OneScript® Plus Reverse Transcriptase

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G237100 rxn
$120.00

Customize OneScript® Plus Reverse Transcriptase
1 x OneScript® Plus Reverse Transcriptase   + $0.00
1 x 5X RT Buffer   + $0.00

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OneScript® Plus Reverse Transcriptase
OneScript® Plus Reverse Transcriptase

In stock

$120.00

Summary
    Specifications


    Description

    OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb,  and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.


     

    Product ComponentQuantity
    OneScript® Plus Reverse Transcriptase 100 rxn (100 µl)
    5X RT Buffer 400 µl
     
    SKUG237
    Applications
    • Synthesizing cDNA from a ssRNA
    • DNA primer extension
    • Sequencing dsDNA
    • Constructing cDNA library
    • Constructing libraries for serial analysis for gene expression (SAGE)
    • Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
    • Producing template for use in RT-PCR or real-time RT-PCR
    • Labelling 3'-end of duplex DNA via end-filling reactions
    • Generating probes for hybridization
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn
    Documents


    Supporting Protocol
    MSDS
    QC

      Other

      FAQs


      References


      17
      • Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
      • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
      • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
      • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
      • Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.
      • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
      • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).