Custom CRISPR Knock-in Repair Templates
CRISPR Knock-in allows for the precise desired mutation to be made anywhere in the genome! abm offers Custom CRISPR Knock-in Repair Templates where we design, synthesize and clone your Homology Directed Repair (HDR) Template and corresponding sgRNA/Cas9 expression vectors. For small mutations (<50bp) we recommend using our ssDNA Oligonucleotide Repair Templates, while larger constructs should be handled with our dsDNA vectors. Not sure where to begin? Inquire with us below and get started on your custom CRISPR Knock-in project.
Applications:
- In-frame fusion tags - Tag your gene of interest with a short peptide sequence
- Point mutations - Mutate short sequences or residues within your gene of interest
- Transgene insertion - Insert a larger transgene or reporter into a locus of your choice
Service Details
ssDNA Oligonucleotide Repair Template
| Service Name | Description | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Repair Template (<200nt ssDNA oligonucleotide) | Lyophilized oligonucleotide with 5' and 3' phosphorothioate bonds. Contains your insert with flanking homology arms. | 4 nmol | C427 |
dsDNA Vector Repair Template
| Service Name | Description | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR Non-Viral Vector Repair Template | dsDNA Non-Viral Vector containing your custom insert with 500 bp each flanking homology arms | 1.0 µg | C428 |
- Custom Insert must not exceed 2.0 kb in length. For larger insert sizes, please inquire.
Cas9 & sgRNA Expression Vectors
| Service Name | Vector | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR All-in-One Lentiviral Vector (with spCas9) - set of three targets | pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro | 3 x 1.0 µg | C442 | |
| Custom CRISPR Cas9 All-in-One AAV Vector (with saCas9) - set of three targets | pAAV-PGK-saCas9-U6-sgRNAsa | 3 x 1.0 µg | C306 | |
| Custom CRISPR All-in-One Non-Viral Vector (with spCas9) - set of three targets | pNV-sgRNA-Cas9-2A-GFP | 3 x 1.0 µg | C444 |
- Viral packaging is also available upon request.
Additional Info
Available Custom CRISPR Vectors & Viruses
| AAV Vector/Virus | Lentivector/Virus | Non-Viral Vector | Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| All-in-One with AaCas12b | - | Available | - | - |
| sgRNA for AaCas12b | Available | Available | - | - |
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Additional Resources
Documents
Citations
| 01 | Fleck JS, et al. "Inferring and perturbing cell fate regulomes in human brain organoids." Nature (2022). doi: 10.1038/s41586-022-05279-8 |
| 02 | Das A, et al. "Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus." Nature Microbiology (2020). doi: 10.1038/s41564-020-0727-8 |
| 03 | Yamane D, et al. "Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses." Nature Microbiology (2019). doi: 10.1038/s41564-019-0425-6 |