Reverse Transcription and RT-PCR
abm’s OneScript® and OneScript® Plus Reverse Transcriptases are engineered without intrinsic RNase H activity to increase cDNA synthesis yield and achievable length. OneScript® and OneScript® Plus’s exceptional sensitivity ensure high yields of full length cDNA even with only 0.1 pg of RNA template.
OneScript® can elongate up to 9 kb while OneScript® Plus’s extreme processivity enables it to elongate up to 15 kb. OneScript® Plus performs under high temperatures (45°C - 55°C), making it the better choice for cDNA synthesis from complex RNA templates (i.e. secondary structures and high GC content).
OneScript® and OneScript® Plus are offered as independent enzymes in optimized buffers, cDNA Synthesis Kits, cDNA Synthesis SuperMixes, and a variety of other formats to suit many applications. The table below outlines the formats and their respective components:
|Enzyme Only||Suitable for any application||
|cDNA Synthesis Kit||Flexible priming of oligo(dT) and random primers||
|cDNA Synthesis SuperMix||Minimal pipetting steps||
|All-In-One MasterMix||Ultimate convenience and reproducibility||
|One-Step RT-PCR or RT-qPCR Kit||One kit for downstream application||
MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration
Antony, A., et al.
Nature Communications (2016) 9(7):10955-65.
Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome
Wood, L. W., et al.
Scientific Reports (2016) 6(19857): 1-14.
Request a Free Sample
See for yourself why your fellow PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a "first come first served" basis and shipping charges may apply.
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