Cell Immortalization Kits

Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.

Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience,abmhas developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.

Gene OverexpressedViruses OfferedApplication
hTERT Adenovirus,
Lentivirus,
Retrovirus
Reliable and commonly used.
SV40 T Antigen Adenovirus,
Lentivirus,
Retrovirus
Reliable and commonly used. Often used to immortalize epithelial cells.
EBV genes Epstein-Barr Virus Immortalize B lymphocytes, and sometimes T lymphocytes.
HPV E6-E7 Lentivirus Immortalize keratinocytes.
HOXB8, HOXA9, HOXA10 Lentivirus Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells.
Adenoviral E1A-E1B Lentivirus Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells.
p53 siRNA, Rb siRNA Lentivirus Co-express with hTERT for better immortalization of epithelial cells.
Bmi1 Lentivirus Immortalize epithelial cells.
CDK4 Lentivirus Co-express with hTERT for a more authentic cell model.
Myc T58A (c-Myc), Ras V12 (H-Ras) Lentivirus Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.).

Tools & Resources

  • Cell Immortalization Handbook
    Cell Immortalization Handbook
    This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.
  • Cell Immortalization Workflow
    Cell Immortalization Workflow
    Overview of abm's Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.
  • Custom Cell Immortalization Service
    Custom Cell Immortalization Service
    Can't find what you are looking for? We can help!

Browse Products

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  1. Sku: LV675
    Price: $1,450.00
    Unit: 5 x 20 µl
    High Titer Lentivirus containing hTERT (109 IU/ml)


  2. Sku: LV674
    Price: $750.00
    Unit: 10 ml
    Lentivirus containing hTERT (106 IU/ml)


  3. Sku: LV665
    Price: $1,350.00
    Unit: 5 x 20 μl
    High Titer Lentivirus expressing SV40 large T antigen (109 IU/ml)


  4. Sku: LV664
    Price: $675.00
    Unit: 10 ml
    Lentivirus expressing SV40 large T antigen (106 IU/ml)


  5. Sku: LV667
    Price: $1,350.00
    Unit: 5 x 20 μl
    High Titer Lentivirus containing HPV-16 E6/E7 gene (109 IU/ml)


  6. Sku: LV666
    Price: $675.00
    Unit: 10 ml
    Lentivirus containing HPV-16 E6/E7 gene (106 IU/ml)


  7. Sku: LV660
    Price: $1,350.00
    High Titer Lentivirus expressing SV40 large T antigen (109 IU/ml)


  8. Sku: LV659
    Price: $675.00
    Unit: 10 ml
    Lentivirus expressing SV40 large T antigen (106 IU/ml)


  9. Sku: LV677
    Price: $1,750.00
    Unit: 5 x 20ul
    High Titer Recombinant CDK4 Lentivirus containing hTERT (109 IU/ml)


  10. Sku: LV676
    Price: $995.00
    Unit: 10ml
    Recombinant CDK4 Lentivirus containing hTERT (106 IU/ml)

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