OneScript® Plus Reverse Transcriptase

(Equivalent to Cat. No. G177, G231, G232, G281, G282, G283, and G284.)

OneScript^® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. 

OneScript^® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

Product Features:

• High thermostability at 50-55°C/122-131°F
• Synthesize 15 kb RNA Templates
• 10-15 minutes reaction time
• Earn 2X the rewards points
• OneScript^® Plus cDNA Synthesis Kit (Cat. No. G236) also available
• Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows

ISO 13485:2016 MDSAP Certified Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

Note: This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript® Two-Step RT-PCR Kits and OneScript® Plus Two-Step RT-PCR Kits). Contact our customer service team [email protected] for more information.