BlasTaq™ 2X qPCR MasterMix

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G891500 rxn (4 x 1.25 ml)
$125.00

Customize BlasTaq™ 2X qPCR MasterMix
4 x BlasTaq™ 2X qPCR MasterMix   + $0.00
1 x ROX Reference Dye   + $0.00

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BlasTaq™ 2X qPCR MasterMix
BlasTaq™ 2X qPCR MasterMix

In stock

$125.00

Summary
    Specifications


    Description

    BlasTaq 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

    BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.


    Product Features:

    • Enable streamlined protocol in a simple reaction set-up
    • Allow accurate quantification of a variety of gene targets
    • Reduce pipetting steps to minimize the risk of contamination
    • Compatible with most real-time PCR instruments
    • Contains dye comparable to SYBR Green™ and EvaGreen™ (Probe-based qPCR MasterMix also available)
    • Earn 2X the rewards points

    Request Free Sample

     

    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our qPCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

     

    Product ComponentQuantity
    BlasTaq™ 2X qPCR MasterMix
    500 rxn (4 x 1.25 ml)
    ROX Reference Dye
    50 µl

    Customer Testimonial

    "Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols."

    - Dr. Vivek Vaish, Pune University

     

    BlasTaq DNA Polymerase Testimonial


    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    SKUG891
    Concentration2X
    Note

    BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

    MACHINE TYPEMACHINE
    High ROX Machines
    • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
    • Biometra TOptical
    • Fluidigm BioMark™
    • Wafergene SmartChip System
    • TianLong TL998 System
    Low ROX Machines
    • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
    • BioGene InSyte™
    • Illumina Eco
    • Analytikjena qTower Series
    • Stratagene® Mx3000, Mx3005, Mx4000
    No ROX machines
    • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
    • Roche LightCycler® (480, 1536, Nano)
    • MJ Research Opticon™, Opticon™ 2, Chromo® 4
    • Eppendorf™ Realplex 4
    • BioGene SynChron™
    • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
    • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
    • Cepheid SmartCyler, GeneXpert
    • Enigma™ ML
    • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
    • Qiagen Rotor-Gene™ (Q, 6000)
    • Takara Dice™
    • Thermo Scientific PikoReal
    • DNA-Technology DT96, DTlite, DT-322
    • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
    • Bioneer Exicycler™
    Storage Condition

    Store at -20 °C.

    Unit quantity500 rxn (4 x 1.25 ml)
    FAQs


    I still have additional questions, who do I contact?
    For further details, please email [email protected] or 1-866-757-2414.
    What are the advantages to using BlasTaq™ 2X qPCR MasterMix?
    It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
    References


    25
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    • Liang, Y., Song, X., Li, Y., Su, P., Han, D., Ma, T., … Yang, Q. "circKDM4C suppresses tumor progression and attenuates doxorubicin resistance by regulating miR-548p/PBLD axis in breast cancer" Oncogene 38(42):6850–6866 (2019). DOI: 10.1038/s41388-019-0926-z.
    • Verma, M., Asakura, Y., & Asakura, A. "Inhibition of microRNA‐92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy–related phenotype in mdx mice" Muscle & Nerve 59(5):594–602 (2019). DOI: 10.1002/mus.26433.
    • Wu., Chien-Wei., . "Downregulation of MiR-144 by Triptolide Enhanced p85α−PTEN Complex Formation Causing S Phase Arrest of Human Nasopharyngeal Carcinoma Cells" European Journal of Pharmacology 855:137-148 (2019). DOI: 10.1016/j.ejphar.2019.04.052..
    • Wu, M.-J., Chen, Y.-S., Kim, M. R., Chang, C.-C., Gampala, S., Zhang, Y., … Chang, C.-J. "Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin" Cell Metabolism 29(4):993–1002.e6 (2019). DOI: 10.1016/j.cmet.2018.11.004.
    • Xiang, X., Zhou, Y., Sun, H., Tan, S., Lu, Z., Huang, L., & Wang, W. "Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix" International Immunopharmacology 66:347–353 (2019). DOI: 10.1016/j.intimp.2018.11.035.
    • Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
    • Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.
    • Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. https://doi.org/10.1101/2020.12.20.423533
    • Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. https://doi.org/10.1016/j.omtn.2021.05.008
    • Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. https://doi.org/10.3390/ani11072132