Custom protein production in E. coli
Escherichia coli (E. coli) provides for a relatively simple, fast, inexpensive and robust system for native and heterologous recombinant protein production. While unable to render many of the complex eukaryotic post-translational modifications, technologies such as, co-expression of chaperones/kinases/rare tRNAs and engineering of strains that provide a pro-disulfide bond (oxidative) environment, hallmark the technological progress achieved in E. coli system over the last two decades. abm recommends the E. coli system when post-translational modifications such as glycosylation are not crucial to the intended downstream application(s).
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Custom protein production in Mammalian cells
The mammalian expression system provides the only authentic and feasible option in cases where the protein of interest needs to be embellished with characteristic, mammalian post-translational modification(s) and/or entails a complex translational machinery for proper folding and targeting (such as membrane proteins). Therefore, abm recommends its mammalian expression service for proteins for which the native folding and post-translational modifications are of a complex nature (beyond the purview of E. coli ) and indispensable to the customer's intended application (s).
Please note that only the recombinant protein is delivered when Phase I ---> IV are ordered. If you would like to receive the product resulting from each phase, you may do so at an extra charge:
Phase II: Stable protein producing cell-line- polyclonal (293 cells) @ $2000
Phase III: 500ul of crude lysate (in WB sample buffer, denatured) @ $100
You may choose to stop at any phase, however please note that handling charges apply if you wish to receive the deliverables from a specific Phase.
A non-refundable deposit applies to initiate the project, regardless of how many phases were selected for the service.
If you are unable to provide the insert, we can provide an additional gene synthesis service. Please provide the sequence for this additional service.