MegaFi™ Pro Fidelity DNA Polymerase
| Cat. No. | G886 | ||||||||||
| Name | MegaFi™ Pro Fidelity DNA Polymerase | ||||||||||
| Unit | 400 rxn | ||||||||||
| Category | PCR Polymerase | ||||||||||
| Description |
2000X lower error rates, 3X more affordable!MegaFi™ Pro Fidelity DNA Polymerase elevates the current standards for specific, sensitive, robust, and high-fidelity PCR performance. This strategically-engineered polymerase is partnered with a monoclonal antibody which inhibits 5’ → 3’ polymerase activity and 3’ → 5’ exonuclease activity at room temperature allowing for highly specific Hot Start reactions. It has exceptional sensitivity and can amplify even the most difficult templates (compared to the leading competitors). Furthermore, MegaFi™ Pro boasts of its high fidelity and ultralow error rates (over 2,000X less than Taq polymerase, representing the lowest error rate on the market), making it incredibly useful for a variety of PCR applications demanding high fidelity, including Next Generation Sequencing, molecular cloning, or diagnostics. Product Features:
1 Buffer contains 2 mm Mg2+
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| Application |
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| Storage Condition |
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G886 |
| What are the advantages of using a High-Fidelity DNA Polymerase? | |
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MegaFi Pro has high fidelity and high processivity. This means it has low error rates leading to more accurate sequences and longer amplicons leading to a faster reaction time. High fidelity is achieved due to its strong proofreading exonuclease activity, making it the preferred polymerase for all PCR applications requiring greater accuracy or the amplification of difficult templates.
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| What ends will my PCR products have? | |
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Blunt ends, this makes it not suitable for TA cloning
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| What does 3' - 5' exonuclease activity mean? | |
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It is the proofreading activity which digests nucleotides with 3´ hydroxyl groups from the 3´ to 5´ direction. This includes correction of mismatched base pairs and terminal 3´ digestion.
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| How do you determine the fidelity of MegaFi™ Pro? | |
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The fidelity of MegaFi Pro has been tested through Blue-white screening assay.
LacZa gene was amplified using MegaFi™ Pro, NEB's Q5, Vazyme's PhantaMax, and Native Taq. The PCR amplicons were then subcloned into pUC19 vector and plated into a X-gal containing agar plate. Any mutations from PCR will cause the colonies to turn while no mutation will remain blue. Blue and white colonies were counted and the error rate per base was calculated and compared to native Taq.
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