Cell Immortalization Kits
Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.
Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience,abmhas developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.
| Gene Overexpressed | Viruses Offered | Application |
|---|---|---|
| hTERT | Adenovirus, Lentivirus, Retrovirus |
Reliable and commonly used. |
| SV40 T Antigen | Adenovirus, Lentivirus, Retrovirus |
Reliable and commonly used. Often used to immortalize epithelial cells. |
| EBV genes | Epstein-Barr Virus | Immortalize B lymphocytes, and sometimes T lymphocytes. |
| HPV E6-E7 | Lentivirus | Immortalize keratinocytes. |
| HOXB8, HOXA9, HOXA10 | Lentivirus | Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells. |
| Adenoviral E1A-E1B | Lentivirus | Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells. |
| p53 siRNA, Rb siRNA | Lentivirus | Co-express with hTERT for better immortalization of epithelial cells. |
| Bmi1 | Lentivirus | Immortalize epithelial cells. |
| CDK4 | Lentivirus | Co-express with hTERT for a more authentic cell model. |
| Myc T58A (c-Myc), Ras V12 (H-Ras) | Lentivirus | Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.). |
Popular Products
- ViralPlus Transduction Enhancer
- GFP Control Lentivirus (Neo)
- PriCoat Flasks
- Lenti-hTERT (No Selection Marker) virus
- Immortalized Cells
Tools & Resources
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Cell Immortalization Handbook
This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents. -
Cell Immortalization Workflow
Overview of abm's Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base. -
Custom Cell Immortalization Service
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Browse Products
- Sku: G205
Price: $950.00
Unit: 1.0mlAdenovirus containing hTERT (106 IU/ml)
- Sku: G208
Price: $850.00
Unit: 1.0mlAdenovirus containing antisense hTERT (106 IU/ml)
- Sku: G210
Price: $950.00
Unit: 250 μlAdenovirus expressing SV40 large and small T antigens (106 pfu/ml)
- Sku: LV651
Price: $750.00
Unit: 10 mlRecombinant Adenoviral E1A-E1B Lentivirus (10 6 IU/ml)
- Sku: LV652
Price: $1,450.00
Unit: 5 x 20 μlHigh titer recombinant Adenoviral E1A-E1B Lentivirus (10 9 IU/ml)
- Sku: LV610
Price: $750.00
Unit: 10mlRecombinant Bmi1 Lentivirus (106 IU/ml)
- Sku: LV608
Price: $1,450.00
Unit: 5 x 20 μlHigh Titer Recombinant Bmi1 Lentivirus (109 IU/ml)
- Sku: LV611
Price: $750.00
Unit: 10mlRecombinant CDK4 Lentivirus (106 IU/ml)
- Sku: LV609
Price: $1,450.00
Unit: 5 x 20 μlHigh Titer Recombinant CDK4 Lentivirus (109 IU/ml)
- Sku: LV625
Price: $995.00
Unit: 10mlLentivirus containing hTERT with a RFP Reporter & resistance to Puromycin (106 IU/ml)