miRNA All-In-One cDNA Synthesis Kit

CAT.NOUNITPRICE
G89820 rxn
$180.00

Customize miRNA All-In-One cDNA Synthesis Kit
1 x 2X miRNA cDNA Synthesis SuperMix   + $0.00
1 x Enzyme Mix   + $0.00
1 x Nuclease-Free H2O   + $0.00

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 miRNA All-In-One cDNA Synthesis Kit
miRNA All-In-One cDNA Synthesis Kit

In stock

$180.00

Summary
    Specifications


    Description

    miRNA All-In-One cDNA Synthesis Kit is a complete system for efficient synthesis of first strand miRNA from total RNA templates. This comprehensive kit comes with just two components: 2X miRNA cDNA Synthesis SuperMix and Enzyme Mix, enabling hassle-free set-ups with minimal potential for contamination due to significantly reduced pipetting. The kit contains all miRNA synthesis-required reagents including Poly(A) Polymerase and abm’s OneScript® Plus Reverse Transcriptase, a unique miRNA Oligo d(T) adapter that anneals to the A-tailed miRNA template, and RNaseOFF Ribonuclease Inhibitor to protect RNA template from degradation.

    Product ComponentQuantity
    2X miRNA cDNA Synthesis SuperMix 20 rxn (200 μl)
    Enzyme Mix 20 rxn (40 μl)
    Nuclease-Free H2O 1.0 ml
    SKUG898
    Storage Condition

    Store at -20°C.

    Unit quantity20 rxn
    Documents


    Supporting Protocol
    MSDS
    QC

      Other

      FAQs


      Do I need to purify the sample after adding the poly A tail from the Ambion kit before using the cDNA synthesis kit?
      No, that is not necessary. As per page 4 in the miRNA manual http://www.abmgood.com/miRNA/pdfs/miRNA%20HandbookV11.pdf, the sample can be used directly for the next step in the cDNA synthesis kit.
      How to design individual miRNA primer ?
      The individual miRNA primer could be purchased at http://www.abmgood.com/miRNA-Primer-Sets-Human.html
      Is it possible to use your kit (miRNA cDNA Synthesis Kit) to do quantitative PCR with the Taqman strategy?
      This kit along with our miRNA primers would not be compatible for use with Taqman strategy.
      How does the universal reverse primer work?
      The universal reverse primer can be used for all mature miRNA. To quantify or amplify a specific miRNA, the universal reverse primer with the appropriate forward miRNA primer needs to be used. Our forward primers can work with any kits. Our universal primers will only work with abm's cDNA synthesis kit.
      Can I use SYBR green mastermix for downstream qPCR of the cDNA produced with this kit?
      It may be possible to use a SYBR green mastermix for qPCR, however, our BrightGreen miRNA miRNA qPCR MasterMix recipe has been fully optimized to provide the most successful conditions for qPCR. We have performed extensive testing with minor differences in the composition of miRNA qPCR buffer, this has proven to give very dramatic differences to the results of qPCR. General qPCR mastermixes have more additives to prevent non-specificity and formation of primer dimers and the conditions are often too harsh for the miRNA primers to anneal properly
      Should DNase treatment be performed for the RNA extracted before this procedure?
      You can perform gDNA removal before proceeding with the cDNA synthesis. However, we would recommend using Cat# G488 AccuRT gDNA removal kit. Our product uses proprietary method to removal gDNA and to stop the gDNA removal without using chelating agent such as EDTA and no heating step is involved.
      Can the miRNA cDNA Synthesis Kits also be used for plant miRNAs?
      Yes, these kits can also be used for plant miRNAs.
      Can this kit be used for circulating miRNA or exosomal miRNA?
      Yes, our kit does work with circulating miRNA and exosomal miRNA. Depending on the abundance of the target gene, you may use a lower template amount. E.g. House-keeping genes starting template of 100ng total RNA may be sufficient.
      References


      14
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      • Wu, M.-J., Chen, Y.-S., Kim, M. R., Chang, C.-C., Gampala, S., Zhang, Y., … Chang, C.-J. "Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin" Cell Metabolism 29(4):993–1002.e6 (2019). DOI: 10.1016/j.cmet.2018.11.004.
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      • Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
      • Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.