BlasTaq™ Probe 2X qPCR MasterMix

Cat. No.

G890

Unit

500 rxn (4 x 1.25 ml)

Price

$135.00

Specifications

Cat. No.

G890

Name

BlasTaq™ Probe 2X qPCR MasterMix

Unit

500 rxn (4 x 1.25 ml)

Category

PCR, qPCR, and RT

Description

BlasTaq™ Probe 2X qPCR MasterMix is designed for TaqMan probe-based real-time qPCR analysis of DNA samples. The components in the MasterMix (dNTPs, BlasTaq™ DNA polymerase, MgCl₂, and other proprietary buffer components) have been developed for superb and sensitive performance in under one hour. The MasterMix is suitable for SNP genotyping assays, gene expression analysis, microarray validation, and high throughput screening applications. ROX reference dye is provided separately, making it universally compatible with most qPCR instruments.

Product Features:

Designed for TaqMan Probe-based qPCR (Dye-based qPCR MasterMix also available)
40-70% less time than regular Taq
6 kb/min extension speed
Highly robust and sensitive
Earn 2X the rewards points
Probe-based One-Step RT-qPCR kit also available (Cat. No. G596)

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Product Component	Quantity
BlasTaq™ Probe 2X qPCR MasterMix	500 rxn (4 x 1.25 ml)
ROX Reference Dye	50 µl

BlasTaq DNA Polymerase Testimonial

Note: This product is abm’s next generation of probe-based qPCR MasterMixes and is functionally equivalent to Cat. No. MasterMix-5P, MasterMix-5PL, MasterMix-5PS, MasterMix-P, MasterMix-PM, and MasterMix-PS (TaqProbe 5X and 2X qPCR MasterMixes), with improved performance. Contact our customer service team [email protected] for more information.

Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Note

BlasTaq™ Probe 2X qPCR MasterMix comes with a separate vial of ROX Reference Dye which can be added depending on the qPCR machine type, as listed in the table below.

MACHINE TYPE	MACHINE
High ROX Machines	ABI ® 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, PRISM™ Sequencing Detection Series Biometra TOptical Fluidigm BioMark™ Wafergene SmartChip System TianLong TL998 System
Low ROX Machines	ABI® 7500, 7500 Fast, Viia™ 7, QuantStudio, QuantStudio 3/5/6/7 BioGene InSyte™ Illumina Eco Analytikjena qTower Series Stratagene® Mx3000, Mx3005, Mx4000
No ROX machines	BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ Roche LightCycler® (480, 1536, Nano) MJ Research Opticon™, Opticon™ 2, Chromo® 4 Eppendorf™ Realplex 4 BioGene SynChron™ Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) Eppendorf Mastercycler® realplex (s, 4 , 4s), Pro (S, 384), Nexus (gradient, eco, flat) Cepheid SmartCyler, GeneXpert Enigma™ ML Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS Qiagen Rotor-Gene™ (Q, 6000) Takara Dice™ Thermo Scientific PikoReal DNA-Technology DT96, DTlite, DT-322 Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) Bioneer Exicycler™

Documents

Supporting Protocol

G890 Datasheet

MSDS

G890 MSDS

Other

FAQs

There are no FAQs for this product yet!

References

Yang, S., Tian, M., & Johnson, A. N. (2020). SARS-CoV-2 protein ORF3a is pathogenic inDrosophilaand causes phenotypes associated with COVID-19 post-viral syndrome. SARS-CoV-2 Protein ORF3a Is Pathogenic in Drosophila and Causes Phenotypes Associated with COVID-19 Post-Viral Syndrome. Published. DOI:10.1101/2020.12.20.423533
Jones, MR et al. "The atm-1 gene is required for genome stability in Caenorhabditis elegans" Mol. Genet. Genomics 287 (4):325-335 (2012). DOI: 10.1007/s00438-012-0681-0. PubMed: 22350747. Application: PCR.
Yang, Y. W., Meng, X., Meng, Y. Y., Tang, H. K., Cheng, M. H., Zhang, Z. Q., Xu, W. Q., & Long, W. (2021). ceRNA regulatory network of FIH inhibitor as a radioprotector for gastrointestinal toxicity by activating the HIF-1 pathway. Molecular Therapy - Nucleic Acids, 25, 173–185. DOI: 10.1016/j.omtn.2021.05.008
Luo, N., Cheng, W., Zhou, Y., Gu, B., Zhao, Z., & Zhao, Y. (2021). Screening Candidate Genes Regulating Placental Development from Trophoblast Transcriptome at Early Pregnancy in Dazu Black Goats (Capra hircus). Animals, 11(7), 2132. DOI: 10.3390/ani11072132

Controls and Related Product:

SafeView™ Classic

CAT.NO

G108

UNIT

1.0 ml

PRICE

$75.00

Safe-Red™ Gel

CAT.NO

G680

UNIT

1.0 ml

PRICE

$85.00

Safe-Red™

CAT.NO

G108-R

UNIT

1.0 ml

PRICE

$50.00

Column-Pure Gel and PCR Clean-Up Kit

CAT.NO

D516

UNIT

100 preparations

PRICE

$165.00

Safe-Green™

CAT.NO

G108-G

UNIT

1.0 ml

PRICE

$45.00

T4 DNA Ligase

CAT.NO

G467

UNIT

200 U (200 μl)

PRICE

$145.00

DNA Purification Magnetic Beads (5 ml)

CAT.NO

G950

UNIT

5 ml

PRICE

$115.00

Pro Ligation-Free Cloning Kit (20 reactions)

CAT.NO

E086

UNIT

20 Reactions

PRICE

$125.00