Reverse Transcription and RT-PCR

abm’s OneScript® and OneScript® Plus Reverse Transcriptases are engineered without intrinsic RNase H activity to increase cDNA synthesis yield and achievable length. OneScript® and OneScript® Plus’s exceptional sensitivity ensure high yields of full length cDNA even with only 0.1 pg of RNA template.

OneScript® can elongate up to 9 kb while OneScript® Plus’s extreme processivity enables it to elongate up to 15 kb. OneScript® Plus performs under high temperatures (45°C - 55°C), making it the better choice for cDNA synthesis from complex RNA templates (i.e. secondary structures and high GC content).

Product Information

OneScript® and OneScript® Plus are offered as independent enzymes in optimized buffers, cDNA Synthesis Kits, cDNA Synthesis SuperMixes, and a variety of other formats to suit many applications. The table below outlines the formats and their respective components:

FormatFeatureComponents
Enzyme Only Suitable for any application
  • RTase and 5X RT Buffer
cDNA Synthesis Kit Flexible priming of oligo(dT) and random primers
  • RTase and 5X RT Buffer
  • Oligo(dT) and random primers
  • dNTPs
  • RNaseOFF Ribonuclease Inhibitor
cDNA Synthesis SuperMix Minimal pipetting steps
  • RTase
  • 2X Reaction Mix
All-In-One MasterMix Ultimate convenience and reproducibility
  • 5X All-In-One MasterMix
One-Step RT-PCR or RT-qPCR Kit One kit for downstream application
  • RTase
  • RT-PCR or RT-qPCR Enzyme Mix
  • PCR or qPCR MasterMix

Resources

  • Reverse Transcriptase Brochure
    RT-PCR Product Brochure and Performance Data
  • Polymerase Chain Reaction (PCR) -
Reverse Transcription Knowledge Base
    Reverse Transcription (RT-PCR) Knowledge Base
  • PCR RT-PCR qPCR Application Handbook
    PCR RT-PCR qPCR Application Handbook

Top Publications

MICU1 regulation of mitochondrial Ca(2+) uptake dictates survival and tissue regeneration

Antony, A., et al.
Nature Communications (2016) 9(7):10955-65.


Doi: 10.1038/ncomms10955

Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome

Wood, L. W., et al.
Scientific Reports (2016) 6(19857): 1-14.


Doi: 10.1038/srep19857

Replication of a pathogenic non-coding RNA increases DNA methylation in plants associated with a bromodomain-containing viroid binding protein

Ly, D., et al.
Scientific Reports (2016) 6(35651): 1-9.


Doi: 10.1038/srep35751

Request a Free Sample

See for yourself why your fellow PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a "first come first served" basis and shipping charges may apply.

Browse Products

Showing 1-10 of 58

Page
per page
Set Descending Direction

Showing 1-10 of 58

Page
per page
Set Descending Direction