Lenti-HPV E6/E7 (Puro) Virus, High Titer
| Cat. No. | LV617 |
| Name | Lenti-HPV E6/E7 (Puro) Virus, High Titer |
| Unit | 2 x 100 µl |
| Unpacking and Storage Instructions |
Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Description |
High Titer (109 IU/ml) Recombinant Lentivirus expressing the HPV-16 E6/E7 gene. |
| Application |
Cell immortalization of keratinocytes. |
| Expression System Type | Lentivirus |
| Caution |
This product is distributed for laboratory research only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV617 |
Print/Download Datasheet
| What is the general procedure for immortalizing cells? | |
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See our Cell Immortalization Handbook for more details.
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| Can you recommend a specific reagent and protocol to immortalize my cell line? | |
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Successful cell immortalization is unpredictable and must be determined experimentally, as results vary with species, tissue origin, growth characteristics and whether or not specific cell functions need to be preserved.
Our in-house experiments using the SV40T and hTERT lentiviruses have achieved the highest immortalization success rates in a variety of different cell types. |
| Do immortalized cells retain all characteristics of the original primary cell line? | |
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Not always. Some phenotypic or genetic changes may occur during immortalization. We recommend using our hTERT immortalization reagents as this method preserves phenotype and karyotype as well as minimizes genomic instability.
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| Do I need a control when performing cell immortalization experiments? | |
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Yes. Always include control cells that are not transduced in order to compare growth rates and detect senescence bypass.
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| How do I confirm successful cell immortalization? | |
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Assess long-term growth beyond normal passage limits and verify expression of the immortalizing genes via PCR, qPCR or Western blot.
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| Where can I learn more about cell immortalization? | |
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Check out our Learning Resources and Youtube video for more information.
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| How do the E6/E7 genes immortalize cells? | |
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The oncogenes E6 and E7 from HPV work by targeting the cell's tumor suppressor pathways (p53 and Rb) effectively over riding senescence and apoptosis checkpoints.
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| Will this virus immortalize my primary cells? | |
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This is highly dependent on the immortalization reagent and cell type. Check out our Cell Immortalization Reagents Compatibility Chart for more information.
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- He, C., Lv, X., Liu, J., Ruan, J., Chen, P., Huang, C., Angeletti, P. C., Hua, G., Moness, M. L., Shi, D., Dhar, A., Yang, S., Murphy, S., Montoute, I., Chen, X., Islam, K. N., George, S., Ince, T. A., Drapkin, R., … Wang, C. (2024). HPV-YAP1 oncogenic alliance drives malignant transformation of fallopian tube epithelial cells. EMBO Reports, 25(10), 4542–4569. https://doi.org/10.1038/s44319-024-00233-3
- Huang, C., Lv, X., Chen, P., Liu, J., He, C., Chen, L., Wang, H., Moness, M. L., Dong, J., Rueda, B. R., Davis, J. S., & Wang, C. (2022). Human papillomavirus targets the YAP1-LATS2 feedback loop to drive cervical cancer development. Oncogene, 41(30), 3761–3777. https://doi.org/10.1038/s41388-022-02390-y
- Jenkins, L., Jungwirth, U., Avgustinova, A., Iravani, M., Mills, A., Haider, S., Harper, J., & Isacke, C. M. (2022). Cancer-Associated Fibroblasts Suppress CD8+ T-cell Infiltration and Confer Resistance to Immune-Checkpoint Blockade. Cancer Research, 82(16), 2904–2917. https://doi.org/10.1158/0008-5472.can-21-4141
- Choi, K. Y., El-Hamdi, N., & McGregor, A. (2022). Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein. Journal of Virology, 96(17), e00831-22. https://doi.org/10.1128/jvi.00831-22
- Lee, Y., Glorián Berríos-Vázquez, Maes, R. K., Matti Kiupel, Lowiese M.B. Desmarets, Nauwynck, H. J., & Gisela Soboll Hussey. (2023). Development of immortalized feline respiratory epithelial cells in an air-liquid-interface culture system for feline herpesvirus-1 study. Virus Research, 326, 199063–199063. https://doi.org/10.1016/j.virusres.2023.199063
- Shishi He, Xiaoyun Xiao and Rong Lei et al. Establishment of Breast Phyllodes Tumor Cell Lines Preserving the Features of Phyllodes Tumors. BIOI. 2023. Vol. 4(1):7-17. DOI: 10.15212/bioi-2022-0025. Application: Cell Immortalization.
- Riachi, M et al. Cholesterol Pathway Gene Variants and Reduced Keratinocyte Cholesterol Support a Final Common Druggable Pathway in Hyperproliferative Inflammatory Skin Diseases. Journal of Investigative Dermatology, Volume 145, Issue 11, 2025, Pages 2745-2752.e7, https://doi.org/10.1016/j.jid.2025.02.157. Application: Cell Immortalization
- Saliha Beyza Sayar, Cristina Has. Strategy for the Optimization of Read-Through Therapy for Junctional Epidermolysis Bullosa with COL17A1 Nonsense Mutation. Journal of Investigative Dermatology, Volume 144, Issue 10, 2024, Pages 2221-2229.e1, https://doi.org/10.1016/j.jid.2024.02.027. Application: Cell Immortalization
- Alireza Baradaran-Heravi, Aruna D Balgi, Sara Hosseini-Farahabadi, Kunho Choi, Cristina Has, Michel Roberge, Effect of small molecule eRF3 degraders on premature termination codon readthrough, Nucleic Acids Research, Volume 49, Issue 7, 19 April 2021, Pages 3692–3708, https://doi.org/10.1093/nar/gkab194. Application: Cell Immortalization
- He C, Lv X, Liu J, Ruan J, Chen P, Huang C, Angeletti PC, Hua G, Moness ML, Shi D, Dhar A, Yang S, Murphy S, Montoute I, Chen X, Islam KN, George S, Ince TA, Drapkin R, Guda C, Davis JS, Wang C. HPV-YAP1 oncogenic alliance drives malignant transformation of fallopian tube epithelial cells. EMBO Rep. 2024 Oct;25(10):4542-4569. doi: 10.1038/s44319-024-00233-3. PMID: 39271776. Application: Cell Immortalization
- Liu X, Xia F, Wu X, Tang Y, Wang L, Sun Q, Xue M, Chang W, Liu L, Guo F, Yang Y and Qiu H (2021) Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles. Front. Immunol. 12:759176. doi: 10.3389/fimmu.2021.759176. Application: Cell Immortalization
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