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Recombinant Lentiviral Vectors

As a world leader in lentiviral technology, abm has developed a variety of recombinant lentiviral expression systems which can be used to express your gene of interest within a broad range of host cells. To facilitate the application and versatility of our lentiviral vectors, this expression system is available to purchase in many combinations of different promoters, tag sequences and reporter genes, and even multicistronic vectors allowing for multiple gene expression from one single construct.


Cloning Vector Cat. No. Application Promoter Tag/Reporter Marker
CRISPR
pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro Search All-in-One sgRNA/Cas9 U6   Puro
pLenti-U6-sgRNA-PGK-Neo Search sgRNA U6   Neo
pLenti-Multi-gRNA-PGK-Neo Service Multiple sgRNA U6   Neo
pLenti-EF1a-dCas9-Cloning K014 dCas9 Cloning Vector EF1a   Puro
cDNA Expression
pLenti-Easy-His LV009 cDNA expression CMV His Neo
pLenti-Easy-HA LV005 cDNA expression CMV His Neo
pLenti-III-HA LV022 cDNA expression CMV His Puro
pLenti-III-UbC G300 cDNA expression UbC   Puro
pLenti-III-PGK G305 cDNA expression PGK   Puro
pLenti-III-EF1a LV043 cDNA expression EF1a   Puro
Fluorescent Reporters
pLenti-III-2A-GFP LV070 cDNA-2A-GFP CMV GFP Puro
pLenti-CMV-GFP-2A-Puro LV073 cDNA, GFP-2A-Puro CMV GFP Puro
pLenti-EF1a-GFP-2A-Puro LV067 cDNA, GFP-2A-Puro EF1a GFP Puro
pLenti-CMV-RFP-2A-Puro LV084 cDNA, RFP-2A-Puro CMV RFP Puro
Promoterless
pLenti-III-promoterless LV013 Promoterless     Puro
pLenti-III-promoterless-GFP LV059 Promoterless   GFP Puro
pLenti-III-promoterless-Luc LV092 Promoterless   Luc Puro
siRNA
piLenti-siRNA-GFP (BbsI Linearized) LV016 siRNA U6, H1 GFP Puro
piLenti-siRNA (BbsI Linearized) LV014 siRNA U6, H1   Puro
miRNA
pLenti-III-mir-GFP m016 miRNA CMV GFP Puro
pLenti-III-UTR-Dual-Luc m030 3'UTR reporter CMV Luc/Ren Luc Puro
pLenti-III-mir-OFF Search miRNA inhibitor H1 GFP Puro

Choosing the right promoter


Promoter Applications
CMV The most widely used promoter. Very strong gene expression promoter in most cellular systems; weak activity in human and mouse stem cells.
EF1a Strong expression promoter; highly efficient in stem cells. Good for stable expression from long term cultures.
PGK Medium expression promoter. Highest efficiency in stem cells; Sustains stable activity in long term cultures of undifferentiated stem cells.
UbC Relatively low expression promoter. Sustains stable expression.

Try our Lentivirus Promoter Blast™ kit to find the best promoter for your research applications.


Tag: The CMV promoter vectors are offered with His or HA Tag. The addition of a tag can help you detect the protein expressed by western blot or IHC, or facilitate downstream protein purification if needed.


Reporters: abm offers both GFP and RFP in Fusion (in frame) with your gene of interest and in Bicistronic (driven by a separate promoter) formats.