Custom Cre-Inducible Gene Expression (DIO) Vectors
Use abm’s double-floxed inverse ORF (DIO) vectors for Cre-inducible expression of your gene-of interest! The DIO system allows you to express your target gene only in cells/tissues that also express the Cre recombinase protein, allowing for tissue-specific gene expression. This system is also called Cre-ON, Cre-Switch, FLEx-Cre, Flex-rev (reverse), or Flex-ON. We offer high-titer viral packaging services (lentivirus or AAV) as well as Cre-expression AAVs and tamoxifen-inducible Cre Lentivirus to support your DIO project from cloning to expression.
Service Details
Core Services
| Service | Unit | Cat.No. | Price |
|---|---|---|---|
| Gene Synthesis | Per bp | C098 | from /bp |
| Vector Backbone for Cloning Lentivector Options: pLenti-GIII-CMV-DIO-SV40-Puro pLenti-GIII-CMV-DIO-SV40-copGFP AAV Options: pAAV-G-CMV-DIO pAAV-G-CMV-DIO-SV40-Puro pAAV-G-CMV-DIO-SV40-copGFP |
Not a Deliverable | C318 | |
| Subcloning Service I | 1.0 μg | C096 | |
| Promoter Change Service | 1.0 μg | C126 | |
| Tag Addition/Removal Service (≤30bp) | 1.0 μg | C127 |
Controls
| Product | Unit | Cat.No. | Price |
|---|---|---|---|
| pLenti-CMV-DIO-GFP-SV40-Puro | 1.0 μg | LV661 | |
| pLenti-CMV-DIO-Blank-SV40-Puro | 1.0 μg | LV662 | |
| pLenti-CMV-DIO-Blank-SV40-GFP | 1.0 μg | LV663 | |
| pAAV-CMV-DIO-GFP | 1.0 μg | AAV101 | |
| pAAV-CMV-DIO-GFP-SV40-Puro | 1.0 μg | AAV102 | |
| pAAV-CMV-DIO-Blank-SV40-Puro | 1.0 μg | AAV103 | |
| pAAV-CMV-DIO-Blank-SV40-GFP | 1.0 μg | AAV104 |
Mechanism
Supporting Data
Lentivirus:
Left: CRE expressing 293 cells infected with Cat. No. LV661 results in copGFP expression (presence of CRE)
Right: 293 cells infected with Cat. No. LV661 results in no copGFP expression (absence of CRE)
AAV:
Left: CRE expressing 293 cells infected with Cat. No. AAV101 results in copGFP expression (presence of CRE)
Right: 293 cells infected with Cat. No. AAV101 results in no copGFP expression (absence of CRE)
