MegaFi™ One-Step RT-PCR

G597100 rxn

Customize MegaFi™ One-Step RT-PCR
1 x RT-PCR Enzyme Mix   + $0.00
2 x 2X One-Step RT-PCR Buffer   + $0.00

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MegaFi™ One-Step RT-PCR
MegaFi™ One-Step RT-PCR

In stock




    Simple One Step Reaction, Superior Performance

    MegaFiTM One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFiTM Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.

    Product Features:

    • Enjoy simple, one-step RT-PCR reactions
    • 1300X lower error rates due to our MegaFi Fidelity DNA Polymerase (Cat. No. G896)
    • Extremely thermostable cDNA synthesis at 50-80°C due to our OneScript® Hot Reverse Transcriptase (Cat. No. G593)
    • Excellent protection against common ribonucleases due to our RNaseOFF Ribonuclease Ihibitor (Cat. No. G591)
    • Earn 2X the rewards points


    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).


    Product ComponentQuantity
     RT-PCR Enzyme Mix 100 rxn (400 µl)
    2X One-Step RT-PCR Buffer 2 x 1.25 ml

    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn


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