BlasTaq™ Probe One-Step RT-qPCR

CAT.NOUNITPRICE
G596100 rxn
$115.00

Customize BlasTaq™ Probe One-Step RT-qPCR
1 x BlasTaq™ Probe 2X qPCR MasterMix   + $0.00
1 x RT-qPCR Enzyme Mix   + $0.00
1 x ROX Reference Dye   + $0.00
1 x Nuclease-Free H2O   + $0.00

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BlasTaq™ Probe One-Step RT-qPCR
BlasTaq™ Probe One-Step RT-qPCR

In stock

$115.00

Summary
    Specifications


    Description

    BlasTaqTM Probe One-Step RT-qPCR provides convenience using the RT-qPCR Enzyme Mix and BlasTaqTM Probe 2X qPCR MasterMix for both highly sensitive and specific reverse transcription and TaqMan probe-based real-time qPCR amplification in a single reaction tube. Our proprietary RT-qPCR Enzyme Mix contains stabilizers and enhancers that optimize the two reactions in a “single step”. This offers the end user flexibility with RNA templates and primer selection, an efficient and easy-to-use set up, and a reliable alternative to conventional “two-step” sequential RT-qPCR. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.

     

    Product ComponentQuantity
    BlasTaq™ Probe 2X qPCR MasterMix
    1.25 ml
    RT-qPCR Enzyme Mix
    100 rxn (40 μl)
    ROX Reference Dye 15 μl
    Nuclease-Free H2O
    1.0 ml


    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    SKUG596
    Note

    BlasTaq™ Probe One-Step RT-qPCR comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

    MACHINE TYPEMACHINE
    High ROX Machines
    • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
    • Biometra TOptical
    • Fluidigm BioMark™
    • Wafergene SmartChip System
    • TianLong TL998 System
    Low ROX Machines
    • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
    • BioGene InSyte™
    • Illumina Eco
    • Analytikjena qTower Series
    • Stratagene® Mx3000, Mx3005, Mx4000
    No ROX machines
    • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
    • Roche LightCycler® (480, 1536, Nano)
    • MJ Research Opticon™, Opticon™ 2, Chromo® 4
    • Eppendorf™ Realplex 4
    • BioGene SynChron™
    • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
    • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
    • Cepheid SmartCyler, GeneXpert
    • Enigma™ ML
    • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
    • Qiagen Rotor-Gene™ (Q, 6000)
    • Takara Dice™
    • Thermo Scientific PikoReal
    • DNA-Technology DT96, DTlite, DT-322
    • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
    • Bioneer Exicycler™
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn
    Documents


    Supporting Protocol
    MSDS
    QC

      Other
      FAQs


      References


      13
      • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
      • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
      • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).