Lentiviral Vectors & Lentivirus
As a leader in lentiviral technology, abm offers an affordable, comprehensive collection of human, mouse and rat genes cloned into ready-to-use lentiviral vectors or packaged recombinant lentivirus for gene over-expression studies within a wide range of host cells. Can't find the lentivirus that you're looking for? Contact our team for a custom construct.
Search our Ready-to-Use Libraries: select the dropdowns and enter gene name, symbol or accession number
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Advantages of the Lentivirus System:
- Large insert capacity (up to 9kb)
- High infection efficiency
- Broad host range (dividing, non-dividing, stem cells, and primary cells)
- Stable integration & expression of recombinant proteins
- Ideal for the creation of stable cell lines
Additional Information
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Achieve Up To 300 Fold Over-Expression
qPCR amplification of OVOL1 gene following transfection with abm's OVOL1 Lentiviral Vector. -
Lentivirus Introduction
Learn more about the Lentivirus system by visiting our Learning Resources.
Documents
Top Publications
| 01 | Histone demethylase KDM5D upregulation drives sex differences in colon cancer. Li J. et al. Nature (2023) doi: 10.1038/s41586-023-06254-7 |
| 02 | Matrix mechanics and water permeation regulate extracellular vesicle transport. Lenzini S. et al. Nature Nanotechnology (2020) doi: 10.1038/s41565-020-0636-2 |
| 03 | Age-related Huntington’s disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy. Oh YM. et al. Nature Neuroscience (2022) doi: 10.1038/s41593-022-01185-4 |
FAQs
| Which packaging system should I use, 2nd or 3rd generation? |
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All of abm's lentviral vectors are compatible with 2nd and 3rd generation packaging systems LV003 and LV053 available from abm . Please note, only packaging mixes produced by abm have been tested in house and therefore carry our guarantee for high titer virus production. If it is desirable to use other packaging plasmids obtained from a different source, the compatibility must be tested and determined by the end user.
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| What is in your expression-ready, genome-wide cDNA collection? |
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Our expression-ready, genome-wide cDNA collection is a curated library of full-length ORF clones covering thousands of genes across the genome. These clones are pre-optimized for high-level gene expression in mammalian systems, making them ideal for functional genomics, overexpression studies, and high-throughput screening.
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| What are lentiviral ORF clones and how are they used? |
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Lentiviral ORF clones are open reading frame (ORF) sequences cloned into lentiviral vectors for stable gene overexpression in target cells without regulatory elements interfering. They are commonly used for functional studies, pathway analysis, and validating gene targets in both dividing and non-dividing cells. Constructs can be customized to include different promoters, reporters, tags, and selection markers.
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| Why use lentiviral expression vectors for gene studies? |
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Lentiviral expression vectors enable efficient and stable gene delivery into a wide range of cell types, including hard-to-transfect primary cells and stem cells. Because lentiviruses integrate into the host genome, these vectors are ideal for long-term gene expression studies, functional screening, and disease modeling.
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| Are your lentiviral clones expression-ready? |
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Yes, our lentiviral clones are expression-ready vectors with strong promoters, optimized Kozak sequences, and selectable markers. This allows direct plasmid transfection or virus packaging and transduction into target cells without additional cloning steps.
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| Can I use lentiviral ORF clones for high-throughput screening? |
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Absolutely. Lentiviral ORF clones are designed for genome-wide functional screening in stable cell lines. Their ability to infect both dividing and non-dividing cells makes them particularly valuable for drug discovery and gene function analysis.
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| What cell types can be transduced with lentiviral cDNA expression vectors? |
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Lentiviral expression vectors can efficiently transduce a wide range of cell types, including:
• Primary cells (e.g., neurons, T cells) • Stem cells • Hard-to-transfect lines (e.g., suspension cells, hematopoietic cells) This broad tropism makes them highly versatile for research and therapeutic applications. |