ViralEntry™ Transduction Enhancer (100X)
| Cat. No. | G515 | ||||||||||||||||||||||||||||||||||
| Name | ViralEntry™ Transduction Enhancer (100X) | ||||||||||||||||||||||||||||||||||
| Unit | 1.0 ml | ||||||||||||||||||||||||||||||||||
| Description |
Boost transduction by over 10X in a variety of cell types! As an industry leader in recombinant viral vectors, abm has 15+ years of experience in how to enhance virus vector transduction efficiency. Over the years, we have developed multiple formulations of viral enhancing agents useful for this purpose. A remarkable breakthrough by our top scientists in 2020 is the development of the most efficient viral enhancing agent, ViralEntry™, that is not only effective in enhancing lentivirus transduction efficiency significantly, but also increases transduction efficiency in diverse cell types including primary T lymphocytes by over 10X. This is superior to any similar product on the market. With this incredible reagent, you will be able to transduce any cell types you have. We guarantee that this is the most effective viral transduction reagent in the industry and will gladly refund you if you find better transduction enhancers than our ViralEntry™ formulation. Use it and you will enjoy it! Product Features
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| Storage Condition |
Store at 4°C. In rare instances, precipitates may form but do not affect reagent function and can be dissolved by warming at 37°C for 3-5 minutes before use. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G515 |
| Can I store ViralEntry Transduction Enhancer (100X) at -80°C? Will it reduce the efficiency? | |
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Storage at -80°C will result in a slight reduction in infection efficiency. You can still use it if you have already stored it at -80°C, since the infection efficiency will still be better when compared to the use of no transduction enhancer or the use of polybrene alone. However for optimal results, it is best to store #G515 unfrozen at 4°C.
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| Are ViralEntry™ and AAViralEntry™ equivalent to ViralMax and ViralPlus? | |
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ViralEntry™ and AAViralEntry™ are abm’s next generation of Viral Transduction Reagents and are functionally equivalent to Cat. No. G698, G511, G512, G513, and G514 (ViralMax, and ViralPlus Transduction Reagents), with improved performance. Contact our customer service team at technical@abmgood.com for more information.
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| Does polybrene still need to be added if ViralEntry Tranduction Enhancer is used? | |
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Our ViralEntry™ Transduction Enhancer (100X) (Cat. G515) is designed to be used without the need of polybrene. Simply add the enhancer at 1:100 to culture medium, mix gently, and infect each well with your virus. |
- Joladarashi, D., Thej, C., Mallaredy, V., Magadum, A., Cimini, M., Gonzalez, C., Truongcao, M., Nigro, J. T., Sethi, M. K., Gibb, A. A., Benedict, C., Koch, W. J., & Kishore, R. (2024). GPC3-mediated metabolic rewiring of diabetic mesenchymal stromal cells enhances their cardioprotective functions via PKM2 activation. IScience, 27(10), 111021. https://doi.org/10.1016/j.isci.2024.111021
- Bento, R., Scheller, J., & Parekkadan, B. (2024). Intratumoral Delivery of Genetically Engineered Anti-IL-6 Trans-signaling Therapeutics. Molecular Biotechnology. https://doi.org/10.1007/s12033-024-01230-6
- Nayak, T. K., Bajpai, A., Patwa, V., Carter, R. L., Enjamuri, N., Gao, E., Xiang, Y. K., & Tilley, D. G. (2023). Loss of Myeloid Cell-Specific β2-Adrenergic Receptor Expression Ameliorates Cardiac Function and Remodeling after Acute Ischemia. https://doi.org/10.1101/2023.11.27.568873
- Tang, R., Shuldiner, E. G., Kelly, M., Murray, C. W., Hebert, J. D., Andrejka, L., Tsai, M. K., Hughes, N. W., Parker, M. I., Cai, H., Li, Y.-C., Wahl, G. M., Dunbrack, R. L., Jackson, P. K., Petrov, D. A., & Winslow, M. M. (2023). Multiplexed screens identify RAS paralogues HRAS and NRAS as suppressors of KRAS-driven lung cancer growth. Nature Cell Biology, 25(1), 159–169. https://doi.org/10.1038/s41556-022-01049-w
-
Liu, X., Xia, F., Wu, X., Tang, Y., Wang, L., Sun, Q., ... & Qiu, H. (2021). Isolation of primary mouse pulmonary microvascular endothelial cells and generation of an immortalized cell line to obtain sufficient extracellular vesicles. Frontiers in Immunology, 12, 759176. https://doi.org/10.3389/fimmu.2021.759176
Ortiz, M. M., Patel, D. M., García-Lerena, J. A., Nelson, A. C., Swiatnicki, M., & Andrechek, E. (2024). Unraveling the role of receptor-like protein tyrosine phosphatase PTPRH in cell signaling regulation and biological processes of non-small cell lung cancer. bioRxiv, 2024-06. https://doi.org/10.1101/2024.06.13.598886
Zhu, F., Zhang, R., He, Z., Zhang, N., Li, F., Li, J., ... & Zhong, F. (2026). Indole-3-carbinol attenuates cisplatin-induced premature ovarian failure by activating Nrf2 through competitive binding of Keap1. Phytomedicine, 158040. https://doi.org/10.1016/j.phymed.2026.158040
Thej, C., Roy, R., Cheng, Z., Garikipati, V. N. S., Truongcao, M. M., Joladarashi, D., ... & Kishore, R. (2024). Epigenetic mechanisms regulate sex differences in cardiac reparative functions of bone marrow progenitor cells. npj Regenerative Medicine, 9(1), 17. https://doi.org/10.1038/s41536-024-00362-2
Rodriguez Moncivais, O. J. (2025). Jak1 V658f Drives Oncogenic Transformation Via Transcriptional Reprogramming Independent Of Y598 Phosphorylation And Canonical Jak-Stat Signaling. https://www.proquest.com/openview/c0ea401074d07eab12fe332e5b39acff/1
Suarez-Rivero, J. M., Muela-Zarzuela, I., Boy-Ruiz, D., Varga-Martínez, R., García-Cuesta, D., & Cordero, M. D. (2025). NLRP1 inflammasome drives IL-18 in Familial Mediterranean Fever and MEFV variants. https://doi.org/10.21203/rs.3.rs-7037096/v1
Prinz, F., Jonas, K., Balihodzic, A., Klec, C., Reicher, A., Barth, D. A., ... & Pichler, M. (2022). MicroRNA mimics can distort physiological microRNA effects on immune checkpoints by triggering an antiviral interferon response. RNA biology, 19(1), 1305-1315. https://doi.org/10.1080/15476286.2022.2152978
Gonzalez, C., Cimini, M., Cheng, Z., Benedict, C., Wang, C., Trungcao, M., ... & Kishore, R. (2022). Role of circular RNA cdr1as in modulation of macrophage phenotype. Life sciences, 309, 121003. https://doi.org/10.1016/j.lfs.2022.121003
Bai, X., Guo, Y. R., Zhang, W. C., Shi, Z. H., & Dai, D. Q. (2026). Targeting the ZDHHC9-mediated STAT1 palmitoylation-phosphorylation conversion inhibits gastric cancer progression. Journal of Gastroenterology, 1-17. https://doi.org/10.1007/s00535-026-02363-y
García-Cuesta, D. Juan Miguel Suarez-Rivero 1 Inés Muela-Zarzuela 1 Daniel Boy-Ruiz 1 Raquel de la. https://assets-eu.researchsquare.com/files/rs-7037096/v1/27303880ab677239ff019afe.html
Gonzalez, C. (2024). The Role of Circular RNA Cdr1as in Macrophage Mediated Cardiac Injury and Repair. Temple University. https://doi.org/10.1016/j.lfs.2022.121003
Fernando, S. J., Wang, Q., Hay, D. L., Bathgate, R. A., Shepherd, P. R., & Lee, K. L. (2023). Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Bioscience Reports, 43(4), BSR20221956. https://doi.org/10.1042/bsr20221956
Žbulj, V. (2023). Establishment of a CD8+ T-cell activation protocol via APC and CD3/CD28 activation/Žbulj Vito, BSc. https://unipub.uni-graz.at/obvugrhs/content/titleinfo/8547715/full.pdf
Ichinose, M., Suzuki, N., Wang, T., Wright, J. A., Lannagan, T. R., Vrbanac, L., ... & Woods, S. L. (2021). Stromal DLK1 promotes proliferation and inhibits differentiation of the intestinal epithelium during development. American Journal of Physiology-Gastrointestinal and Liver Physiology, 320(4), G506-G520. https://doi.org/10.1152/ajpgi.00445.2020
Ali, G., Gibbard, D., Ghelfi, E., Olson, A., Cai, J., Balagtas, A., ... & Brownfield, D. G. (2026). Membrane Tension Integrates Physical and Signaling Cues to Gate Cell Fate Transitions. bioRxiv, 2026-03. https://doi.org/10.64898/2026.03.04.708749 - Liu X, Xia F, Wu X, Tang Y, Wang L, Sun Q, Xue M, Chang W, Liu L, Guo F, Yang Y and Qiu H (2021) Isolation of Primary Mouse Pulmonary Microvascular Endothelial Cells and Generation of an Immortalized Cell Line to Obtain Sufficient Extracellular Vesicles. Front. Immunol. 12:759176. doi: 10.3389/fimmu.2021.759176. Application: Cell Immortalization

