All-In-One 5X RT MasterMix

G592100 rxn

Customize All-In-One 5X RT MasterMix
1 x All-In-One 5X RT MasterMix   + $0.00
1 x Nuclease-Free H2O   + $0.00

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All-In-One 5X RT MasterMix
All-In-One 5X RT MasterMix

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    (Equivalent to Cat. No. G451, G452, G453, G454, G485, G486, G490, G492, G590, and G916.)

    Enjoy accurate gDNA-free cDNA synthesis!

    All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.

    Product Features:

    • Easy one-step setup reduces pipetting and sample handling
    • High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
    • Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
    • Removes contaminating gDNA with temperature-sensitive DNase
    • Also includes dNTPs, Oligo (dT)s, and random primers
    • Earn 2X the rewards points

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    ISO 13485:2016 MDSAP Certified

    ISO 13485:2016 MDSAP Certified
    Our RT-PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).


    Product ComponentQuantity
    All-In-One 5X RT MasterMix 100 rxn (400 µl)
    Nuclease-Free H2O 1.0 ml
    2X the points for PCR Enzymes, Buffers, Solutions, and more!
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    Note: This product is abm’s next generation of RT MasterMixes and is functionally equivalent to Cat. No. G485, G486, G490, G492, G590, G916 (5X All-In-One RT MasterMixes with AccuRT Genomic DNA Removal Kit or with ExCellenCT Lysis Kits), and G451, G452, G453, G454 (OneScript® cDNA Synthesis SuperMixes), with improved performance. Contact our customer service team [email protected] for more information.

    • Generation of templates for use in RT-PCR and qRT-PCR
    • cDNA synthesis from ssRNA
    • cDNA library construction
    • Generation of probes for hybridization
    • DNA primer extension
    Storage Condition

    Store at -20°C.

    Unit quantity100 rxn


    • Lee, G et al. "Potential monoclonal antibody therapy for the treatment of ovarian cancer" Ovarian cancer—basic science perspective :385-406 (2012). DOI: 10.5772/1268 . Application: Reverse Transcription.
    • Wu, F., Niu, Z., Zhou, B., Li, P., & Qian, F. "PSMB1 Negatively Regulates the Innate Antiviral Immunity by Facilitating Degradation of IKK-ε" Viruses 11(2):99 (2019). DOI: 10.3390/v11020099.
    • Zhao, G., Zhang, T., Sun, H. J., & Liu, J. "Copper nanoparticles induce zebrafish intestinal defects via endoplasmic reticulum and oxidative stress" Metallomics. : (2019).