OneScript® Plus Reverse Transcriptase

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G237100 rxn
$120.00

Customize OneScript® Plus Reverse Transcriptase
1 x OneScript® Plus Reverse Transcriptase   + $0.00
1 x 5X RT Buffer   + $0.00

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OneScript® Plus Reverse Transcriptase
OneScript® Plus Reverse Transcriptase

In stock

$120.00

Summary
    Specifications


    Description

    (Equivalent to Cat. No. G177, G231, G232, G281, G282, G283, and G284.)

    OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. 

    OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection.

    Product Features:

    • High thermostability at 50-55°C/122-131°F
    • Synthesize 15 kb RNA Templates
    • 10-15 minutes reaction time
    • Earn 2X the rewards points
    • OneScript® Plus cDNA Synthesis Kit (Cat. No. G236) also available
    • Pair with BlasTaq 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows

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    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).

     

    Product ComponentQuantity
    OneScript® Plus Reverse Transcriptase 100 rxn (100 µl)
    5X RT Buffer 400 µl
    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    Buy PCR Get Free Equipment

    Note: This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript® Two-Step RT-PCR Kits and OneScript® Plus Two-Step RT-PCR Kits). Contact our customer service team [email protected] for more information.

     

    SKUG237
    Applications
    • Synthesizing cDNA from a ssRNA
    • DNA primer extension
    • Sequencing dsDNA
    • Constructing cDNA library
    • Constructing libraries for serial analysis for gene expression (SAGE)
    • Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE)
    • Producing template for use in RT-PCR or real-time RT-PCR
    • Labelling 3'-end of duplex DNA via end-filling reactions
    • Generating probes for hybridization
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn
    FAQs


    References


    15
    • Menon, V et al. "The contribution of visceral fat to improved insulin signaling in Ames dwarf mice" Aging Cell 13 (3):497-506 (2014). DOI: 10.1111/acel.12201. PubMed: 24690289. Application: Reverse Transcription.
    • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular networkmodeling" J Physiol. : (2014). PubMed: 25384784.
    • Correnti, JM et al. "Adiponectin fine-tuning of liver regeneration dynamics revealed through cellular network modelling" J Physiol 593(2):365-383 (2015). DOI: 10.1113/jphysiol.2014.284109. PubMed: 25630259. Application: Reverse Transcription.
    • Guo, C., Wang, X., Zhang, H., Zhi, L., Lv, T., Li, M., … Zhu, W. "Structure-based rational design of a novel chimeric PD1-NKG2D receptor for natural killer cells" Molecular Immunology 114:108–113 (2019). DOI: 10.1016/j.molimm.2019.07.009.
    • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).