BlasTaq™ Probe One-Step RT-qPCR

Cat. No.
G596
Unit
100 rxn
Price
$115.00
Cat. No. G596
Name BlasTaq™ Probe One-Step RT-qPCR
Unit 100 rxn
Category qPCR Mastermixes
Description

(Equivalent to Cat. No. G493-P, G493-PL, G493-PS, G918-P, G918-PL, and G918-PS.)

BlasTaq™ Probe One-Step RT-qPCR provides convenience using the RT-qPCR Enzyme Mix and BlasTaq™ Probe 2X qPCR MasterMix for both highly sensitive and specific reverse transcription and TaqMan probe-based real-time qPCR amplification in a single reaction tube. Our proprietary RT-qPCR Enzyme Mix contains our next generation BlasTaq™ DNA Polymerase as well as stabilizers and enhancers that optimize the two reactions in a “single step”. This offers the end user flexibility with RNA templates and primer selection, an efficient and easy-to-use set up, and a reliable alternative to conventional “two-step” sequential RT-qPCR. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.

Product Features:

  • Simple, one-step RT-qPCR reaction
  • Flexibility with RNA templates and primer selection
  • ROX reference dye provided separately
  • Universally compatible with most qPCR instruments
  • Earn 2X the rewards points

 

ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Product Component Quantity
BlasTaq™ Probe 2X qPCR MasterMix
1.25 ml
RT-qPCR Enzyme Mix
100 rxn (40 μl)
ROX Reference Dye 15 μl
Nuclease-Free H2O
1.0 ml
 

 

Note: This product is abm’s next generation of probe-based One-Step RT-qPCR kits and is functionally equivalent to Cat. No. G493-P, G493-PL, and G493-PS (One-Step TaqProbe qRT-PCR kits) as well as Cat. No. G918-P, G918-PL, and G918-PS (ExCellenCT One-Step TaqProbe qRT-PCR kits), with improved performance. Contact our customer service team [email protected] for more information.


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Storage Condition

Store at -20°C.

Note

BlasTaq™ Probe One-Step RT-qPCR comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE MACHINE
High ROX Machines
  • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
  • Biometra TOptical
  • Fluidigm BioMark™
  • Wafergene SmartChip System
  • TianLong TL998 System
Low ROX Machines
  • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
  • BioGene InSyte™
  • Illumina Eco
  • Analytikjena qTower Series
  • Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines
  • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
  • Roche LightCycler® (480, 1536, Nano)
  • MJ Research Opticon™, Opticon™ 2, Chromo® 4
  • Eppendorf™ Realplex 4
  • BioGene SynChron™
  • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
  • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
  • Cepheid SmartCyler, GeneXpert
  • Enigma™ ML
  • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
  • Qiagen Rotor-Gene™ (Q, 6000)
  • Takara Dice™
  • Thermo Scientific PikoReal
  • DNA-Technology DT96, DTlite, DT-322
  • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
  • Bioneer Exicycler™
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G596
Datasheet
Search CoA here
MSDS
Supporting Protocol
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  • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).
  • Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
  • Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. DOI: 10.1016/j.omtn.2021.05.008
  • Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. DOI: 10.3390/ani11072132
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