RNaseOFF Ribonuclease Inhibitor
| Cat. No. | G138 | |
| Name | RNaseOFF Ribonuclease Inhibitor | |
| Unit | 4,000 U (100 µl) | |
| Category | Molecular Biology Enzymes and Kits | |
| Description |
Same performance as competing brands, better pricing!RNaseOFF Ribonuclease Inhibitor specifically inhibits common ribonucleases (RNases), including RNase A, B, and C, with high affinity. This enzyme is a useful additive in PCR or RT-PCR as it safe-guards RNA against potential RNase contamination without inhibiting polymerase activity. This robust version of RNase inhibitor has improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitor. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Application |
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| Concentration | 40 U/µl | |
| Storage Condition |
Store at -20°C. |
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| Note |
One unit is defined as the amount of RNaseOFF Ribonuclease Inhibitor that is required to inhibit the activity of 5 ng of RNase A by 50%.
This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G138 |
| Are all components in this product RNase-free? | |
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Yes, all components in the RNaseOFF Ribonuclease Inhibitor is RNase-free.
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| What is the shelf life of this reagent? | |
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1 year upon shipment of the reagent.
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| How many reactions can be performed? | |
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The appropriate number of units per reaction will vary depending on the amount of RNase contamination that is present in the reaction mixture for any given experiment.
One unit of RNaseOFF Ribonuclease Inhibitor will inhibit the activity of 5 ng of RNase by 50%; thus, the more RNase contamination present in the specific reaction, the larger number of units of RNaseOFF that will be required for the inhibition.
As a general guideline, a typical amount of RNaseOFF used would be around 25-50 U per 50 µl reaction; note that this is a general guideline only and the number of units required will vary from case to case.
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| Is DTT required for the RNaseOFF Ribonuclease Inhibitor to function? | |
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No. DTT (dithiothreitol) is used to stabilize enzymes and proteins, and it is not required for the RNaseOFF Ribonuclease Inhibitor to function/inhibit ribonucleases.
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| Does RNaseOff (Cat. No. G138) inhibit RNase R activity? | |
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No, RNAseOff does not inhibit RNase R activity.
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- Del Toro, N., Lessard, F., Tardif, S., Bouchard, J., Bourdeau, V., Ferbeyre, G., & Brakier-Gingras, L. "The retinoblastoma tumor suppressor limits ribosomal readthrough during oncogene induced senescence" bioRxiv 788380: (2019).