BlasTaq™ 2X qPCR MasterMix

G891500 rxn (4 x 1.25 ml)

Customize BlasTaq™ 2X qPCR MasterMix
4 x BlasTaq™ 2X qPCR MasterMix   + $0.00
1 x ROX Reference Dye   + $0.00

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BlasTaq™ 2X qPCR MasterMix
BlasTaq™ 2X qPCR MasterMix

In stock




    (Equivalent to Cat. No. MasterMix-5L, -5R, -5S, -EL, -ER, -ES, KL, -KR, -KS, -LR, -mL, -mR, -mS, -R, and -S)

    BlasTaq 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

    BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.

    Product Features:

    • Enable streamlined protocol in a simple reaction set-up
    • Allow accurate quantification of a variety of gene targets
    • Reduce pipetting steps to minimize the risk of contamination
    • Compatible with most real-time PCR instruments
    • Contains dye comparable to SYBR Green™ and EvaGreen™ (Probe-based qPCR MasterMix also available)
    • Earn 2X the rewards points

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    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our qPCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).


    Product ComponentQuantity
    BlasTaq™ 2X qPCR MasterMix
    500 rxn (4 x 1.25 ml)
    ROX Reference Dye
    50 µl

    Customer Testimonial

    "Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols."

    - Dr. Vivek Vaish, Pune University


    BlasTaq DNA Polymerase Testimonial

    Note: This product is abm’s next generation of dye-based qPCR MasterMixes and is functionally equivalent to BrightGreen 5X qPCR MasterMixes (Cat. No. MasterMix-5L, MasterMix-5R, MasterMix-5S), BrightGreen Express 2X qPCR MasterMixes (Cat. No. MasterMix-EL, MasterMix-ER, MasterMix-ES), KiloGreen 2X qPCR MasterMixes (MasterMix-KL, MasterMix-KR, MasterMix-KS), BrightGreen 2X qPCR MasterMixes (Cat. No. MasterMix-LR, MasterMix-R, MasterMix-S) and BrightGreen miRNA qPCR MasterMixes (MasterMix-mL, MasterMix-mR, MasterMix-mS), with improved performance. Contact our customer service team [email protected] for more information.

    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    Buy PCR Get Free Equipment


    BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

    High ROX Machines
    • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
    • Biometra TOptical
    • Fluidigm BioMark™
    • Wafergene SmartChip System
    • TianLong TL998 System
    Low ROX Machines
    • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
    • BioGene InSyte™
    • Illumina Eco
    • Analytikjena qTower Series
    • Stratagene® Mx3000, Mx3005, Mx4000
    No ROX machines
    • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
    • Roche LightCycler® (480, 1536, Nano)
    • MJ Research Opticon™, Opticon™ 2, Chromo® 4
    • Eppendorf™ Realplex 4
    • BioGene SynChron™
    • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
    • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
    • Cepheid SmartCyler, GeneXpert
    • Enigma™ ML
    • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
    • Qiagen Rotor-Gene™ (Q, 6000)
    • Takara Dice™
    • Thermo Scientific PikoReal
    • DNA-Technology DT96, DTlite, DT-322
    • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
    • Bioneer Exicycler™
    Storage Condition

    Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly.

    Unit quantity500 rxn (4 x 1.25 ml)

    I still have additional questions, who do I contact?
    For further details, please email [email protected] or 1-866-757-2414.
    What are the advantages to using BlasTaq™ 2X qPCR MasterMix?
    It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.