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Custom CRISPR Knock-in Repair Templates

CRISPR Knock-in allows for the precise desired mutation to be made anywhere in the genome! abm offers Custom CRISPR Knock-in Repair Templates where we can design and clone your HDR Template and sgRNA/Cas9 expression vectors (Custom CRISPR sgRNA Vectors and Viruses). For small mutations (<50bp) we recommend using our ssDNA Oligo Repair Templates which are designed with two flanking phosphorothioate bonds, while larger mutations should be handled with one of our dsDNA vectors. Not sure where to begin? Simply click the Inquire Button and tell us about your custom project. 

Applications

  • In-frame fusion tags - Tag your gene of interest with a short peptide sequence
  • Point mutations - Mutate short sequences or residues within your gene of interest
  • Transgene insertion - Insert a larger transgene or reporter into a locus of your choice





Service Details

ssDNA Oligonucleotide Repair Template
Service Name Homology Arms + Repair Template Quantity Cat. No. Price
Custom CRISPR Repair Template (<200nt ssDNA oligonucleotide) Homology arms + insert must be less than 200nt total. Suggested homology arm size is 50nt-90nt per arm 4 nmole C427 $275.00
  • Lyophilized oligonucleotide with 5' and 3' phosphorothioate bonds. Contains your insert with flanking homology arms.

dsDNA Vector Repair Template (homology arms <1.2 kb)
Service Name Repair Insert Quantity Cat. No. Price
Custom CRISPR Repair Template (< 1.2 kb arms, < 1 kbp insert) Custom insert < 1 kbp 1.0 ug C428 $615.00
Custom CRISPR Repair Template (< 1.2 kb arms, 1-2 kbp insert) Custom insert 1-2 kbp 1.0 ug C429 $795.00
Custom CRISPR Repair Template (< 1.2 kb arms, SV40-GFP-2A-Puro insert) Predesigned insert with GFP and Puromycin resistance 1.0 ug C430 $875.00
Custom CRISPR Repair Template (< 1.2 kbp arms, SV40-RFP-2A-Puro insert) Predesigned insert with RFP and Puromycin resistance 1.0 ug C431 $875.00
  • Plasmid with Homology Arms up to <1.2kb (both left and right arms)
  • dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is < 1.2kb in length)

dsDNA Vector Repair Template (homology arms 1.2-2 kb)
Service Name Repair Insert Quantity Cat. No. Price
Custom CRISPR Repair Template (1.2-2 kb arms, < 1 kbp insert) Custom insert < 1 kbp 1.0 ug C432 $855.00
Custom CRISPR Repair Template (1.2-2 kb arms, 1-2 kbp insert) Custom insert 1-2 kbp 1.0 ug C433 $1025.00
Custom CRISPR Repair Template (1.2-2 kb arms, SV40-GFP-2A-Puro insert) Predesigned with GFP and Puromycin resistance 1.0 ug C434 $1095.00
Custom CRISPR Repair Template (1.2-2 kb arms, SV40-RFP-2A-Puro insert) Predesigned with GFP and Puromycin resistance 1.0 ug C435 $1095.00
  • dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is 1.2-2kb in length)

dsDNA Vector Repair Template (homology arms >2.0 kb)
Service Name Repair Insert Quantity Cat. No. Price
Custom CRISPR Repair Template (>2 kbp arms, any insert size) Custom or predesigned insert 1.0 ug C436 Inquire
  • dsDNA vector containing an insert with flanking homology arms (total homology arms sequence is >2kb in length)

Additional Info

Workflow

Available Custom CRISPR Vectors and Viruses:
AAV Vector
/Virus
Lentivector
/Virus
Non-Viral
Vector
Adenovirus
sgRNA for spCas9 Available Available Available Available
sgRNA for saCas9 Available - - -
All-in-One with spCas9 - Available Available -
All-in-One with saCas9 Available - - -
All-in-One with FnCas12a (FnCpf1) - Available - -
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FAQs

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Citations

01 Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371
02 Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338
03 Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359
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