pRetro-SV40LT
| Vector Name | pRetro-SV40LT |
| VectorType | Retroviral Plasmid |
| Antibiotic Information | Mammalian: Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
2881 cggactcaga tctaagcttg ccaccatgga taaagtttta aacagagagg aatctttgca
2941 gctaatggac cttctaggtc ttgaaaggag tgcctggggg aatattcctc tgatgagaaa
3001 ggcatattta aaaaaatgca aggagtttca tcctgataaa ggaggagatg aagaaaaaat
3061 gaagaaaatg aatactctgt acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc
3121 tgactttgga ggcttctggg atgcaactga gattccaacc tatggaactg atgaatggga
3181 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
3241 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
3301 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
3361 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
3421 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
3481 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
3541 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
3601 gtatagtgcc ttgactagag atccattttc tgttattgag gaaagtttgc caggtgggtt
3661 aaaggagcat gattttaatc cagaagaagc agaggaaact aaacaagtgt cctggaagct
3721 tgtaacagag tatgcaatgg aaacaaaatg tgatgatgtg ttgttattgc ttgggatgta
3781 cttggaattt cagtacagtt ttgaaatgtg tttaaaatgt attaaaaaag aacagcccag
3841 ccactataag taccatgaaa agcattatgc aaatgctgct atatttgctg acagcaaaaa
3901 ccaaaaaacc atatgccaac aggctgttga tactgtttta gctaaaaagc gggttgatag
3961 cctacaatta actagagaac aaatgttaac aaacagattt aatgatcttt tggataggat
4021 ggatataatg tttggttcta caggctctgc tgacatagaa gaatggatgg ctggagttgc
4081 ttggctacac tgtttgttgc ccaaaatgga ttcagtggtg tatgactttt taaaatgcat
4141 ggtgtacaac attcctaaaa aaagatactg gctgtttaaa ggaccaattg atagtggtaa
4201 aactacatta gcagctgctt tgcttgaatt atgtgggggg aaagctttaa atgttaattt
4261 gcccttggac aggctgaact ttgagctagg agtagctatt gaccagtttt tagtagtttt
4321 tgaggatgta aagggcactg gaggggagtc cagagatttg ccttcaggtc agggaattaa
4381 taacctggac aatttaaggg attatttgga tggcagtgtt aaggtaaact tagaaaagaa
4441 acacctaaat aaaagaactc aaatatttcc ccctggaata gtcaccatga atgagtacag
4501 tgtgcctaaa acactgcagg ccagatttgt aaaacaaata gattttaggc ccaaagatta
4561 tttaaagcat tgcctggaac gcagtgagtt tttgttagaa aagagaataa ttcaaagtgg
4621 cattgctttg cttcttatgt taatttggta cagacctgtg gctgagtttg ctcaaagtat
4681 tcagagcaga attgtggagt ggaaagagag attggacaaa gagtttagtt tgtcagtgta
4741 tcaaaaaatg aagtttaatg tggctatggg aattggagtt ttagattggc taagaaacag
4801 tgatgatgat gatgaagaca gccaggaaaa tgctgataaa aatgaagatg gtggggagaa
4861 gaacatggaa gactcagggc atgaaacagg cattgattca cagtcccaag gctcatttca
4921 ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca tttgtagagg
4981 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aagcggccgc
5041 ctcggccaaa catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
5101 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
5161 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
5221 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
5281 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
5341 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
5401 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
5461 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
5521 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
5581 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
5641 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
5701 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
5761 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
5821 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
5881 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtac
5941 tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc
6001 cagccaagct agggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata
6061 cgaagttatc agtactggct taactatgcg gcatcagagc agattgtact gagagtgcac
6121 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6781 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
8101 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
8161 atcacgaggc cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc
8221 ataccagatc accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg
8281 gcttctgcct cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg
8341 cccttccgtt tctttgct
//
LOCUS dna 8358 bp
FEATURES Location/Qualifiers
Other Gene 659..1468
/gene="psi_plus_pack other"
ORF 1449..2216
/sequence="ORF_3 rf(5)"
Marker 1566..2165
/gene="puro marker"
misc_binding 1674..1679
/dbxref="REBASE:AccI"
misc_binding 1895..1900
/dbxref="REBASE:StuI"
Promoter 2787..2868
/gene="CMV prom"
misc_binding 2888..2893
/dbxref="REBASE:BglII"
ORF 2906..5032
/sequence="ORF_1 rf(2)"
Other Gene 3164..5032
/gene="SV40_lrgT other"
misc_binding 3985..3990
/dbxref="REBASE:HpaI"
misc_binding 4948..4953
/dbxref="REBASE:BclI"
misc_binding 5033..5040
/dbxref="REBASE:NotI"
misc_binding 5035..5047
/dbxref="REBASE:SfiI"
misc_binding 5052..5057
/dbxref="REBASE:ClaI"
Other Gene 5139..5690
/gene="5_LTR2 other"
misc_binding 5394..5399
/dbxref="REBASE:XbaI"
Other Gene 5943..5976
/gene="loxP other"
Other Gene 6036..6069
/gene="loxP other"
Rep_Origin 6345..6964
/gene="pBR322 origin"
Marker 7119..7979
/gene="amp marker"
ORF 7119..7979
/sequence="ORF_2 rf(5)"
Promoter 8021..8049
/gene="amp prom"
BASE COUNT 2144 a 2123 c 2071 g 2020 t 0 others
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
2881 cggactcaga tctaagcttg ccaccatgga taaagtttta aacagagagg aatctttgca
2941 gctaatggac cttctaggtc ttgaaaggag tgcctggggg aatattcctc tgatgagaaa
3001 ggcatattta aaaaaatgca aggagtttca tcctgataaa ggaggagatg aagaaaaaat
3061 gaagaaaatg aatactctgt acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc
3121 tgactttgga ggcttctggg atgcaactga gattccaacc tatggaactg atgaatggga
3181 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
3241 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
3301 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
3361 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
3421 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
3481 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
3541 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
3601 gtatagtgcc ttgactagag atccattttc tgttattgag gaaagtttgc caggtgggtt
3661 aaaggagcat gattttaatc cagaagaagc agaggaaact aaacaagtgt cctggaagct
3721 tgtaacagag tatgcaatgg aaacaaaatg tgatgatgtg ttgttattgc ttgggatgta
3781 cttggaattt cagtacagtt ttgaaatgtg tttaaaatgt attaaaaaag aacagcccag
3841 ccactataag taccatgaaa agcattatgc aaatgctgct atatttgctg acagcaaaaa
3901 ccaaaaaacc atatgccaac aggctgttga tactgtttta gctaaaaagc gggttgatag
3961 cctacaatta actagagaac aaatgttaac aaacagattt aatgatcttt tggataggat
4021 ggatataatg tttggttcta caggctctgc tgacatagaa gaatggatgg ctggagttgc
4081 ttggctacac tgtttgttgc ccaaaatgga ttcagtggtg tatgactttt taaaatgcat
4141 ggtgtacaac attcctaaaa aaagatactg gctgtttaaa ggaccaattg atagtggtaa
4201 aactacatta gcagctgctt tgcttgaatt atgtgggggg aaagctttaa atgttaattt
4261 gcccttggac aggctgaact ttgagctagg agtagctatt gaccagtttt tagtagtttt
4321 tgaggatgta aagggcactg gaggggagtc cagagatttg ccttcaggtc agggaattaa
4381 taacctggac aatttaaggg attatttgga tggcagtgtt aaggtaaact tagaaaagaa
4441 acacctaaat aaaagaactc aaatatttcc ccctggaata gtcaccatga atgagtacag
4501 tgtgcctaaa acactgcagg ccagatttgt aaaacaaata gattttaggc ccaaagatta
4561 tttaaagcat tgcctggaac gcagtgagtt tttgttagaa aagagaataa ttcaaagtgg
4621 cattgctttg cttcttatgt taatttggta cagacctgtg gctgagtttg ctcaaagtat
4681 tcagagcaga attgtggagt ggaaagagag attggacaaa gagtttagtt tgtcagtgta
4741 tcaaaaaatg aagtttaatg tggctatggg aattggagtt ttagattggc taagaaacag
4801 tgatgatgat gatgaagaca gccaggaaaa tgctgataaa aatgaagatg gtggggagaa
4861 gaacatggaa gactcagggc atgaaacagg cattgattca cagtcccaag gctcatttca
4921 ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca tttgtagagg
4981 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aagcggccgc
5041 ctcggccaaa catcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
5101 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
5161 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
5221 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
5281 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
5341 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
5401 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
5461 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
5521 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
5581 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
5641 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
5701 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
5761 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
5821 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
5881 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtac
5941 tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc
6001 cagccaagct agggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata
6061 cgaagttatc agtactggct taactatgcg gcatcagagc agattgtact gagagtgcac
6121 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6781 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
8101 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
8161 atcacgaggc cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc
8221 ataccagatc accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg
8281 gcttctgcct cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg
8341 cccttccgtt tctttgct
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcatggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagctgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaacagatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccggctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaatcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaaagagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtacccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgtccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggccagcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcgtctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagttctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtggcccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggtaggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaaccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtctgactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagtttgaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaagaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggccgcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacctggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttttgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctccatccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctccctttatccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgccacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgaccttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtgcctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggctccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccgactctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctaccggactcagatctaAGCTTGCCACCATGGATAAAGTTTTAAACAGAGAGGAATCTTTGCAGCTAATGGACCTTCTAGGTCTTGAAAGGAGTGCCTGGGGGAATATTCCTCTGATGAGAAAGGCATATTTAAAAAAATGCAAGGAGTTTCATCCTGATAAAGGAGGAGATGAAGAAAAAATGAAGAAAATGAATACTCTGTACAAGAAAATGGAAGATGGAGTAAAATATGCTCATCAACCTGACTTTGGAGGCTTCTGGGATGCAACTGAGATTCCAACCTATGGAACTGATGAATGGGAGCAGTGGTGGAATGCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGATGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAGAAGAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGCTAAGTTTTTTGAGTCATGCTGTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACACCACAAAGGAAAAAGCTGCACTGCTATACAAGAAAATTATGGAAAAATATTCTGTAACCTTTATAAGTAGGCATAACAGTTATAATCATAACATACTGTTTTTTCTTACTCCACACAGGCATAGAGTGTCTGCTATTAATAACTATGCTCAAAAATTGTGTACCTTTAGCTTTTTAATTTGTAAAGGGGTTAATAAGGAATATTTGATGTATAGTGCCTTGACTAGAGATCCATTTTCTGTTATTGAGGAAAGTTTGCCAGGTGGGTTAAAGGAGCATGATTTTAATCCAGAAGAAGCAGAGGAAACTAAACAAGTGTCCTGGAAGCTTGTAACAGAGTATGCAATGGAAACAAAATGTGATGATGTGTTGTTATTGCTTGGGATGTACTTGGAATTTCAGTACAGTTTTGAAATGTGTTTAAAATGTATTAAAAAAGAACAGCCCAGCCACTATAAGTACCATGAAAAGCATTATGCAAATGCTGCTATATTTGCTGACAGCAAAAACCAAAAAACCATATGCCAACAGGCTGTTGATACTGTTTTAGCTAAAAAGCGGGTTGATAGCCTACAATTAACTAGAGAACAAATGTTAACAAACAGATTTAATGATCTTTTGGATAGGATGGATATAATGTTTGGTTCTACAGGCTCTGCTGACATAGAAGAATGGATGGCTGGAGTTGCTTGGCTACACTGTTTGTTGCCCAAAATGGATTCAGTGGTGTATGACTTTTTAAAATGCATGGTGTACAACATTCCTAAAAAAAGATACTGGCTGTTTAAAGGACCAATTGATAGTGGTAAAACTACATTAGCAGCTGCTTTGCTTGAATTATGTGGGGGGAAAGCTTTAAATGTTAATTTGCCCTTGGACAGGCTGAACTTTGAGCTAGGAGTAGCTATTGACCAGTTTTTAGTAGTTTTTGAGGATGTAAAGGGCACTGGAGGGGAGTCCAGAGATTTGCCTTCAGGTCAGGGAATTAATAACCTGGACAATTTAAGGGATTATTTGGATGGCAGTGTTAAGGTAAACTTAGAAAAGAAACACCTAAATAAAAGAACTCAAATATTTCCCCCTGGAATAGTCACCATGAATGAGTACAGTGTGCCTAAAACACTGCAGGCCAGATTTGTAAAACAAATAGATTTTAGGCCCAAAGATTATTTAAAGCATTGCCTGGAACGCAGTGAGTTTTTGTTAGAAAAGAGAATAATTCAAAGTGGCATTGCTTTGCTTCTTATGTTAATTTGGTACAGACCTGTGGCTGAGTTTGCTCAAAGTATTCAGAGCAGAATTGTGGAGTGGAAAGAGAGATTGGACAAAGAGTTTAGTTTGTCAGTGTATCAAAAAATGAAGTTTAATGTGGCTATGGGAATTGGAGTTTTAGATTGGCTAAGAAACAGTGATGATGATGATGAAGACAGCCAGGAAAATGCTGATAAAAATGAAGATGGTGGGGAGAAGAACATGGAAGACTCAGGGCATGAAACAGGCATTGATTCACAGTCCCAAGGCTCATTTCAGGCCCCTCAGTCCTCACAGTCTGTTCATGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAGCggccgcctcggccaaacatcgataaaataaaagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggcatggaaaaatacataactgagaatagagaagttcagatcaaggtcaggaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggggcgccagtcctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatttgggggctcgtccgggatcgggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgtactgataacttcgtataatgtatgctatacgaagttattaggtctgaagaggagtttacgtccagccaagctagggccgcgatccggaacccttaatataacttcgtataatgtatgctatacgaagttatcagtactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattgctagcaattgctagcaattgctagcaattcataccagatcaccgaaaactgtcctccaaatgtgtccccctcacactcccaaattcgcgggcttctgcctcttagaccactctaccctattccccacactcaccggagccaaagccgcggcccttccgtttctttgct
LOCUS dna 8358 bp
FEATURES Location/Qualifiers
Other Gene 659..1468
/gene="psi_plus_pack other"
ORF 1449..2216
/sequence="ORF_3 rf(5)"
Marker 1566..2165
/gene="puro marker"
misc_binding 1674..1679
/dbxref="REBASE:AccI"
misc_binding 1895..1900
/dbxref="REBASE:StuI"
Promoter 2787..2868
/gene="CMV prom"
misc_binding 2888..2893
/dbxref="REBASE:BglII"
ORF 2906..5032
/sequence="ORF_1 rf(2)"
Other Gene 3164..5032
/gene="SV40_lrgT other"
misc_binding 3985..3990
/dbxref="REBASE:HpaI"
misc_binding 4948..4953
/dbxref="REBASE:BclI"
misc_binding 5033..5040
/dbxref="REBASE:NotI"
misc_binding 5035..5047
/dbxref="REBASE:SfiI"
misc_binding 5052..5057
/dbxref="REBASE:ClaI"
Other Gene 5139..5690
/gene="5_LTR2 other"
misc_binding 5394..5399
/dbxref="REBASE:XbaI"
Other Gene 5943..5976
/gene="loxP other"
Other Gene 6036..6069
/gene="loxP other"
Rep_Origin 6345..6964
/gene="pBR322 origin"
Marker 7119..7979
/gene="amp marker"
ORF 7119..7979
/sequence="ORF_2 rf(5)"
Promoter 8021..8049
/gene="amp prom"
BASE COUNT 2144 a 2123 c 2071 g 2020 t 0 others