Custom CRISPR sgRNA Lentiviral Vectors & Viruses
abm carries a variety of CRISPR lentivectors and recombinant lentiviruses that will streamline your CRISPR gene editing experiments, including gene knockout, activation, repression and epigenetic modifications. Choose from our All-in-One system or our sgRNA only expression system and pair the latter with a Cas9-Expressing Stable Cell Lines or spCas9 Lentivirus. Inquire with us below to get started on your custom CRISPR project.
Advantages of the Lentivirus System:
- High infection efficiency
- Stable integration & expression of recombinant proteins
- Large insert capacity (up to 5kb)
- Ideal system for the creation of stable cell lines
- Broad host range: infection of dividing, non-dividing, stem cells, and primary cells
Service Details
Cloning Services
| Service | Vector | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – single target | pLenti-U6-sgRNA-PGK-Neo | 1.0 μg | C437 | |
| Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – set of three targets | pLenti-U6-sgRNA-PGK-Neo | 3 x 1.0 μg | C441 | |
| Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – single target | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro | 1.0 μg | C438 | |
| Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – set of three targets | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro | 3 x 1.0 μg | C442 | |
| Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – single target | pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP | 1.0 μg | C515 | |
| Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a) – set of three targets | pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP | 3 x 1.0 μg | C516 | |
| Custom CRISPR All-in-One sgRNA Lentiviral Vector (with AaCas12b) – set of three targets | pLenti-U6-sgRNAaa-SFFV-AaCas12b-2A-Puro | 3 x 1.0 μg | G6102 | |
| Custom CRISPR sgRNA only Lentiviral Vector (with AaCas12b) – set of three targets | pLenti-U6-sgRNAaa-PGK-Neo | 3 x 1.0 μg | G6104 |
Lentivirus Packaging Services
| Service | Unit | Titer | Purity | Cat. No. | Lead Time | Price |
|---|---|---|---|---|---|---|
| Custom CRISPR Lentivirus Packaging – single target | 3 x 250 μl | 108 IU/ml | Research grade | C445 | 2-3 weeks | |
| Custom CRISPR Lentivirus Packaging – three targets, individually packaged | 3 x 750 µl | 108 IU/ml | Research grade | C447 | 2-3 weeks | |
| Custom CRISPR Lentivirus Packaging – three targets, pooled packaging | 3 x 250 μl | 108 IU/ml | Research grade | C448 | 2-3 weeks |
*For additional titer or volume options, please inquire.
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector/Virus | Lentivector/Virus | Non-Viral Vector | Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| All-in-One with AaCas12b | - | Available | - | - |
| sgRNA for AaCas12b | Available | Available | - | - |
| Browse Collection |
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Additional Resources
Documents
FAQs
| What promoter(s) do your sgRNA and All-in-One lentivectors have? |
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sgRNAs are expressed from the U6 promoter and Cas9 is expressed from the SFFV promoter.
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| What is the approximate time frame to generate CRISPR lentiviral constructs? |
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The typical lead time is around 2-3 weeks for CRISPR lentivectors and 2-3 weeks for lentiviruses. Please inquire for a more accurate lead time.
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| How should I store my lentivirus? |
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Aliquots should be made for the lentivirus and stored at -80°C.
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| If a high-titer custom lentivirus was ordered, is the lentiviral titration performed after a freeze-thaw or immediately after production before the freeze-down step? |
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We do the titration after aliquoting and freeze-down. Thus, the titer should be accurate for the customer after they thaw the finished product for the first time.
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| Which virus has your lentivirus expression system been derived from? Is it HIV? |
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Our lentivirus expression system is derived from Human HIV-1 Virus. It employs third generation self-inactivating recombinant lentiviral vectors with enhanced biosafety features and minimal relation to wild-type Human HIV-1 Virus.
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| How do you verify the titer? |
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We use our LV900 series - qPCR Lentivirus Titration Kit. This kit quantifies a proprietary region of the lentiviral 5’-LTR.
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| What is the packaging capacity for Lentivirus? |
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The maximum insert size is <9 kb between the 5'LTR to 3'LTR.
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Citations
| 01 | Fleck JS, et al. "Inferring and perturbing cell fate regulomes in human brain organoids." Nature (2022). doi: 10.1038/s41586-022-05279-8 |
| 02 | Das A, et al. "Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus." Nature Microbiology (2020). doi: 10.1038/s41564-020-0727-8 |
| 03 | Yamane D, et al. "Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses." Nature Microbiology (2019). doi: 10.1038/s41564-019-0425-6 |