OneScript® Plus cDNA Synthesis Kit

CAT.NOUNITPRICE
G236100 rxn
$150.00

Customize OneScript® Plus cDNA Synthesis Kit
1 x OneScript® Plus Reverse Transcriptase   + $0.00
1 x 5X RT Buffer   + $0.00
1 x Oligo(dT) (10 μM)   + $0.00
1 x Random Primers (10 μM)   + $0.00
1 x dNTP (10 mM)   + $0.00
2 x Nuclease-Free H2O   + $0.00

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OneScript® Plus cDNA Synthesis Kit
OneScript® Plus cDNA Synthesis Kit

In stock

$150.00

Summary
    Specifications


    Description

    (Equivalent to Cat. No. G233, G234, G235, and G491.)
    OneScript® Plus cDNA Synthesis Kit contains a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.


    Key Features

    • Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers
    • Robust cDNA synthesis from any RNA template
    • High reproducibility and excellent yield 
    • Employs abm's OneScript® Plus Reverse Transcriptase (Cat. No. G237)
    • Earn 2X the rewards points

    Request Free Sample

     

    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

     


    Product ComponentQuantity
    OneScript® Plus Reverse Transcriptase 100 rxn (100 µl)
    5X RT Buffer 400 µl
    Oligo(dT) (10 μM) 100 µl
    Random Primers (10 μM) 100 µl
    dNTP (10 mM) 100 µl
    Nuclease-Free H2O 2 x 1.0 ml
    2X the points for PCR Enzymes, Buffers, Solutions, and more!
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    Note: This product is abm’s next generation of cDNA Synthesis Kits and is functionally equivalent to Cat. No. G233, G234, G235, and G491 (OneScript™ and OneScript™ Plus cDNA Synthesis Kits), with improved performance. Contact our customer service team [email protected] for more information.

     
    SKUG236
    Applications
    • Synthesizing cDNA from ssRNA
    • DNA primer extension
    • Sequencing dsDNA
    • Constructing cDNA library
    • Producing template for use in RT-PCR
    • Labelling 3'-end of duplex DNA via end-filling reactions
    • Generating probes for hybridization
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn
    FAQs


    References


    24
    • Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
    • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    • Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell. 13(3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
    • Juskeviciute, E et al. "Inhibition of miR-21 rescues liver regeneration after partial hepatectomy in ethanol-fed rats" Amer. J. Physiol.-Gastrointestinal and Livery Physiology 5:G794-G806 (0). DOI: 10.1152/ajpgi.00292.2016 .
    • Amodio, G et al. "Identification of a microRNA (miR-663a) induced by ER stress and its target gene PLOD3 by a combined microRNome and proteome approach" Cell Bio. Toxicol. 285: (2016). DOI: 10.1007/s10565-016-9335-z.
    • Antony, A et al. "MICU1 regulation of mitochondrial Ca2+ uptake dictates survival and tissue regeneration" Nat. Comm. : (2016). DOI: 10.1038/ncomms10955.
    • Lakshmi , K et al. "A novel comparative pattern analysis approach identifies chronic alcohol mediated dysregulation of transcriptomic dynamics during liver regeneration" BMC Genomics 1:1 (2016). DOI: 10.1186/s12864-016-2492-x.
    • Maher, SK et al. "Rethinking the biological relationships of the thyroid hormones, l-thyroxine and 3,5,3′-triiodothyronine" Comp. Biochem. Physiol. D: Genomics Proteomics :44-53 (2016). DOI: http://dx.doi.org/10.1016/j.cbd.2016.04.002.
    • Diana, D et al. "Inhibition of autocrine signaling of the Epithelial-Mesenchymal Transition in breast cancer" : (2016).
    • Park, H-S et al. "PPARγ neddylation essential for adipogenesis is a potential target for treating obesity" Cell Death Diff. :45-57 (2016). DOI: http://dx.doi.org/10.1016/j.bcp.2016.03.018.
    • Gao, J., Song, J., Du, M., & Mao, X. "Bovine α-lactalbumin hydrolysates (α-LAH) attenuate high-fat diet induced nonalcoholic fatty liver disease by modulating hepatic lipid metabolism in C57BL/6J mice" Journal of Functional Foods 54:254–262 (2019). DOI: 10.1016/j.jff.2019.01.027.
    • Saliba, J., Coutaud, B., Solovieva, V., Lu, F., & Blank, V. "Regulation of CXCL 1 chemokine and CSF 3 cytokine levels in myometrial cells by the MAFF transcription factor" Journal of Cellular and Molecular Medicine 23(4):2517–2525 (2019). DOI: 10.1111/jcmm.14136.
    • ŞEN, A., Ayar, B., Yilmaz, A., ACAR, Ö. Ö., Turgut, G. C., & TOPÇU, G. "Natural diterpenoid alysine A isolated from Teucrium alyssifolium exerts antidiabetic effect via enhanced glucose uptake and suppressed glucose absorption"  Turkish Journal of Chemistry  43(5):1350-1364 (2019).
    • Sun, L., Pang, Y., Wang, X., Wu, Q., Liu, H., Liu, B., … Jiang, C. "Ablation of gut microbiota alleviates obesity-induced hepatic steatosis and glucose intolerance by modulating bile acid metabolism in hamsters" Acta Pharmaceutica Sinica B 9(4):702–710 (2019). DOI: 10.1016/j.apsb.2019.02.004.
    • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).