OneScript® Plus Reverse Transcriptase

CAT.NO	UNIT	PRICE
G237	100 rxn	$120.00

Specifications

Print Version

Description

(Equivalent to Cat. No. G177, G231, G232, G281, G282, G283, and G284.)

OneScript^® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates.

OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features:

High thermostability at 50-55°C/122-131°F
Synthesize 15 kb RNA Templates
10-15 minutes reaction time
Earn 2X the rewards points
OneScript^® Plus cDNA Synthesis Kit (Cat. No. G236) also available
Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows

ISO 13485:2016 MDSAP Certified
Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Product Component	Quantity
OneScript^® Plus Reverse Transcriptase	100 rxn (100 µl)
5X RT Buffer	400 µl

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Note: This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript^® Two-Step RT-PCR Kits and OneScript^® Plus Two-Step RT-PCR Kits). Contact our customer service team [email protected] for more information.

SKU G237

Applications

Synthesizing cDNA from a ssRNA
DNA primer extension
Sequencing dsDNA
Constructing cDNA library
Constructing libraries for serial analysis for gene expression (SAGE)
Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
Producing template for use in RT-PCR or real-time RT-PCR
Labelling 3'-end of duplex DNA via end-filling reactions
Generating probes for hybridization

Storage Condition

Store at -20°C.

Unit quantity 100 rxn

Documents

Supporting Protocol

G237 OneScript® Plus Reverse Transcriptase Datasheet

MSDS

G237 MSDS

Other

FAQs

References

Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.
Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).