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siRNA dsRNA Oligo Library
siRNAs are potent gene-silencing tools that can be delivered via double-stranded RNA (dsRNA) for rapid, transient knockdown without modifying the host genome. Suitable for both in vitro and in vivo applications, abm provides pre-designed sets of three siRNA oligo duplexes targeting any human, mouse, or rat gene, along with effective controls. These duplexes can be transfected individually or pooled for enhanced knockdown efficiency.
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Key Features:
- Produced under ISO9000 standards.
- HPLC purified >97%.
- Chemically modified (2’-OMe) for increased stability and lower toxicity.
- 19-23 bases for each duplex in lyophilized format.
- Set of 3 siRNA oligo duplexes for enhanced gene knockdown.
- Included positive and negative controls.
- Guaranteed knockdown of at least 70% in every set.
Additional Information
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Mechanism
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Workflow
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siRNA Oligo User Instructions
Click to download.
FAQs
| What are siRNA dsRNA oligos? |
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siRNA dsRNA oligos are short, chemically synthesized double-stranded RNA molecules designed to specifically target and degrade complementary mRNA, resulting in transient gene silencing.
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| How do synthetic siRNA oligos differ from vector-based RNAi? |
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Synthetic siRNA oligos are delivered directly into cells and produce rapid, transient knockdown, whereas vector-based RNAi systems rely on intracellular expression and typically provide longer-lasting gene silencing.
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| What delivery methods are commonly used for siRNA oligos? |
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siRNA dsRNA oligos are most commonly delivered using lipid-based or polymer-based transfection reagents. Electroporation may be used for difficult-to-transfect or primary cells.
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| How long does gene silencing from siRNA oligos last? |
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Knockdown from synthetic siRNA oligos usually begins within 24-48 hours after transfection and lasts several days, depending on cell type, target gene, and experimental conditions.
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