CRISPR Activation & Repression
CRISPR Activation (CRISPRa) allows for gene specific up-regulation by using dCas9-VPR. This fusion protein consists of the catalytically dead Cas9 (dCas9) enzyme fused to the tripartite complex VPR (VP64, p65 and Rta). sgRNAs are designed to target and guide dCas9-VPR to the upstream 5' UTR promoter region of a specific gene thereby resulting in transcriptional up-regulation.
Key Features
- Comprehensive collection of gene activation sgRNAs
- Targets include all human, mouse and rat genes.
- Available in a set of 3 sgRNA vectors/viruses for added assurance of efficient gene activation
- Lentiviral delivery results in host integration of DNA, leading to long term gene expression
- Lentiviral vectors can be transfected for non-integrating, transient sgRNA expression
Looking for constructs expressing multiple sgRNAs? Check out our Custom Multiplex sgRNA Service.
For Custom Activation/Repression sgRNAs, order here.
Search Activation sgRNA Library
Activation sgRNA Lentivirus Library
We offer sgRNA lentivectors & lentiviruses for gene activation of any human, mouse, or rat gene.
Can’t find what you’re looking for? Request a custom CRISPR sgRNA virus or vector with [email protected].
Product Information
-
Vector Map
sgRNA for activation of your gene of interest is available in lentiviral vector or lentivirus format. -
Workflow
How to use abm's activation sgRNA lentiviruses in your CRISPR activation experiment. -
Need Help Getting Started?
Read our CRISPR Activation and Repression Knowledge Base article for more information.
Documents
Top Publications
Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.
Kang, Y J et al.
Nat. Commun. 6:8371 (2015).
DOI: 10.1038/ncomms9371.
Leukocyte cell-derived chemotaxin 2 is an antiviral regulator acting through the proto-oncogene MET.
Shirasaki, T. et al.
Nature Communications. (2022).
doi: 10.1038/s41467-022-30879-3
Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer.
Okugawa, Y et al.
Gut (2015)
doi:10.1136/gutjnl-2015-309359
Regulation and tumor-suppressive function of the miR-379/miR-656 (C14MC) cluster in cervical cancer.
Srinath, S et al.
Mol Oncol. (2024)