Custom CRISPR sgRNA Non-Viral Vectors

abm offers Custom All-in-One (Cas9 and sgRNA expressing) and sgRNA only expressing Non-Viral Vectors that will simplify and streamline your CRISPR/Cas9 experiment. Choose from our All-in-One system or our sgRNA only expression system and pair the latter with a Cas9-Expressing Stable Cell Line or spCas9 Nuclease. Just tell us what you want to accomplish and we'll do the sgRNA design and cloning for you. 

A non-viral vector from abm is the simplest approach to deliver sgRNAs into cells, ideal for expressing sgRNA in a transient manner to limit off-target effects. When the non-viral vector has done its work, it can be diluted out of the cell population by passaging; just monitor the GFP fluorescence signal until it is gone.

Available Custom CRISPR Vectors and Viruses:
AAV Vector
sgRNA for spCas9 Available Available Available Available
sgRNA for saCas9 Available - - -
All-in-One with spCas9 - Available Available -
All-in-One with saCas9 Available - - -
All-in-One with FnCas12a (FnCpf1) - Available - -
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"Construct works beautifully, definitely will get more if needed."

Emily Chen, UCLA, CRISPR Constructs

Additional Resources:

Service Details

Service Name Vector Quantity Cat. No. Price
Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – single target pNV-sgRNA-GFP 1.0 ug C439 $135.00
Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – set of three targets pNV-sgRNA-GFP 3 x 1.0 ug C443 $360.00
Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – single target pNV-sgRNA-Cas9-2A-GFP 1.0 ug C440 $125.00
Custom CRISPR All-in-One Non-Viral Vector(with spCas9) – set of three targets pNV-sgRNA-Cas9-2A-GFP 3 x 1.0 ug C444 $355.00

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03 Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359