatagcgtcagggaggccggggtccccctgggcc 3780
CAGACCCTACGCTTGCCCGGACCTTGGTATCGCAGTCCCTCCGGCCCCAGGGGGACCCGG
3781 tgccagccccgggtgcgaggaggcgcgggggcagtgccagccgaagtctgccgttgccca 3840
ACGGTCGGGGCCCACGCTCCTCCGCGCCCCCGTCACGGTCGGCTTCAGACGGCAACGGGT
3841 agaggcccaggcgtggcgctgcccctgagccggagcggacgcccgttgggcaggggtcct 3900
TCTCCGGGTCCGCACCGCGACGGGGACTCGGCCTCGCCTGCGGGCAACCCGTCCCCAGGA
3901 gggcccacccgggcaggacgcgtggaccgagtgaccgtggtttctgtgtggtgtcacctg 3960
CCCGGGTGGGCCCGTCCTGCGCACCTGGCTCACTGGCACCAAAGACACACCACAGTGGAC
3961 ccagacccgccgaagaagccacctctttggagggtgcgctctctggcacgcgccactccc 4020
GGTCTGGGCGGCTTCTTCGGTGGAGAAACCTCCCACGCGAGAGACCGTGCGCGGTGAGGG
4021 acccatccgtgggccgccagcaccacgcgggccccccatccacatcgcggccaccacgtc 4080
TGGGTAGGCACCCGGCGGTCGTGGTGCGCCCGGGGGGTAGGTGTAGCGCCGGTGGTGCAG
4081 cctgggacacgccttgtcccccggtgtacgccgagaccaagcacttcctctactcctcag 4140
GGACCCTGTGCGGAACAGGGGGCCACATGCGGCTCTGGTTCGTGAAGGAGATGAGGAGTC
ORF_5 rf(5)(4170,5390)<<<
|
4141 gcgacaaggagcagctgcggccctccttcctactcagctctctgaggcccagcctgactg 4200
CGCTGTTCCTCGTCGACGCCGGGAGGAAGGATGAGTCGAGAGACTCCGGGTCGGACTGAC
4201 gcgctcggaggctcgtggagaccatctttctgggttccaggccctggatgccagggactc 4260
CGCGAGCCTCCGAGCACCTCTGGTAGAAAGACCCAAGGTCCGGGACCTACGGTCCCTGAG
4261 cccgcaggttgccccgcctgccccagcgctactggcaaatgcggcccctgtttctggagc 4320
GGGCGTCCAACGGGGCGGACGGGGTCGCGATGACCGTTTACGCCGGGGACAAAGACCTCG
4321 tgcttgggaaccacgcgcagtgcccctacggggtgctcctcaagacgcactgcccgctgc 4380
ACGAACCCTTGGTGCGCGTCACGGGGATGCCCCACGAGGAGTTCTGCGTGACGGGCGACG
4381 gagctgcggtcaccccagcagccggtgtctgtgcccgggagaagccccagggctctgtgg 4440
CTCGACGCCAGTGGGGTCGTCGGCCACAGACACGGGCCCTCTTCGGGGTCCCGAGACACC
4441 cggcccccgaggaggaggacacagacccccgtcgcctggtgcagctgctccgccagcaca 4500
GCCGGGGGCTCCTCCTCCTGTGTCTGGGGGCAGCGGACCACGTCGACGAGGCGGTCGTGT
4501 gcagcccctggcaggtgtacggcttcgtgcgggcctgcctgcgccggctggtgcccccag 4560
CGTCGGGGACCGTCCACATGCCGAAGCACGCCCGGACGGACGCGGCCGACCACGGGGGTC
4561 gcctctggggctccaggcacaacgaacgccgcttcctcaggaacaccaagaagttcatct 4620
CGGAGACCCCGAGGTCCGTGTTGCTTGCGGCGAAGGAGTCCTTGTGGTTCTTCAAGTAGA
4621 ccctggggaagcatgccaagctctcgctgcaggagctgacgtggaagatgagcgtgcggg 4680
GGGACCCCTTCGTACGGTTCGAGAGCGACGTCCTCGACTGCACCTTCTACTCGCACGCCC
4681 actgcgcttggctgcgcaggagcccaggggttggctgtgttccggccgcagagcaccgtc 4740
TGACGCGAACCGACGCGTCCTCGGGTCCCCAACCGACACAAGGCCGGCGTCTCGTGGCAG
4741 tgcgtgaggagatcctggccaagttcctgcactggctgatgagtgtgtacgtcgtcgagc 4800
ACGCACTCCTCTAGGACCGGTTCAAGGACGTGACCGACTACTCACACATGCAGCAGCTCG
4801 tgctcaggtctttcttttatgtcacggagaccacgtttcaaaagaacaggctctttttct 4860
ACGAGTCCAGAAAGAAAATACAGTGCCTCTGGTGCAAAGTTTTCTTGTCCGAGAAAAAGA
4861 accggaagagtgtctggagcaagttgcaaagcattggaatcagacagcacttgaagaggg 4920
TGGCCTTCTCACAGACCTCGTTCAACGTTTCGTAACCTTAGTCTGTCGTGAACTTCTCCC
4921 tgcagctgcgggagctgtcggaagcagaggtcaggcagcatcgggaagccaggcccgccc 4980
ACGTCGACGCCCTCGACAGCCTTCGTCTCCAGTCCGTCGTAGCCCTTCGGTCCGGGCGGG
4981 tgctgacgtccagactccgcttcatccccaagcctgacgggctgcggccgattgtgaaca 5040
ACGACTGCAGGTCTGAGGCGAAGTAGGGGTTCGGACTGCCCGACGCCGGCTAACACTTGT
5041 tggactacgtcgtgggagccagaacgttccgcagagaaaagagggccgagcgtctcacct 5100
ACCTGATGCAGCACCCTCGGTCTTGCAAGGCGTCTCTTTTCTCCCGGCTCGCAGAGTGGA
XhoI
|
5101 cgagggtgaaggcactgttcagcgtgctcaactacgagcgggcgcggcgccccggcctcc 5160
GCTCCCACTTCCGTGACAAGTCGCACGAGTTGATGCTCGCCCGCGCCGCGGGGCCGGAGG
EcoRV
|
5161 tgggcgcctctgtgctgggcctggacgatatccacagggcctggcgcaccttcgtgctgc 5220
ACCCGCGGAGACACGACCCGGACCTGCTATAGGTGTCCCGGACCGCGTGGAAGCACGACG
5221 gtgtgcgggcccaggacccgccgcctgagctgtactttgtcaaggtggatgtgacgggcg 5280
CACACGCCCGGGTCCTGGGCGGCGGACTCGACATGAAACAGTTCCACCTACACTGCCCGC
5281 cgtacgacaccatcccccaggacaggctcacggaggtcatcgccagcatcatcaaacccc 5340
GCATGCTGTGGTAGGGGGTCCTGTCCGAGTGCCTCCAGTAGCGGTCGTAGTAGTTTGGGG
5341 agaacacgtactgcgtgcgtcggtatgccgtggtccagaaggccgcccatgggcacgtcc 5400
TCTTGTGCATGACGCACGCAGCCATACGGCACCAGGTCTTCCGGCGGGTACCCGTGCAGG
5401 gcaaggccttcaagagccacgtctctaccttgacagacctccagccgtacatgcgacagt 5460
CGTTCCGGAAGTTCTCGGTGCAGAGATGGAACTGTCTGGAGGTCGGCATGTACGCTGTCA
5461 tcgtggctcacctgcaggagaccagcccgctgagggatgccgtcgtcatcgagcagagct 5520
AGCACCGAGTGGACGTCCTCTGGTCGGGCGACTCCCTACGGCAGCAGTAGCTCGTCTCGA
5521 cctccctgaatgaggccagcagtggcctcttcgacgtcttcctacgcttcatgtgccacc 5580
GGAGGGACTTACTCCGGTCGTCACCGGAGAAGCTGCAGAAGGATGCGAAGTACACGGTGG
BamHI
|
5581 acgccgtgcgcatcaggggcaagtcctacgtccagtgccaggggatcccgcagggctcca 5640
TGCGGCACGCGTAGTCCCCGTTCAGGATGCAGGTCACGGTCCCCTAGGGCGTCCCGAGGT
5641 tcctctccacgctgctctgcagcctgtgctacggcgacatggagaacaagctgtttgcgg 5700
AGGAGAGGTGCGACGAGACGTCGGACACGATGCCGCTGTACCTCTTGTTCGACAAACGCC
5701 ggattcggcgggacgggctgctcctgcgtttggtggatgatttcttgttggtgacacctc 5760
CCTAAGCCGCCCTGCCCGACGAGGACGCAAACCACCTACTAAAGAACAACCACTGTGGAG
5761 acctcacccacgcgaaaaccttcctcaggaccctggtccgaggtgtccctgagtatggct 5820
TGGAGTGGGTGCGCTTTTGGAAGGAGTCCTGGGACCAGGCTCCACAGGGACTCATACCGA
5821 gcgtggtgaacttgcggaagacagtggtgaacttccctgtagaagacgaggccctgggtg 5880
CGCACCACTTGAACGCCTTCTGTCACCACTTGAAGGGACATCTTCTGCTCCGGGACCCAC
5881 gcacggcttttgttcagatgccggcccacggcctattcccctggtgcggcctgctgctgg 5940
CGTGCCGAAAACAAGTCTACGGCCGGGTGCCGGATAAGGGGACCACGCCGGACGACGACC
5941 atacccggaccctggaggtgcagagcgactactccagctatgcccggacctccatcagag 6000
TATGGGCCTGGGACCTCCACGTCTCGCTGATGAGGTCGATACGGGCCTGGAGGTAGTCTC
6001 ccagtctcaccttcaaccgcggcttcaaggctgggaggaacatgcgtcgcaaactctttg 6060
GGTCAGAGTGGAAGTTGGCGCCGAAGTTCCGACCCTCCTTGTACGCAGCGTTTGAGAAAC
6061 gggtcttgcggctgaagtgtcacagcctgtttctggatttgcaggtgaacagcctccaga 6120
CCCAGAACGCCGACTTCACAGTGTCGGACAAAGACCTAAACGTCCACTTGTCGGAGGTCT
6121 cggtgtgcaccaacatctacaagatcctcctgctgcaggcgtacaggtttcacgcatgtg 6180
GCCACACGTGGTTGTAGATGTTCTAGGAGGACGACGTCCGCATGTCCAAAGTGCGTACAC
6181 tgctgcagctcccatttcatcagcaagtttggaagaaccccacatttttcctgcgcgtca 6240
ACGACGTCGAGGGTAAAGTAGTCGTTCAAACCTTCTTGGGGTGTAAAAAGGACGCGCAGT
6241 tctctgacacggcctccctctgctactccatcctgaaagccaagaacgcagggatgtcgc 6300
AGAGACTGTGCCGGAGGGAGACGATGAGGTAGGACTTTCGGTTCTTGCGTCCCTACAGCG
6301 tgggggccaagggcgccgccggccctctgccctccgaggccgtgcagtggctgtgccacc 6360
ACCCCCGGTTCCCGCGGCGGCCGGGAGACGGGAGGCTCCGGCACGTCACCGACACGGTGG
6361 aagcattcctgctcaagctgactcgacaccgtgtcacctacgtgccactcctggggtcac 6420
TTCGTAAGGACGAGTTCGACTGAGCTGTGGCACAGTGGATGCACGGTGAGGACCCCAGTG
6421 tcaggacagcccagacgcagctgagtcggaagctcccggggacgacgctgactgccctgg 6480
AGTCCTGTCGGGTCTGCGTCGACTCAGCCTTCGAGGGCCCCTGCTGCGACTGACGGGACC
ORF_2 rf(2)(6533,7171)>>>
|
6481 aggccgcagccaacccggcactgccctcagacttcaagaccatcctggactgatgggacg 6540
TCCGGCGTCGGTTGGGCCGTGACGGGAGTCTGAAGTTCTGGTAGGACCTGACTACCCTGC
NotI
|
6541 cggccgcatgactagttccggaaatcaacctctggattacaaaatttgtgaaagattgac 6600
GCCGGCGTACTGATCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTG
6601 tggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgccttt 6660
ACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAA
6661 gtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggtt 6720
CATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAA
6721 gctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgt 6780
CGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACA
6781 gtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgg 6840
CAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCC
6841 gactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccg 6900
CTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGC
6901 ctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagct 6960
GACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGA
6961 gacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtcctt 7020
CTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAA
7021 ctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggc 7080
GACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCG
7081 tctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggcc 7140
AGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGG
delta_U3 other(7160,7212)>>>
|
7141 gcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgctt 7200
CGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAA
HIV-1_5_LTR other(7213,7393)>>>
|
7201 tttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaa 7260
AAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATT
7261 ctagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgt 7320
GATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACA
7321 gcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtgg 7380
CGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACC
7381 aaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaag 7440
TTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTC
7441 aaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataa 7500
TTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATT
7501 agcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggt 7560
TCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCA
7561 ttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaag 7620
AACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTC
7621 aggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcc 7680
TCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGG
7681 cggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgct 7740
GCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGA
7741 tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac 7800
AGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTG
7801 tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtga 7860
AGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACT
pBR322 origin(7899,8518)<<<
|
7861 gcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccat 7920
CGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTA
7921 aggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaac 7980
TCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTG
7981 ccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct 8040
GGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGA
8041 gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg 8100
CAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGC
8101 ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctg 8160
GAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGAC
8161 ggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgt 8220
CCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCA
8221 cttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacagg 8280
GAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCC
8281 attagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac 8340
TAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATG
8341 ggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga 8400
CCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCT
8401 aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt 8460
TTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAA
8461 gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttt 8520
CAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAA
8521 tctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgaga 8580
AGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCT
8581 ttatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc 8640
AATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAG
8641 taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct 8700
ATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGA
8701 atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagata 8760
TAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTAT
8761 actacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaccca 8820
TGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGT
8821 cgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga 8880
GCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCT
8881 agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga 8940
TCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCT
8941 gtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctaga 9000
CATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCT
9001 tccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagct 9060
AGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGA
9061 actgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtg 9120
TGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCAC
NEOKAN prom(9171,9220)>>>
|
9121 ggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgc 9180
CCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACG
9181 cagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttct 9240
GTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGA
BclI
|
9241 cgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggat 9300
GCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTA
NTP_II marker(9312,10100)>>>
|
ORF_4 rf(3)(9309,10103)>>>
| |
9301 cgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggaga 9360
GCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCT
9361 ggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttcc 9420
CCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGG
9421 ggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctga 9480
CCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACT
9481 atgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg 9540
TACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGC
9541 cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgc 9600
GTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACG
9601 cggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctg 9660
GCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGAC
9661 atgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcga 9720
TACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCT
9721 aacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatc 9780
TTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAG
9781 tggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagca 9840
ACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGT
9841 tgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatgg 9900
ACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACC
9901 tggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgct 9960
ACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGA
9961 atcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctg 10020
TAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGAC
10021 accgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatc 10080
TGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAG
10081 gccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcatttttt 10140
CGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAA
10141 aaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgataggg 10200
TTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCC
f1 origin(10208,10513)<<<
|
10201 ttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtc 10260
AACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAG
10261 aaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatca 10320
TTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGT
10321 agttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccga 10380
TCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCT
10381 tttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaa 10440
AAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTT
10441 ggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacaccc 10500
CCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGG
10501 gcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattct 10560
CGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGA
10561 taattaacatcatcaataatatacctt 10587
ATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10587 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
Other Gene 715..895
/gene="HIV-1_5_LTR other"
Other Gene 1006..1050
/gene="HIV-1_psi_pack other"
misc_binding 1092..1097
/dbxref="REBASE:NruI"
Regulatory_Seq 1557..1790
/gene="RRE reg"
ORF 1600..2301
/sequence="ORF_1 rf(1)"
misc_binding 2611..2616
/dbxref="REBASE:NdeI"
Promoter 2885..2966
/gene="CMV prom"
Regulatory_Seq 3022..3061
/gene="tetO reg"
ORF 3063..3749
/sequence="ORF_6 rf(5)"
misc_binding 3074..3079
/dbxref="REBASE:ClaI"
misc_binding 3098..3103
/dbxref="REBASE:KpnI"
misc_binding 3125..3130
/dbxref="REBASE:EcoRI"
ORF 3135..6533
/sequence="ORF_3 rf(3)"
ORF 4170..5390
/sequence="ORF_5 rf(5)"
misc_binding 5099..5104
/dbxref="REBASE:XhoI"
misc_binding 5187..5192
/dbxref="REBASE:EcoRV"
misc_binding 5623..5628
/dbxref="REBASE:BamHI"
ORF 6533..7171
/sequence="ORF_2 rf(2)"
misc_binding 6540..6547
/dbxref="REBASE:NotI"
Other Gene 7160..7212
/gene="delta_U3 other"
Other Gene 7213..7393
/gene="HIV-1_5_LTR other"
Rep_Origin 7899..8518
/gene="pBR322 origin"
Promoter 9171..9220
/gene="NEOKAN prom"
misc_binding 9278..9283
/dbxref="REBASE:BclI"
ORF 9309..10103
/sequence="ORF_4 rf(3)"
Marker 9312..10100
/gene="NTP_II marker"
Rep_Origin 10208..10513
/gene="f1 origin"