BlasTaq™ 2X qPCR MasterMix

Cat. No.
G892
Unit
2,500 rxn (25.0 ml)
Price
$545.00
Cat. No. G892
Name BlasTaq™ 2X qPCR MasterMix
Unit 2,500 rxn (25.0 ml)
Category qPCR Mastermixes
Description

BlasTaq 2X qPCR MasterMix provides a convenient, reliable and robust setup for performing quantitative real-time analysis of DNA samples. This ready-to-use qPCR MasterMix contains abm’s strategically-engineered, next generation Taq Polymerase, BlasTaq™ DNA Polymerase, providing for rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.

BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. qPCR products made with BlasTaq™ can be used with TA cloning vectors.


Product Features:

  • Enable streamlined protocol in a simple reaction set-up
  • Allow accurate quantification of a variety of gene targets
  • Reduce pipetting steps to minimize the risk of contamination
  • Compatible with most real-time PCR instruments
  • Contains dye comparable to SYBR Green™ and EvaGreen™ (Probe-based qPCR MasterMix also available)
  • Earn 2X the rewards points

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ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our qPCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

 

Product Component Quantity
BlasTaq™ 2X qPCR MasterMix
2,500 rxn (25.0 ml)
ROX Reference Dye
240 µl

 

BlasTaq DNA Polymerase Testimonial

Note: This product is abm’s next generation of dye-based qPCR MasterMixes and is functionally equivalent to Cat. No. MasterMix-LR-XL, MasterMix-R-XL, and MasterMix-S-XL(BrightGreen 2X qPCR MasterMixes), with improved performance. Contact our customer service team [email protected] for more information.


2X the points for PCR Enzymes, Buffers, Solutions, and more!

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Concentration 2X
Storage Condition

Store at -20°C. <span>This product is stable for 2 years from the date of shipping if stored and handled properly.

Note

BlasTaq™ 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE MACHINE
High ROX Machines
  • ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
  • Biometra TOptical
  • Fluidigm BioMark™
  • Wafergene SmartChip System
  • TianLong TL998 System
Low ROX Machines
  • ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
  • BioGene InSyte™
  • Illumina Eco
  • Analytikjena qTower Series
  • Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines
  • BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
  • Roche LightCycler® (480, 1536, Nano)
  • MJ Research Opticon™, Opticon™ 2, Chromo® 4
  • Eppendorf™ Realplex 4
  • BioGene SynChron™
  • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
  • Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
  • Cepheid SmartCyler, GeneXpert
  • Enigma™ ML
  • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
  • Qiagen Rotor-Gene™ (Q, 6000)
  • Takara Dice™
  • Thermo Scientific PikoReal
  • DNA-Technology DT96, DTlite, DT-322
  • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
  • Bioneer Exicycler™
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G892
  • Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
  • Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. DOI: 10.1016/j.omtn.2021.05.008
  • Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. DOI: 10.3390/ani11072132
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