Custom CRISPR sgRNA Lentiviral Vectors & Viruses

abm carries a variety of CRISPR lentivectors and lentiviruses that will streamline your CRISPR gene editing experiments, including gene knockout, activation, repression and epigenetic modifications. Choose from our All-in-One system or our sgRNA only expression system and pair the latter with a Cas9-Expressing Stable Cell Lines or spCas9 Nuclease. Just tell us what you want to accomplish and we'll do the sgRNA design, cloning and viral packaging for you!

The lentivirus offers several advantages including: the ability to infect both dividing and non-dividing cells in vitro and in vivo and allows for stable integration into the host genome resulting in long-term construct expression even in difficult to infect cell lines. Lentivectors on the other hand, can be transfected into the desired cell line resulting in transient expression which can later be diluted out.

Other Available CRISPR Lentivirus Systems:

•Gene Knockout using spCas9
•Gene Activation using dCas9-VPR
•Gene Repression using dCas9-KRAB
•Epigenetic Gene Repression using dCas9-DNMT3A
•Epigenetic Gene Activation using dCas9-TET1CD

Available Custom CRISPR Vectors and Viruses:
AAV Vector
/Virus
Lentivector
/Virus
Non-Viral
Vector
Adenovirus
sgRNA for spCas9 Available Available Available Available
sgRNA for saCas9 Available - - -
All-in-One with spCas9 - Available Available -
All-in-One with saCas9 Available - - -
All-in-One with FnCas12a (FnCpf1) - Available - -
Browse Collection Browse Collection Browse Collection Browse Collection



Additional Resources:

"I have used abm's All-in-One lentiviral system to knockout human IL11, which worked really well. Our labmate also ordered the same system from abm to knockout mouse Axl, and she got good results, too. Thus, we want to stick to the same system."

Dr. Ejung Moon, Stanford University, CRISPR

Service Details

Cloning Services
Service Name Vector Quantity Cat. No. Price
Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – single target pLenti-U6-sgRNA-PGK-Neo 1.0 μg C437 $95.00
Custom CRISPR sgRNA Lentiviral Vector (for spCas9) – set of three targets pLenti-U6-sgRNA-PGK-Neo 3 x 1.0 μg C441 $250.00
Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – single target pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro 1.0 μg C438 $105.00
Custom CRISPR All-in-One Lentiviral Vector (with spCas9) – set of three targets pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro 3 x 1.0 μg C442 $280.00
Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a / FnCpf1) – Single Target pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP 1.0 μg C515 $195.00
Custom CRISPR All-in-One Lentiviral Vector (with FnCas12a / FnCpf1) – Set of Three Targets pLenti-U6-sgRNA-EF1a-FnCas12a-2A-GFP 3 x 1.0 μg C516 $465.00
GFP/RFP Reporter Switch Service 1 Service C129 $350.00
Packaging Service - Standard Size Titer CAT. NO. PRICE
Custom CRISPR Lentivirus Packaging – single target 4 x 500 μl 107 IU/ml C445 $395.00
Custom CRISPR Lentivirus Packaging – three targets, individually packaged 3 x 2 ml 107 IU/ml C447 $940.00
Custom CRISPR Lentivirus Packaging – three targets, pooled packaging 4 x 500 μl 107 IU/ml C448 $395.00

*For additional titer or volume options, please inquire.

Additional Info

Workflow

FAQs

What promoter(s) do your sgRNA and All-in-One lentivectors have?
sgRNAs are expressed from the U6 promoter and All-in-One lentivectors and lentiviruses express Cas9 from the SFFV promoter.
What is the approximate time frame to generate CRISPR lentiviral constructs?
The typical lead time is around 1-3 weeks for CRISPR lentivectors and 4-6 weeks for lentiviruses. Please inquire for a more accurate lead time.
How should I store my lentivirus?
Aliquots should be made for the lentivirus and stored at -70 degrees Celsius.
If a high-titer custom lentivirus was ordered, do you do the lentiviral titration after a freeze-thaw or straight after production before the freeze-down step?
We do the titration after aliquoting and freeze-down. Thus, the titer should be accurate for the customer after they thaw the finished product for the first time.
Which virus has your lentivirus expression system been derived from? Is it HIV?
Our lentivirus expression system is derived from Human HIV-1 Virus. It employs third generation self-inactivating recombinant lentiviral vectors with enhanced biosafety features and minimal relation to wild-type Human HIV-1 Virus.
How do you verify the titer?
We use our LV900 series - qPCR Lentivirus Titration(Titer) Kit. This kit quantifies a proprietary region of the lentiviral 5’-LTR.
What is the packaging capacity for Lentivirus?
The maximum insert size is <9 kb between the 5'LTR to 3'LTR.

Citations

01 Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371
02 Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338
03 Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359