dCas9 C-terminal Cloning Vector
This Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. With a target-specific guide RNA (sgRNA), it can bind to a specific region of DNA without cutting it. This vector provides an in-frame downstream multiple cloning site, allowing users to attach any protein of interest to the C-terminus of dCas9. The resulting construct will also carry a C-terminal nuclear localization signal.
|Unit quantity||10 µg|
|Cas Type||Null Mutant|
|Cas9 Expression||Constitutive Cas9|
Shipped at ambient temperature
|Quality Control||Restriction Enzyme Digest and Sequencing|
|Shelf Life||1 year (when stored at -20°C or below)|
|Caution||This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.|
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