qPCR Retrovirus Titer Kit

CAT.NOUNITPRICE
G949100 rxn
$175.00

Customize qPCR Retrovirus Titer Kit
1 x BlasTaq™ 2X qPCR Titer MasterMix   + $0.00
1 x Primer Mix   + $0.00
1 x Standard DNA Control   + $0.00
1 x ROX Reference Dye   + $0.00
2 x Nuclease-Free H2O   + $0.00
1 x Virus Lysis Buffer   + $0.00

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qPCR Retrovirus Titer Kit
qPCR Retrovirus Titer Kit

In stock

$175.00

Summary
    Specifications


    Description

    abm’s qPCR Retrovirus Titer Kit is a one-step assay which employs a quick RNA extraction step that is followed by RT-qPCR. Designed to deliver high sensitivity and specificity, the kit ensures minimal non-specific background and better overall performance compared to similar kits on the market. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments. MasterMix contains dye comparable to SYBR Green™ and EvaGreen™. 

    Product ComponentQuantity
    BlasTaq™ 2X qPCR Titer MasterMix 1.25 ml
    Primer Mix 100 rxn (200 µl)
    Standard Control DNA 50 µl
    Virus Lysis Buffer 800 µl
    ROX Reference Dye 15 µl
    Nuclease-Free H2O 2 x 1.0 ml

     

    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

     

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    SKUG949
    Unit quantity100 rxn
    Note

    qPCR Retrovirus Titer Kit comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

    MACHINE TYPEMACHINE
    High ROX Machines
    • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
    • Biometra TOptical
    • Fluidigm BioMark™
    • Wafergene SmartChip System
    • TianLong TL998 System
    Low ROX Machines
    • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
    • BioGene InSyte™
    • Illumina Eco
    • Analytikjena qTower Series
    • Stratagene® Mx3000, Mx3005, Mx4000
    No ROX machines
    • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
    • Roche LightCycler® (480, 1536, Nano)
    • MJ Research Opticon™, Opticon™ 2, Chromo® 4
    • Eppendorf™ Realplex 4
    • BioGene SynChron™
    • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
    • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
    • Cepheid SmartCyler, GeneXpert
    • Enigma™ ML
    • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
    • Qiagen Rotor-Gene™ (Q, 6000)
    • Takara Dice™
    • Thermo Scientific PikoReal
    • DNA-Technology DT96, DTlite, DT-322
    • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
    • Bioneer Exicycler™
    Storage Condition

    Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. 

    FAQs


    What is the primer design based on?
    The primers in this kit uses a set of proprietary sequences designed based on the 5'LTR region.
    How come my cells are not successfully infected with the evaluated titer from the kit?
    One important thing to note is that the infection success is dependent on the cell type. Some cells may be more susceptible than others for viral infection. Thus, the same number of infectious units may perform differently on different cell types.
    References


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