qPCR Retrovirus Titer Kit

G949100 rxn

Customize qPCR Retrovirus Titer Kit
1 x BlasTaq™ 2X qPCR Titer MasterMix   + $0.00
1 x Primer Mix   + $0.00
1 x Standard DNA Control   + $0.00
1 x ROX Reference Dye   + $0.00
2 x Nuclease-Free H2O   + $0.00
1 x Virus Lysis Buffer   + $0.00

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qPCR Retrovirus Titer Kit
qPCR Retrovirus Titer Kit

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    abm’s qPCR Retrovirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. MasterMix contains dye comparable to SYBR Green™ and EvaGreen™. 

    Product ComponentQuantity
    BlasTaq™ 2X qPCR Titer MasterMix 1.25 ml
    Primer Mix 100 rxn (200 µl)
    Standard Control DNA 50 µl
    Virus Lysis Buffer 800 µl
    ROX Reference Dye 15 µl
    Nuclease-Free H2O 2 x 1.0 ml
    Unit quantity100 rxn
    Storage Condition

    Store at -20°C


    What is the primer design based on?
    The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
    How come my cells are not successfully infected with the evaluated titer from the kit?
    One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.