pLenti-promoter-GFP

Vector Name pLenti-promoter-GFP
VectorType Lentivirus
Antibiotic Information Puromycin
Sequencing Primers
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga     
     1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca     
     1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt     
     1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc     
     2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac     
     2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc gctagggggc     
     2161 agcagcgacc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc cgagccggca     
     2221 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct     
     2281 tctcgctgct ctttgagcct gcagacacct gggggatacg gggaaaagaa ttgggggagc     
     2341 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag     
     2401 ggggcagcag cgaccgcccg gggctccgct ccggtccggc gctccccccc atcccgagcc     
     2461 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct tcctctgaac     
     2521 gcttctcgct gctctttgag cctgcagaca cctgggggat acggggaaaa acactggggt     
     2581 acctacgtaa gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac     
     2641 gtccctcccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc     
     2701 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg     
     2761 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg     
     2821 gggaaaagaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg cggccgctgg     
     2881 atcctctaga ctgcagctcg agatggtgag caagggcgag gagctgttca ccggggtggt     
     2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga     
     3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     
     3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     
     3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     
     3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     
     3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     
     3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     
     3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     
     3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     
     3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     
     3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     
     3601 catggacgag ctgtacaagg ggcaccacca ccaccaccac tgagtcgagt acccatacga     
     3661 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag     
     3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc     
     3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag     
     3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc     
     3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc     
     3961 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg     
     4021 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc     
     4081 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag     
     4141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac     
     4201 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc     
     4261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag     
     4321 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc     
     4381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg     
     4441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc     
     4501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg     
     4561 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg     
     4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta     
     4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt     
     4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc     
     4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt     
     4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg     
     4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac     
     4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc     
     5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac     
     5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc     
     5161 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc     
     5221 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga     
     5281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct     
     5341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat     
     5401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta     
     5461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta     
     5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat     
     5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct     
     5641 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc     
     5701 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga     
     5761 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     
     5821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     
     5881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     
     5941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     
     6001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     
     6061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     
     6121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     
     6181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     
     6241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     
     6301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     
     6361 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     
     6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     
     6481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     
     6541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     
     6601 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc     
     6661 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct     
     6721 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca     
     6781 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg     
     6841 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca     
     6901 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc     
     6961 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     
     7021 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     
     7081 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     
     7141 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     
     7201 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     
     7261 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     
     7321 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     
     7381 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     
     7441 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     
     7501 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     
     7561 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     
     7621 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     
     7681 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     
     7741 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata     
     7801 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt     
     7861 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg     
     7921 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt     
     7981 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc     
     8041 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg     
     8101 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct     
     8161 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa     
     8221 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt     
     8281 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg     
     8341 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg     
     8401 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt     
     8461 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa     
     8521 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat     
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     
     8641 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc     
     8701 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag     
     8761 caccgtgcat gccgattggt ggaagtaagg tggtacgat                            
                            LOCUS       dna                      8799 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     misc_binding    486..491
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     misc_binding    1833..1838
                     /dbxref="REBASE:ClaI"
     ORF             2341..2946
                     /sequence="ORF_5 rf(4)"
     ORF             2597..3742
                     /sequence="ORF_6 rf(6)"
     misc_binding    2828..2833
                     /dbxref="REBASE:EcoRI"
     misc_binding    2846..2851
                     /dbxref="REBASE:StuI"
     misc_binding    2852..2857
                     /dbxref="REBASE:HincII"
     misc_binding    2858..2863
                     /dbxref="REBASE:EcoRV"
     misc_binding    2864..2869
                     /dbxref="REBASE:ApaI"
     misc_binding    2870..2877
                     /dbxref="REBASE:NotI"
     misc_binding    2879..2884
                     /dbxref="REBASE:BamHI"
     misc_binding    2885..2890
                     /dbxref="REBASE:XbaI"
     misc_binding    2897..2902
                     /dbxref="REBASE:XhoI"
     Reporter        2903..3619
                     /gene="EGFP reporter"
     ORF             2903..3643
                     /sequence="ORF_4 rf(2)"
     Promoter        3694..3962
                     /gene="SV40 prom"
     Rep_Origin      3861..3938
                     /gene="SV40 origin"
     ORF             3928..4596
                     /sequence="ORF_2 rf(1)"
     misc_binding    3941..3953
                     /dbxref="REBASE:SfiI"
     Marker          3997..4596
                     /gene="puro marker"
     Other Gene      5206..5258
                     /gene="delta_U3 other"
     Other Gene      5259..5439
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5946..6565
                     /gene="pBR322 origin"
     Promoter        6823..6872
                     /gene="NEOKAN prom"
     misc_binding    6930..6935
                     /dbxref="REBASE:BclI"
     ORF             6961..7755
                     /sequence="ORF_3 rf(1)"
     Marker          6964..7752
                     /gene="NTP_II marker"
     Rep_Origin      7860..8166
                     /gene="f1 origin"
     Other Gene      8221..8322
                     /gene="RITR other"
     Other Gene      8221..8322
                     /gene="LITR other"
     Other Gene      8402..8550
                     /gene="Encap other"
BASE COUNT    2113 a   2345 c   2495 g   1846 t    0 others
ORIGIN
        1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg     
       61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg     
      121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct     
      181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt     
      241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga     
      301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg     
      361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga     
      421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa     
      481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa     
      541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag     
      601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat     
      661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     
      721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     
      781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat     
      841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc     
      901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg     
      961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg     
     1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct     
     1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca     
     1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc     
     1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct     
     1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac     
     1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa     
     1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg     
     1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt     
     1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag     
     1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga     
     1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt     
     1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt     
     1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca     
     1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga     
     1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca     
     1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt     
     1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc     
     2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac     
     2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc gctagggggc     
     2161 agcagcgacc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc cgagccggca     
     2221 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct     
     2281 tctcgctgct ctttgagcct gcagacacct gggggatacg gggaaaagaa ttgggggagc     
     2341 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag     
     2401 ggggcagcag cgaccgcccg gggctccgct ccggtccggc gctccccccc atcccgagcc     
     2461 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct tcctctgaac     
     2521 gcttctcgct gctctttgag cctgcagaca cctgggggat acggggaaaa acactggggt     
     2581 acctacgtaa gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac     
     2641 gtccctcccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc     
     2701 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg     
     2761 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg     
     2821 gggaaaagaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg cggccgctgg     
     2881 atcctctaga ctgcagctcg agatggtgag caagggcgag gagctgttca ccggggtggt     
     2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga     
     3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa     
     3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag     
     3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta     
     3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt     
     3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga     
     3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat     
     3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga     
     3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc     
     3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa     
     3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg     
     3601 catggacgag ctgtacaagg ggcaccacca ccaccaccac tgagtcgagt acccatacga     
     3661 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag     
     3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc     
     3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag     
     3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc     
     3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc     
     3961 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg     
     4021 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc     
     4081 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag     
     4141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac     
     4201 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc     
     4261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag     
     4321 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc     
     4381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg     
     4441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc     
     4501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg     
     4561 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg     
     4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta     
     4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt     
     4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc     
     4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt     
     4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg     
     4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac     
     4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc     
     5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac     
     5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc     
     5161 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc     
     5221 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga     
     5281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct     
     5341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat     
     5401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta     
     5461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta     
     5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat     
     5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct     
     5641 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc     
     5701 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga     
     5761 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     
     5821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     
     5881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     
     5941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     
     6001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     
     6061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     
     6121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     
     6181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     
     6241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     
     6301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     
     6361 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc     
     6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     
     6481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     
     6541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     
     6601 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc     
     6661 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct     
     6721 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca     
     6781 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg     
     6841 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca     
     6901 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc     
     6961 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc     
     7021 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca     
     7081 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg     
     7141 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg     
     7201 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag     
     7261 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg     
     7321 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc     
     7381 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa     
     7441 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac     
     7501 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat     
     7561 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac     
     7621 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc     
     7681 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt     
     7741 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata     
     7801 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt     
     7861 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg     
     7921 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt     
     7981 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc     
     8041 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg     
     8101 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct     
     8161 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa     
     8221 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt     
     8281 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg     
     8341 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg     
     8401 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt     
     8461 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa     
     8521 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat     
     8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     
     8641 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc     
     8701 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag     
     8761 caccgtgcat gccgattggt ggaagtaagg tggtacgat                            
//

                        
                            LOCUS       dna                      8799 bp                                   
FEATURES             Location/Qualifiers
     Other Gene      109..289
                     /gene="HIV-1_5_LTR other"
     Other Gene      400..444
                     /gene="HIV-1_psi_pack other"
     misc_binding    486..491
                     /dbxref="REBASE:NruI"
     Regulatory_Seq  951..1184
                     /gene="RRE reg"
     ORF             994..1695
                     /sequence="ORF_1 rf(1)"
     misc_binding    1833..1838
                     /dbxref="REBASE:ClaI"
     ORF             2341..2946
                     /sequence="ORF_5 rf(4)"
     ORF             2597..3742
                     /sequence="ORF_6 rf(6)"
     misc_binding    2828..2833
                     /dbxref="REBASE:EcoRI"
     misc_binding    2846..2851
                     /dbxref="REBASE:StuI"
     misc_binding    2852..2857
                     /dbxref="REBASE:HincII"
     misc_binding    2858..2863
                     /dbxref="REBASE:EcoRV"
     misc_binding    2864..2869
                     /dbxref="REBASE:ApaI"
     misc_binding    2870..2877
                     /dbxref="REBASE:NotI"
     misc_binding    2879..2884
                     /dbxref="REBASE:BamHI"
     misc_binding    2885..2890
                     /dbxref="REBASE:XbaI"
     misc_binding    2897..2902
                     /dbxref="REBASE:XhoI"
     Reporter        2903..3619
                     /gene="EGFP reporter"
     ORF             2903..3643
                     /sequence="ORF_4 rf(2)"
     Promoter        3694..3962
                     /gene="SV40 prom"
     Rep_Origin      3861..3938
                     /gene="SV40 origin"
     ORF             3928..4596
                     /sequence="ORF_2 rf(1)"
     misc_binding    3941..3953
                     /dbxref="REBASE:SfiI"
     Marker          3997..4596
                     /gene="puro marker"
     Other Gene      5206..5258
                     /gene="delta_U3 other"
     Other Gene      5259..5439
                     /gene="HIV-1_5_LTR other"
     Rep_Origin      5946..6565
                     /gene="pBR322 origin"
     Promoter        6823..6872
                     /gene="NEOKAN prom"
     misc_binding    6930..6935
                     /dbxref="REBASE:BclI"
     ORF             6961..7755
                     /sequence="ORF_3 rf(1)"
     Marker          6964..7752
                     /gene="NTP_II marker"
     Rep_Origin      7860..8166
                     /gene="f1 origin"
     Other Gene      8221..8322
                     /gene="RITR other"
     Other Gene      8221..8322
                     /gene="LITR other"
     Other Gene      8402..8550
                     /gene="Encap other"
BASE COUNT    2113 a   2345 c   2495 g   1846 t    0 others