IHC Staining

Immunohistochemistry (IHC) is currently used in both research and clinical settings for the diagnosis of abnormal cells and the distribution and localization of certain proteins-of-interest in the cell. abm offers IHC staining services using a wide range of validated antibodies- over 200 high specificity antibodies for a variety of pathological markers.

With fast turn-around times and competitive prices, we can help you detect any marker you desire with high sensitivity and minimum background. All staining will be done automatically using the Leica BOND-MAX Autostainer to guarantee consistent results. Send us your unstained sectioned FFPE tissue slide and we will do the staining for you.

In addition to IHC staining, abm’s full range of histology services includes tissue or cell pellet processing, embedding, sectioning and routine or special staining for paraffin samples.

Workflow




"We are happy about the CD4 and CD8 IHC staining your lab did. The quality of the IHC staining is very good."

Fugang Zhu, Idera Pharnacueticals Inc, Custom IHC Staining Service (HC002)

Additional Resources:

Service Details

Core Services
Service NameDescriptionCAT. NO.PRICE
Immunohistochemistry Staining-Basic Service
(Primary antibody provided by customer)
Immunohistochemistry staining of previously sectioned FFPE slides†.

Customers to provide:
(1) a minimum of 20 μg of the primary antibody raised in either Mouse or Rabbit
(2) information regarding the antigen retrieval method and optimal antibody working concentration for the marker of interest, and
(3) one positive control slide for the marker of interest.
(4) tissue blocks or slides for the marker of interest.

Deliverables: Cover-slipped stained IHC slide.

Please consult our IHC Antibody Optimization page for newly developed antibodies with unknown conditions.
HC001 $25.00/slide
Immunohistochemistry Staining- Complete Service
(Primary antibody provided by abm)
Immunohistochemistry staining of previously sectioned FFPE slides† with one of abm’s repertoire of common antibodies. Please contact us for our list of validated IHC antibodies for this service.

Customers to provide:
(1) tissue blocks or slides for the marker of interest.

Deliverables: Cover-slipped stained IHC slide.
HC002 $35.00/slide
Immunohistochemistry Staining-Dual Staining Service Immunohistochemistry staining of previously sectioned FFPE slides†. Dual staining service offers simultaneous staining of two markers-of interest. This service is restricted to the simultaneous detection of one (1) Mouse antibody (Stained brown) and one (1) Rabbit Antibodies (Stained red).

Customers to provide:
(1) a minimum of 20 μg of each primary antibody
(2) information regarding optimal antibody dilution for both antibodies
(3) one positive control slide for each of the marker of interest.
(4) tissue blocks or slides for the marker of interest.

Deliverables: Cover-slipped stained IHC slide.

HC003 Inquire - starting at $55.00/slide
†Please section your tissue at 4μm and mount them onto charged slides. Turnaround time is 1-2 weeks. For rush orders, please inquire.
Add-On Services*
Have the tissue, but can’t embed it or cut it? Don’t worry, send us the tissue sample/block, and we can do the embedding and/or cutting for you!<!----!>
Add‑On Services*CAT. NO.PRICE
HC004 $5.00/tissue
Processing of Formalin-Fixed wet tissue suitable for paraffin embedding.

Deliverables: Processed wet tissue suitable for paraffin embedding.
HC005 $4.00/tissue
Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining.

Deliverables: FFPE tissue block
HC009 $8.00/block
Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining.

Deliverables: Paraffin embedded cell pellet block
HC006 $4.00/Slide
Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining. All slides are cut at 4 μM unless otherwise specified. Please inquire for Tissue Microarray sectioning.

Deliverables: Unstained FFPE tissue section slides.
HC007 Inquire - starting at $6.00/slide
Multiple sections from the same tissue block cut at set distances from one another by the Microtome

Deliverables: Multiple slides cut in sequence by the Microtome from the same specimen.
HC008 Inquire - starting at $6.00/slide
Multiple sections from the same tissue block cut at set distances from one another by the Microtome

Deliverables: Multiple slides within a sample at set distance from one another
HC016 Inquire
HC017 Inquire
HC018 Inquire
Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services.

Deliverables: Digital scan will be digitally provided to the customer.
HC011 Inquire
Image analysis of stained slides using professional software.

Deliverables: Analysis results will be sent digitally to the client.
Notes:
*Please note all Add-On Services pricing is subject to ordering with HC001, HC002 or HC003. If ordered as a stand-alone service, pricing is subject to change, please inquire for more details.
#Minimum order of 10 tissue samples is required.

Additional Info

Sample Submission Guidelines and Shipping Instructions
Please fill out our Histology Service Request Form, and email it to [email protected] to initiate an inquiry. Before shipping, please fill out our Histology Services Sample Submission Formwith your order number and follow the shipping instructions for your samples.
Specimen typeShipping instructions
Unstained slides Place slides in microscope slide boxes. Make sure to seal and wrap the box well.
Formalin-Fixed wet tissues Transfer formalin-fixed sample to 70% alcohol. Seal and wrap the container well to prevent leaks.
Fixed cell pellet Transfer the samples to 70% alcohol. Seal and wrap the container well to prevent leaks.
FFPE tissue blocks Ship tissue blocks in a cassette case or bag. During the month of May to August, ship blocks with gel ice pack to prevent the block from melting due to higher temperatures.

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