Custom CRISPR sgRNA Non-Viral Vectors
abm offers Custom All-in-One (Cas9 and sgRNA expression) and sgRNA-only expressing Non-Viral Vectors that simplify and streamline your CRISPR/Cas9 experiment. Choose from our All-in-One system or our sgRNA-only expression system, and pair the latter with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease. Inquire with us below to get started on your custom CRISPR project.
A non-viral vector from abm is the simplest approach to deliver sgRNAs into cells—ideal for transient sgRNA expression to minimize off-target effects. Once the non-viral vector has done its work, it can be diluted out of the cell population by passaging the cells and monitoring the GFP fluorescence until it disappears.
Service Details
| Service | Vector | Unit | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – single target | pNV-sgRNA-GFP | 1.0 µg | C439 | |
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – set of three targets | pNV-sgRNA-GFP | 3 x 1.0 µg | C443 | |
| Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – single target | pNV-sgRNA-Cas9-2A-GFP | 1.0 µg | C440 | |
| Custom CRISPR All-in-One Non-Viral Vector(with spCas9) – set of three targets | pNV-sgRNA-Cas9-2A-GFP | 3 x 1.0 µg | C444 |
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector/Virus | Lentivector/Virus | Non-Viral Vector | Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| All-in-One with AaCas12b | - | Available | - | - |
| sgRNA for AaCas12b | Available | Available | - | - |
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Additional Resources
Documents
Citations
| 01 | Fleck JS, et al. "Inferring and perturbing cell fate regulomes in human brain organoids." Nature (2022). doi: 10.1038/s41586-022-05279-8 |
| 02 | Das A, et al. "Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus." Nature Microbiology (2020). doi: 10.1038/s41564-020-0727-8 |
| 03 | Yamane D, et al. "Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses." Nature Microbiology (2019). doi: 10.1038/s41564-019-0425-6 |