CRISPR Activation & Repression

CRISPR Activation (CRISPRa) allows for gene specific up-regulation by using dCas9-VPR. This fusion protein consists of the catalytically dead Cas9 (dCas9) enzyme fused to the tripartite complex VPR (VP64, p65 and Rta). sgRNAs are designed to target and guide dCas9-VPR to the upstream 5' UTR promoter region of a specific gene thereby resulting in transcriptional up-regulation. 

Key Features

  • Comprehensive collection of gene activation sgRNAs
  • Targets include all human, mouse and rat genes.
  • Available in a set of 3 sgRNA vectors/viruses for added assurance of efficient gene activation
  • Lentiviral delivery results in host integration of DNA, leading to long term gene expression
  • Lentiviral vectors can be transfected for non-integrating, transient sgRNA expression

Looking for constructs expressing multiple sgRNAs? Check out our Custom Multiplex sgRNA Service.  

For Custom Activation/Repression sgRNAs, order here.  

Search Activation sgRNA Library

Activation sgRNA Lentivirus Library

We offer sgRNA lentivectors & lentiviruses for gene activation of any human, mouse, or rat gene.

Product Information

  • pLenti-U6-sgRNA-PGK-Neo vector map
    Vector Map
    sgRNA for activation of your gene of interest is available in lentiviral vector or lentivirus format.
  • CRISPR dCas9 VPR Lentivector Lentivirus Workflow
    How to use abm's activation sgRNA lentiviruses in your CRISPR activation experiment.
  • CRISPR Activation and Repression Knowledge Base
    Need Help Getting Started?
    Read our CRISPR Activation and Repression Knowledge Base article for more information.

Top Publications

Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.

Kang, Y J et al.
Nat. Commun. 6:8371 (2015).

DOI: 10.1038/ncomms9371.

Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting through the proto-oncogene MET.

Shirasaki, T. et al.
Nature Communications. (2022).

doi: 10.1038/s41467-022-30879-3

Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer.

Okugawa, Y et al.
Gut (2015)


Regulation and tumor-suppressive function of the miR-379/miR-656 (C14MC) cluster in cervical cancer.

Srinath, S et al.
Mol Oncol. (2024)

doi: 10.1002/1878-0261.13611