2nd Generation Packaging System Mix
Cat. No.
LV003
Unit
200µl
Price
$285.00
| Cat. No. | LV003 |
| Name | 2nd Generation Packaging System Mix |
| Unit | 200µl |
| Description |
For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest, 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G). In general, lentiviral vectors with a wildtype 5' LTR need the 2nd generation packaging system because these vectors require TAT for activation. However, the 2nd generation packaging mix will also support the production of 3rd lentiviral expression vector with a chimeric 5' LTR. All the lentiviral expression vectors marketed by ABM are 3rd generation with a chimeric of RSV promoter upstream of 5'-LTR.
Packaging Mix Components (200ul mix of 2 plasmids) : pLenti-P2A, pLenti-P2B
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| Note |
NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV003 |
Datasheet
| How do I convert a generation 3 lentiviral vector into a vector that can be used by generation 2 packaging system? | |
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All second generation packaging systems can package both second and third generation lentiviral expression vectors.
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| Is it possible to make our own plasmid preparations from the packaging mix and then use them in transfections? | |
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Unfortunately it is not possible to amplify these plasmids as they are provided in a pre-made mixture.
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| Can I use 2nd Generation Packaging System for generation of lentivirus for pLKO.1 ShRNA knockdown vectors? | |
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If you are using lentiviral vectors that are compatible with a second generation packaging system then it should be fine, however, we have not tested this in house.
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| Which system should I use to package my TAT dependent vector? | |
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TAT dependent vectors will need to be packaged using a 2nd generation system (abm Cat LV003). 3rd generation packaging systems (abm Cat. LV053) will not be compatible with this type of vector.
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| What is the titer for viruses made by Cat# LV003 with 293T cells? | |
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For both our 2nd or 3rd generation packaging mixes, the crude viral supernatant (i.e. not concentrated nor purified) titer will be around 10^6IU/ml.
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- Nangami, et al. "Fetuin-A (alpha 2HS glycoprotein) modulates growth, motility, invasion, and senescence in high-grade astrocytomas" Cancer Medicine : (2016). DOI: 10.1002/cam4.940. Application: Knockdown of Cell Line.
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- Zhang, et al. "The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line" Tumour Biology 37.5:5847 (2016). DOI: 10.1007/s13277-015-4405-z. Application: Generation of lentiviral vectors.
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- Gomaa, A., Peng, D., Chen, Z., Soutto, M., Abouelezz, K., Corvalan, A., & El-Rifai, W. "Epigenetic regulation of AURKA by miR-4715-3p in upper gastrointestinal cancers" Scientific reports 9(1):1-11 (2019).
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