3rd Generation Packaging Mix

Cat. No.

LV053

Unit

200 µl

Price

$355.00

Cat. No.

LV053

Name

3rd Generation Packaging Mix

Unit

200 µl

Description

abm’s Third Generation lentiviral packaging plasmid mix offers a high-safety, high-efficiency solution for lentivirus production. This system requires four plasmids for viral particle production:

1) Transfer Plasmid containing your gene of interest.
2) Packaging Plasmid containing structural proteins Gag and Pol.
3) Packaging Plasmid containing structural protein Rev.
4) Envelope Plasmid containing transmembrane glycoprotein VSV-G.

The ready-to-use 3rd Generation Packaging Mix combines optimized Packaging and Envelope Plasmids for high viral titers, superior biosafety and reliable gene delivery performance.

Product Mix Component	Vector Map
pLenti-P3A	Click to view
pLenti-P3B	Click to view
pLenti-P3C	Click to view

Applications

Storage Condition

-20°C or below.

Promotions

Bundle up, save more! These cost-saving bundles combine our best-selling Lentivirus Packaging Mix with reagents for transfection, virus titration and transduction!

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV053

Datasheet

LV053 Datasheet

Search CoA here

MSDS

Plasmid MSDS

Supporting Protocol

Other

	What is the ratio of the three packaging plasmids when packaging via 293 cells?
	The three plasmids are present in equal molarity and in equal ratio.

	Is it possible to propagate the packaging plasmids from this product mix?
	No, it is not possible to grow up the plasmids from the product.

	Which system should I use to package my TAT-dependent vector?
	TAT dependent vectors will need to be packaged using a 2nd generation system (abm Cat LV003). 3rd generation packaging systems (abm Cat. LV053) will not be compatible with this type of vector.

	What is the concentration of your packaging plasmid mixes?
	The packaging plasmid mixes are offered at 0.5 µg/µl.

	What viral titers can be achieved using abm's second and third generation packaging mixes?
	Both our second (Cat. No. LV003) and third (Cat. No. LV053) generation packaging mixes can achieve a titer of 10^6 IU/ml from the crude viral supernatant (without concentration/filtration) using HEK293T cells.

	Is RRE present?
	Yes, it is present after Pol in pLenti-P3A.

	What generation does abm use for their lentiviral expression/transfer vectors?
	All lentiviral expression vectors marketed by abm are third generation with a chimeric RSV promoter upstream of the 5’ LTR.

	What is the difference between the lentivirus second and third generation systems?
	The second generation system uses 3 plasmids (transfer, packaging, envelope), while the third generation uses 4 plasmids (transfer, packaging#1, packaging#2, envelope), separating Rev into its own plasmid for enhanced biosafety.

	Which system is safer, second or third generation?
	The third generation system offers a higher biosafety level due to further separation of viral genes on additional plasmids thus further minimizing recombination risk.

	Are abm’s lentiviral vectors TAT-dependent or TAT-independent?
	All of abm’s expression/transfer lentiviral vectors are TAT-independent.

	What cell line should I use for lentivirus production?
	We recommend using HEK293T cell line due to its high transfection efficiency and virus yield.

Do you recommend using second or third generation lentivirus packaging mix?

abm's lentiviral vectors are compatible with second and third generation packaging systems. We recommend the Second Generation Packaging Mix (Cat. No. LV003) for users who want to achieve higher titer lentivirus as only 3 plasmids are required for virus production. We also recommend the Third Generation Packaging Mix (Cat. No. LV053) for users concerned with virus biosafety, as the viral packaging elements are further split into 4 plasmids thus reducing probably of recombination into wild type virus.

Is 2nd Generation or 3rd Generation packaging mix better for lentivirus production?

The 2^nd Generation Packaging System Mix is recommended unless your facility requires 3^rd Generation Packaging Systems for stricter safety regulations.

Feature	2^nd Generation Packaging System Mix	3^rd Generation Packaging System Mix
Transfer Plasmid	Can package 2^nd or 3^rd Generation transfer plasmids (Tat dependent or independent)	Can ONLY package 3^rd Generation transfer plasmids (Tat independent)
Packaging Plasmids	One plasmid encoding Gag, Pol, Rev, and Tat	Two plasmids: one encoding Gag and Pol, and one encoding Rev
Envelope Plasmid	Interchangeable: usually encodes for VSV-G	Interchangeable: usually encodes for VSV-G
Safety	Safe. Replication incompetent: Uses 3 separate plasmids encoding various HIV genes.	Safer. Replication incompetent and always self inactivating: Uses 4 plasmids instead of 3 and eliminates the requirement for Tat.
Recombinant Virus Yield	Higher yields than 3^rd Generation due to fewer plasmids (3) required to be transfected	Lower yields than 2^nd Generation due to more plasmids (4) required to be transfected

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