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Recombinant AAV Vectors
As a leader in recombinant AAV technology, abm has developed a variety of AAV expression systems which can be used to express your gene of interest within a broad range of host cells utilizing the natural tissue tropism of different serotypes. To facilitate the application and versatility of our AAV vectors, this expression system is available to purchase for many applications with a variety of genetic elements. Can't find the AAV that you're looking for? Contact our team for a custom AAV or browse our other ready-to-use gene libraries.
| Cloning Vector | Cat. No. | Reporter | Price |
|---|---|---|---|
| CRISPR | |||
| pAAV-PGK-saCas9-U6-sgRNAsa | C307 | ||
| pAAV-U6-sgRNAsa-GFP | C305 | GFP | |
| pAAV-U6-sgRNAsp-GFP | C303 | GFP | |
| cDNA expression | |||
| pAAV-G-hSyn | C234 | - | |
| pAAV-G-EF1a | C234 | - | |
| pAAV-G-PGK | C234 | - | |
| pAAV-G-CAGGS | C234 | - | |
Choosing the right promoter
| Promoter | Applications |
|---|---|
| CMV (Strong) | A widely used promoter that provides strong gene expression in most cells (weak activity in human and mouse stem cells). |
| EF1α | Strong expression promoter; highly efficient in stem cells. Good for stable expression from long term cultures. |
| PGK (Medium) | A high efficiency promoter able to sustain stable activity in long term cultures of undifferentiated stem cells. |
| CAGGS (Strong) | A large hybrid promoter (composed of the CMV immediate-early enhancer as well as the CBA and CBA intron 1/exon 1 promoter) with no methylation issues (max.insert size:2kb). |
| hSyn (Medium-strong) | The synapsin I promoter; has been used to acheive highly neuron-specific long-term transgene expression in vivo. |