BlasTaq™ 2X qPCR MasterMix
Cat. No. | G891 | |||||||||||
Name | BlasTaq™ 2X qPCR MasterMix | |||||||||||
Unit | 500 rxn (4 x 1.25 ml) | |||||||||||
Category | qPCR Mastermixes | |||||||||||
Description |
(Equivalent to Cat. No. MasterMix-5L, -5R, -5S, -EL, -ER, -ES, KL, -KR, -KS, -LR, -mL, -mR, -mS, -R, and -S) BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.
For a larger size, see Cat. No. G892 (2,500 rxn, 25.0 ml)
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Concentration | 2X | |||||||||||
Storage Condition |
Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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Note |
BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
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Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G891 |
I still have additional questions, who do I contact? | |
For further details, please email [email protected] or 1-866-757-2414.
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Is the dye set-up similar to SYBR Green™ & EvaGreen™? | |
Yes, it is comparable.
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What are the advantages to using BlasTaq™ 2X qPCR MasterMix? | |
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
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- Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
- Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. DOI: 10.1016/j.omtn.2021.05.008
- Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. DOI: 10.3390/ani11072132