MegaFi™ One-Step RT-PCR

CAT.NOUNITPRICE
G597100 rxn
$195.00

Customize MegaFi™ One-Step RT-PCR
1 x RT-PCR Enzyme Mix   + $0.00
2 x 2X One-Step RT-PCR Buffer   + $0.00

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MegaFi™ One-Step RT-PCR
MegaFi™ One-Step RT-PCR

In stock

$195.00

Summary
    Specifications


    Description

    MegaFiTM One-Step RT-PCR is an enzyme mix of OneScript® Hot Reverse Transcriptase and MegaFiTM Fidelity DNA Polymerase with RNaseOFF Ribonuclease Inhibitor, gel loading dye, and all other necessary reagents in a single One-Step 2X RT-PCR Buffer for both highly sensitive and specific reverse transcription and high fidelity PCR amplification in a single reaction tube from any RNA template. It provides flexibility in choosing desired primers for use with a proprietary RT-PCR buffer containing stabilizers and enhancers that optimize the two reactions in a “single step”. This kit offers simple, efficient reaction setup, and is a reliable alternative to conventional “two-step” sequential RT-PCR.


    Product ComponentQuantity
     RT-PCR Enzyme Mix 100 rxn (400 µl)
    2X One-Step RT-PCR Buffer 2 x 1.25 ml


    2X the points for PCR Enzymes, Buffers, Solutions, and more!

    SKUG597
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn
    FAQs


    References


    13
    • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).