Poly(A) Polymerase, E. coli
| Cat. No. | E099 |
| Name | Poly(A) Polymerase, E. coli |
| Unit | 25 U (25 µl) |
| Category | Molecular Biology Enzymes and Kits |
| Description |
Poly(A) Polymerase, E. coli (E-PAP) catalyzes the template independent addition of adenosine residues to the 3’ termini of RNA resulting in a poly(A) tail. Unlike in Yeast, the E. coli enzyme’s activity appears to be sequence independent, requiring no specific recognition sequences. This leads to rapid poly(A) addition at virtually all unprotected 3’ RNA termini regardless of secondary structure. |
| Application |
•Labeling of RNA with ATP or Cordycepin |
| Concentration | 1U/µl |
| Components | Enzyme is supplied with a 10X Reaction Buffer |
| Storage Buffer | 20mM Tris-HCl, 100mM NaCl, 0.1mM DTT, 0.1mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5 @25°C |
| Storage Condition | Store all components at -20°C. |
| Note |
One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C. |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E099 |