qPCR Retrovirus Titer Kit
abm’s qPCR Retrovirus Titer Kit is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and can be completed under one hour. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.
|Unit quantity||100 rxn|
Store at -20°C
|How is the virus lysed?|
The virus is denatured during the initial heating step of the qPCR (i.e., Enzyme Activation, 95°C).
|Since there is no RT incubation step, how is the viral RNA amplified?|
abm's BlasTaq™ 2X qPCR Titer MasterMix has intrinsic RNA-dependent DNA polymerase activity (reverse transcriptase activity), thus eliminating the need to add RTase or an extra RT incubation step.
|What is the primer design based on?|
The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
|How come my cells are not successfully infected with the evaluated titer from the kit?|
One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.