CRISPR Activation
abm's CRISPR Activation sgRNA vectors and viruses are highly effective at achieving activation of your target gene. CRISPR Activation (CRISPRa) allows for gene specific up-regulation by using dCas9-VPR. This fusion protein consists of the catalytically dead Cas9 (dCas9) enzyme fused to the tripartite complex VPR (VP64, p65 and Rta). We offer a comprehensive collection of sgRNA-only lentiviral vectors expressing sgRNAs that target the upstream 5' UTR promoter region of any human, mouse or rat gene thereby resulting in transcriptional up-regulation. Can't find the construct you're looking for? Contact our team for Custom sgRNA and Custom multiple sgRNA services.
Search Activation sgRNA Library
Search our collection of CRISPR gene activation sgRNA products using gene name or accession number:
Additional Information
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Vector Map
sgRNA for activation of your gene of interest is available in lentiviral vector or lentivirus format. -
Workflow
How to use abm's activation sgRNA lentiviruses in your CRISPR activation experiment. -
Need Help Getting Started?
Read our CRISPR Activation and Repression Knowledge Base article for more information.
Documents
Top Publications
Inferring and perturbing cell fate regulomes in human brain organoids.
Fleck JS. et al.
Nature (2022)
doi: 10.1038/s41586-022-05279-8
Gangliosides are essential endosomal receptors for quasi-enveloped and naked hepatitis A virus.
Das A. et al.
Nature Microbiology (2020)
doi: 10.1038/s41564-020-0727-8
Basal expression of interferon regulatory factor 1 drives intrinsic hepatocyte resistance to multiple RNA viruses.
Yamane D. et al.
Nature Microbiology (2019)
doi: 10.1038/s41564-019-0425-6
FAQs
| What are CRISPR Activation vectors and how do they work? |
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These constructs are a collection of lentiviral vectors that express sgRNAs targeting the upstream 5′ UTR of a specific gene. When combined with dCas9-VPR, they mediate targeted gene activation by recruiting transcriptional activators. In contrast, pairing the sgRNAs with dCas9-KRAB results in targeted gene repression through KRAB-mediated transcriptional silencing.
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| What type of Cas9 is compatible with the CRISPR Activation vectors? |
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We recommend using dCas9-VPR for gene activation experiments and dCas9-KRAB for gene repression experiments.
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| How does CRISPR activation differ from traditional CRISPR-Cas9 editing? |
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Unlike CRISPR-Cas9, which introduces permanent DNA double-strand breaks, CRISPRa modulates gene expression by recruiting transcriptional activators to promoter or enhancer regions, leaving the genome unchanged.
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| How long does gene activation last when using CRISPR activation vectors? |
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Gene activation is generally transient when CRISPRa components are delivered episomally, but longer-term activation can be achieved using viral delivery systems or stable cell line generation.
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