Custom CRISPR Knock-in Repair Templates
CRISPR Knock-in allows for the precise desired mutation to be made anywhere in the genome! abm offers Custom CRISPR Knock-in Repair Templates where we can design and clone your HDR Template and sgRNA/Cas9 expression vectors (Custom CRISPR sgRNA Vectors and Viruses). For small mutations (<50bp) we recommend using our ssDNA Oligo Repair Templates which are designed with two flanking phosphorothioate bonds, while larger mutations should be handled with one of our dsDNA vectors. Not sure where to begin? Simply click the Inquire Button and tell us about your custom project.
Applications
- In-frame fusion tags - Tag your gene of interest with a short peptide sequence
- Point mutations - Mutate short sequences or residues within your gene of interest
- Transgene insertion - Insert a larger transgene or reporter into a locus of your choice
Additional Resources:
Service Details
ssDNA Oligonucleotide Repair Template
| Service Name | Homology Arms + Repair Template | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Repair Template (<200nt ssDNA oligonucleotide) | Homology arms + insert must be less than 200nt total. Suggested homology arm size is 50nt-90nt per arm | 4 nmole | C427 | $275.00 |
- Lyophilized oligonucleotide with 5' and 3' phosphorothioate bonds. Contains your insert with flanking homology arms.
dsDNA Vector Repair Template (homology arms <1.2 kb)
| Service Name | Repair Insert | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Repair Template (< 1.2 kb arms, < 1 kbp insert) | Custom insert < 1 kbp | 1.0 ug | C428 | $615.00 |
| Custom CRISPR Repair Template (< 1.2 kb arms, 1-2 kbp insert) | Custom insert 1-2 kbp | 1.0 ug | C429 | $795.00 |
| Custom CRISPR Repair Template (< 1.2 kb arms, SV40-GFP-2A-Puro insert) | Predesigned insert with GFP and Puromycin resistance | 1.0 ug | C430 | $875.00 |
| Custom CRISPR Repair Template (< 1.2 kbp arms, SV40-RFP-2A-Puro insert) | Predesigned insert with RFP and Puromycin resistance | 1.0 ug | C431 | $875.00 |
- Plasmid with Homology Arms up to <1.2kb (both left and right arms)
- dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is < 1.2kb in length)
dsDNA Vector Repair Template (homology arms 1.2-2 kb)
| Service Name | Repair Insert | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Repair Template (1.2-2 kb arms, < 1 kbp insert) | Custom insert < 1 kbp | 1.0 ug | C432 | $855.00 |
| Custom CRISPR Repair Template (1.2-2 kb arms, 1-2 kbp insert) | Custom insert 1-2 kbp | 1.0 ug | C433 | $1025.00 |
| Custom CRISPR Repair Template (1.2-2 kb arms, SV40-GFP-2A-Puro insert) | Predesigned with GFP and Puromycin resistance | 1.0 ug | C434 | $1095.00 |
| Custom CRISPR Repair Template (1.2-2 kb arms, SV40-RFP-2A-Puro insert) | Predesigned with GFP and Puromycin resistance | 1.0 ug | C435 | $1095.00 |
- dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is 1.2-2kb in length)
dsDNA Vector Repair Template (homology arms >2.0 kb)
| Service Name | Repair Insert | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Repair Template (>2 kbp arms, any insert size) | Custom or predesigned insert | 1.0 ug | C436 | Inquire |
- dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is >2kb in length)
Additional Info
Available Custom CRISPR Vectors and Viruses:
| AAV Vector /Virus |
Lentivector /Virus |
Non-Viral Vector |
Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| Browse Collection | Browse Collection | Browse Collection | Browse Collection |
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Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359 |