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TM076 | Research Use Only (RUO)

3DCelMatrix™ ECM Solution for Organoid, Spheroid, and 3D Cell Culture

Ready-to-use extracellular matrix consistency for reproducible 3D cell culture workflows

3DCelMatrix™ is a ready-to-use extracellular matrix solution optimized for consistent organoid and spheroid culture across oncology, stem cell, primary cell, and drug screening applications.

Designed to minimize variability and simplify handling, 3DCelMatrix™ helps researchers generate robust, reproducible 3D culture results with confidence.

3DCelMatrix™ ECM Solution Product Highlights

Every feature of 3DCelMatrix™ is built around practical 3D culture needs: reproducibility, simplicity, workflow flexibility, and consistent morphology.

Reduced Variability

Strict Lot-to-Lot QC

Minimizes failed experiments and improves reproducibility across repeated 3D culture workflows.

Simplified Handling

Ready-to-Use Workflow

Reduces preparation time from days to minutes and simplifies bench handling.

Flexible ECM Compatibility

Thin Gel, Thick Gel, and Coating

Supports thin gel, thick gel, and coating applications across diverse 3D cell culture needs.

Consistent Organoid Formation

Robust 3D Morphology

Optimized to support spheroid and organoid morphology across stem cell and primary cell applications.

Flexible ECM Workflows for Thin Gel, Thick Gel, and Coating Applications

Use 3DCelMatrix™ across invasion, migration, organoid embedding, spheroid embedding, and surface-coating workflows.

Thin gel ECM workflow for invasion and migration assays using 3DCelMatrix
Thin Gel

1–2 mg/mL

Invasion & Migration

Use lower concentrations for invasion and migration studies.

Thick gel ECM workflow for organoid and spheroid embedding using 3DCelMatrix
Thick Gel

3–5 mg/mL

Organoid / Spheroid Embedding

Supports embedding workflows for spheroids and organoids.

Coating ECM workflow for surface coating applications using 3DCelMatrix
Coating

0.5–2% v/v

Surface Coating

Ideal for coating culture surfaces and related applications.

Simple 5-Step 3D Cell Culture Workflow

A streamlined workflow helps researchers move from thawing to culture with fewer handling steps.

Thaw 3DCelMatrix ECM solution on ice before 3D cell culture
Step 1

Thaw

Thaw 3DCelMatrix™ at 2–8°C on ice. Keep tubes, pipette tips, and plates pre-chilled.

Prepare 3DCelMatrix by diluting with cold media or PBS
Step 2

Prepare

Dilute to the desired concentration using cold media or PBS.

Apply 3DCelMatrix into pre-chilled culture plates
Step 3

Apply

Transfer quickly into pre-chilled culture plates or vessels to prevent premature gelation.

Gelation of 3DCelMatrix at 37 degrees Celsius
Step 4

Gelation

Incubate at 37°C for 15–30 minutes until gel formation occurs.

Seed and culture cells with 3DCelMatrix ECM solution
Step 5

Seed & Culture

Seed cells on or within the matrix and maintain under standard culture conditions.

3D Cell Culture Applications

3DCelMatrix™ supports a broad range of 3D biology workflows across academic research, translational science, and drug discovery.

Organoid Culture

Supports robust organoid formation for intestinal, kidney, lung, and brain organoid systems.

Tumor Spheroid Formation

Enables physiologically relevant 3D tumor models for oncology and therapeutic screening workflows.

Drug Screening & Disease Modeling

Provides consistent 3D culture conditions for predictive drug response and disease research.

Stem Cell & Primary Cell Expansion

Supports scalable growth of stem cells and primary cells in biologically relevant 3D environments.

Cell Invasion & Migration Assays

Ideal for studying extracellular matrix interactions and cellular behavior in 3D systems.

3DCelMatrix™ Technical Specifications

Attribute Specification
Catalog Number TM076
Product Name 3DCelMatrix™ ECM Solution
Volume 10 mL
Source EHS tumor-derived
Formulation LDEV-free; Defined ECM
Storage −20°C
Gelation Time <30 minutes
Protein Concentration >8 mg/mL
Endotoxin <4.0 EU/mL
Sterility Negative
Regulatory Status Research Use Only (RUO)
Lead Time 2–3 business days
3DCelMatrix ECM solution vial for organoid and spheroid culture
Ready-to-use ECMDesigned for organoid, spheroid, and 3D culture workflows.
Research Use OnlyFor laboratory research applications.
Defined workflow supportCompatible with thin gel, thick gel, and coating use cases.

Representative Organoid, Spheroid, and iPSC Culture Results

3DCelMatrix™ supports human kidney organoids, human midbrain organoids, iPSC colony culture, complex lumen-like organoid morphology, and uniform spheroid formation.

Click images to enlarge.

Organoid Analysis Overview montage.
iPSC Colony Representative colony culture.
Spheroid Formation Uniform morphology.
Midbrain Organoid Complex morphology.
Kidney Organoid Representative culture.

Frequently Asked Questions About 3DCelMatrix™

What is 3DCelMatrix™ used for?

3DCelMatrix™ is used as an extracellular matrix solution for organoid culture, spheroid formation, stem cell and primary cell expansion.

Is 3DCelMatrix™ suitable for organoid and spheroid culture?

Yes. 3DCelMatrix™ supports organoid and spheroid workflows, including thick gel embedding, thin gel applications, and surface coating.

Can 3DCelMatrix™ be used as a Matrigel alternative?

3DCelMatrix™ is designed for researchers looking for a ready-to-use ECM solution for 3D cell culture workflows, including applications commonly associated with basement membrane matrix products such as Matrigel, Geltrex, and Cultrex.

What workflows does 3DCelMatrix™ support?

3DCelMatrix™ supports thin gel workflows for invasion and migration, thick gel workflows for organoid and spheroid embedding, and coating workflows for surface preparation.

Is 3DCelMatrix™ for clinical or research use?

3DCelMatrix™ is intended for Research Use Only.

Which products pair with 3DCelMatrix™?

3DCelMatrix™ can be paired with SpheroWell™ culture products and organoid media for spheroid, organoid, and 3D culture workflows.

The abm Difference

More than a supplier, abm is your research partner — delivering trusted quality, rapid access, and expert support from project start to publication.

Reliable Supply

Consistent availability helps reduce downtime and keep 3D culture workflows moving.

Scientist-to-Scientist Support

Our technical team can help identify compatible workflows, culture conditions, and related products.

Complete Documentation

Access product information and supporting documentation for streamlined lab integration.

3D Culture Ecosystem

Combine ECM, ULA formats, and organoid media to support broader 3D model development.

Ready to Evaluate 3DCelMatrix™?

Apply for early adopter pricing, view the full product details, or connect with our team to discuss your 3D culture workflow.

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Tell us a little about your research workflow and how you plan to evaluate 3DCelMatrix™. A member of the abm team will review your submission and contact you about early adopter pricing, availability, and next steps.