dCas9 C-terminal Cloning Vector

CAT.NOUNITPRICE
K01410 µg
$545.00

Specifications


Description

This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal.

SKUK014
SystemLentiviral Vector
Vector Map

pLenti-EF1a-dCas9-Cloning

Unit quantity10 µg
PromoterEF1a
Cas TypeNull Mutant
Cas OriginspCas9
Cas9 ExpressionConstitutive Cas9
Shipping Conditions

Shipped at ambient temperature

Bacterial SelectionKanamycin
FormatVector
AppearanceLiquid
Quality ControlRestriction Enzyme Digest and Sequencing
Shelf Life1 year (when stored at -20°C or below)
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Documents


Supporting Protocol

    MSDS

      QC

        Other

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