miRNA All-In-One cDNA Synthesis Kit

Cat. No.
G898
Unit
20 rxn
Price
$180.00
Cat. No. G898
Name miRNA All-In-One cDNA Synthesis Kit
Unit 20 rxn
Category Reverse Transcriptase & RT-PCR
Description

miRNA All-In-One cDNA Synthesis Kit is a complete system for efficient synthesis of first strand miRNA from total RNA templates. This comprehensive kit comes with just two components: 2X miRNA cDNA Synthesis SuperMix and Enzyme Mix, enabling hassle-free set-ups with minimal potential for contamination due to significantly reduced pipetting. The kit contains all miRNA synthesis-required reagents including Poly(A) Polymerase and abm’s OneScript® Plus Reverse Transcriptase, a unique miRNA Oligo d(T) adapter that anneals to the A-tailed miRNA template, and RNaseOFF Ribonuclease Inhibitor to protect RNA template from degradation.

 

Product Component Quantity
2X miRNA cDNA Synthesis SuperMix 20 rxn (200 μl)
Enzyme Mix 20 rxn (40 μl)
Nuclease-Free H2O 1.0 ml

2X the points for PCR Enzymes, Buffers, Solutions, and more!

Buy PCR Get Free Equipment

Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G898
Datasheet
Search CoA here
MSDS
Supporting Protocol
  • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
  • Ramraj, et al. "Serum-circulating miRNAs predict neuroblastoma progression in mouse model of high-risk metastatic disease" Oncotarget 7.14:18605 (2016). DOI: 10.18632/oncotarget.7615. Application: miRNA qPCR Analysis.
  • Liang, Y., Song, X., Li, Y., Su, P., Han, D., Ma, T., … Yang, Q. "circKDM4C suppresses tumor progression and attenuates doxorubicin resistance by regulating miR-548p/PBLD axis in breast cancer" Oncogene 38(42):6850–6866 (2019). DOI: 10.1038/s41388-019-0926-z.
  • Verma, M., Asakura, Y., & Asakura, A. "Inhibition of microRNA‐92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy–related phenotype in mdx mice" Muscle & Nerve 59(5):594–602 (2019). DOI: 10.1002/mus.26433.
  • Wu., Chien-Wei., . "Downregulation of MiR-144 by Triptolide Enhanced p85α−PTEN Complex Formation Causing S Phase Arrest of Human Nasopharyngeal Carcinoma Cells" European Journal of Pharmacology 855:137-148 (2019). DOI: 10.1016/j.ejphar.2019.04.052..
  • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
  • Wu, M.-J., Chen, Y.-S., Kim, M. R., Chang, C.-C., Gampala, S., Zhang, Y., … Chang, C.-J. "Epithelial-Mesenchymal Transition Directs Stem Cell Polarity via Regulation of Mitofusin" Cell Metabolism 29(4):993–1002.e6 (2019). DOI: 10.1016/j.cmet.2018.11.004.
  • Xiang, X., Zhou, Y., Sun, H., Tan, S., Lu, Z., Huang, L., & Wang, W. "Ivabradine abrogates TNF-α-induced degradation of articular cartilage matrix" International Immunopharmacology 66:347–353 (2019). DOI: 10.1016/j.intimp.2018.11.035.
  • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).
  • Zhou, L., Li, P., Zhang, M., Han, B., Chu, C., Su, X., ... & Ma, S. "Carbon black nanoparticles induce pulmonary fibrosis through NLRP3 inflammasome pathway modulated by miR-96 targeted FOXO3a" Chemosphere 125075: (2019).
  • Zhou, Y., Lei, J., Xie, Q., Wu, L., Jin, S., Guo, B., ... & Zhang, J. "Fibrinogen-like protein 2 controls sepsis catabasis by interacting with resolvin Dp5" Science Advances 5(11):eaax0629 (2019).
  • Zhu, R., Xue, X., Shen, M., Tsai, Y., Keng, P. C., Chen, Y., … Chen, Y. "NFκB and TNFα as individual key molecules associated with the cisplatin-resistance and radioresistance of lung cancer" Experimental Cell Research 374(1):181–188 (2019). DOI: 10.1016/j.yexcr.2018.11.022.
This product has no review yet.
Controls and Related Product:

CAT.NO
G108
UNIT
1.0 ml
PRICE
$75.00

CAT.NO
G680
UNIT
1.0 ml
PRICE
$85.00

CAT.NO
G108-R
UNIT
1.0 ml
PRICE
$50.00

CAT.NO
D516
UNIT
100 preparations
PRICE
$175.00

CAT.NO
G108-G
UNIT
1.0 ml
PRICE
$45.00

CAT.NO
G467
UNIT
200 U (200 μl)
PRICE
$152.00

CAT.NO
G950
UNIT
4 x 1.25 ml
PRICE
$120.00

CAT.NO
E086
UNIT
20 Reactions
PRICE
$131.00