| Description | BlasTaqTM Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaqTM DNA polymerase, MgCl2, and other proprietary buffer components) have been developed for superb and sensitive performance in under one hour. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.
| Product Component | Quantity |
|---|
| BlasTaqTM Probe 2X qPCR MasterMix |
500 rxn (4 x 1.25 ml) |
| ROX Reference Dye |
50 µl |
|
|---|
| Note | BlasTaq™ Probe 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.
| MACHINE TYPE | MACHINE |
|---|
| High ROX Machines |
- ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series
- Biometra TOptical
- Fluidigm BioMark™
- Wafergene SmartChip System
- TianLong TL998 System
|
| Low ROX Machines |
- ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7
- BioGene InSyte™
- Illumina Eco
- Analytikjena qTower Series
- Stratagene® Mx3000, Mx3005, Mx4000
|
| No ROX machines |
- BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™
- Roche LightCycler® (480, 1536, Nano)
- MJ Research Opticon™, Opticon™ 2, Chromo® 4
- Eppendorf™ Realplex 4
- BioGene SynChron™
- Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600)
- Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat)
- Cepheid SmartCyler, GeneXpert
- Enigma™ ML
- Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS
- Qiagen Rotor-Gene™ (Q, 6000)
- Takara Dice™
- Thermo Scientific PikoReal
- DNA-Technology DT96, DTlite, DT-322
- Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680)
- Bioneer Exicycler™
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