Cell Immortalization Kits

Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience, abm has developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.

Gene Overexpressed Viruses Offered Application
HTERT Adenovirus,
Reliable and commonly used.
SV40 T Antigen Lentivirus Reliable and commonly used. Often used to immortalize epithelial cells.
HPV E6-E7 Lentivirus Immortalize keratinocytes.
HOXB8, HOXA9, HOXA10 Lentivirus Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells.
E1A-E1B Lentivirus Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells.
p53 siRNA, Rb siRNA Lentivirus Co-express with hTERT for better immortalization of epithelial cells.
Bmi1 Lentivirus Immortalize epithelial cells.
CDK4 Lentivirus Co-express with hTERT for a more authentic cell model.
Myc T58A (c-Myc), Ras V12 (H-Ras) Lentivirus Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.).

Tools & Resources

  • Cell Immortalization Handbook
    Cell Immortalization Handbook
    This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.
  • Cell Immortalization Workflow
    Cell Immortalization Workflow
    Overview of abm's Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.
  • Custom Cell Immortalization Service
    Custom Cell Immortalization Service
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