RNA Purification Magnetic Beads
| Cat. No. | G971 |
| Name | RNA Purification Magnetic Beads |
| Unit | 5 ml |
| Description |
abm’s RNA Purification Magnetic Beads are paramagnetic particles coated with carboxyl groups that can reversibly bind to nucleic acids. The RNA magnetic separation beads are specifically formulated for usage with RNA and can be used to concentrate and purify samples resulting in reproducible, high yield and purity. Key Features:
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| Application |
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| Storage Condition | Store tightly sealed at 4°C. Do not freeze. |
| Note |
Bring magnetic beads to room temperature 30 min before use, vortex to thoroughly mix and resuspend. A magnetic separation rack is required (not included). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G971 |
| Can the DNA or RNA Purification Magnetic Beads be reused? | |
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No, the DNA or RNA Purification Magnetic Beads are intended for single-use only, particularly for RNA work where the contamination risk is high.
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| What are the advantages of using paramagnetic beads over silica-based spin columns for nucleic acid purification and concentration? | |
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Advantages include: automation compatible, scalable, flexible sample volumes, size selection capability, no centrifugation equipment required and superior reproducibility.
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| How do abm's DNA and RNA Purification Magnetic Beads work? | |
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Nucleic acids bind to the magnetic beads in the presence of a crowding agent and optimized buffer. Using a magnetic rack, the nucleic acid-bound beads are captured, washed to remove contaminants, and then the purified nucleic acids are eluted under aqueous conditions.
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| Are abm's DNA and RNA Purification Magnetic Beads compatible with automation? | |
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Yes. The provided Datasheets outline a manual workflow, however they are amendable to high-throughput and automated workflows.
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| My yield is lower than expected, what can I do to improve sample recovery when using abm's DNA or RNA Purification Magnetic Beads? | |
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1. Prior to use, ensure beads have come to room temperature (20-25°C) for at least 30 min, then vortex thoroughly until the solution is fully homogenous. 2. Ensure the correct binding ratio is utilized. 3. Check sample integrity prior to purification. 4. Use freshly prepared 70% or 80% ethanol (according to product Datasheet). 5. During the elution step, ensure beads are thoroughly resuspended and incubated with water or elution buffer. |
- Iturralde Martinez, J. F., & Rosa, C. (2023). Reverse transcriptase recombinase polymerase amplification for detection of tomato spotted wilt orthotospovirus from crude plant extracts. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-35343-w