Cell Immortalization Kits
Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.
Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience, abm has developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.
|Gene Overexpressed||Viruses Offered||Application|
|Reliable and commonly used.|
|SV40 T Antigen||Lentivirus||Reliable and commonly used. Often used to immortalize epithelial cells.|
|HPV E6-E7||Lentivirus||Immortalize keratinocytes.|
|HOXB8, HOXA9, HOXA10||Lentivirus||Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells.|
|E1A-E1B||Lentivirus||Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells.|
|p53 siRNA, Rb siRNA||Lentivirus||Co-express with hTERT for better immortalization of epithelial cells.|
|Bmi1||Lentivirus||Immortalize epithelial cells.|
|CDK4||Lentivirus||Co-express with hTERT for a more authentic cell model.|
|Myc T58A (c-Myc), Ras V12 (H-Ras)||Lentivirus||Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.).|
Popular Products Additional Resources on Cell Culture Methods