MegaFi™ One-Step RT-PCR
Simple One Step Reaction, Superior Performance
MegaFiTM One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFiTM Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.
Store at -20°C.
|Unit quantity||100 rxn|
- MegaFi Fidelity DNA Polymerase (Flyer)
- MegaFi Fidelity DNA Polymerase (Performance Data)
- Why choose abm's PCR Enzymes?
- PCR Product Guide (2021)
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- Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
- Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).