Safe-Red™
| Cat. No. | G108-R |
| Name | Safe-Red™ |
| Unit | 1.0 ml |
| Category | Gel Documentation |
| Description |
abm’s Safe-Red™ is a safe, high-performance DNA gel stain that can replace ethidium bromide in agarose gel electrophoresis. This non-toxic EtBr alternative eliminates the contamination risks to glassware, gel apparatus and the environment while offering sensitive and reliable nucleic acid staining & visualization. Features:
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| Safeview Series | Loading Dye |
| LED Viewer Compatibility | Yes |
| Stain Color | Red |
| Application |
Safe Detection of dsDNA, ssDNA and RNA in agarose gel electrophoresis. |
| Concentration | 6X |
| Format General | Loading Dye |
| Storage Condition |
Store at 18-25°C. |
| Note |
Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108-R |
| How sensitive are SafeView products? | |
|
Safe-Green™: 0.2-0.6 ng DNA per band |
| I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do? | |
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It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.
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| Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel? | |
|
SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.
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| What color is the tracking dye of Safe-Green and Safe-Red during electrophoresis? | |
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Safe-Green™ and Safe-Red™ both contain a dark blue tracking dye that migrates at ~300bp on a 1% agarose gel. Safe-Red™ contains an additional light blue tracking dye that migrates at ~4000bp on a 1% agarose gel.
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| What type of device or imaging system should be used to visualize SafeView agarose gels? | |
|
We recommend using abm’s SafeViewER™ Imager (Cat. No. E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.
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| Can SafeView products be used in replacement of ethidium bromide? | |
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All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow.
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| How do SafeView products work and why are they not carcinogenic? | |
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SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide.
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| How do I use SafeView products? | |
|
Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel. SafeView™ Classic and Safe-Red™ Gel are supplied in a 10,000X format: for pre-cast, add 10µl SafeView™ Classic or Safe-Red™ Gel per 100ml molten agarose, mix gently and cast the gel; for post-stain, prepare and run an unstained agarose gel, next submerge the gel in post-staining solution of 30µl SafeView™ Classic or Safe-Red™ Gel per 100ml of 1X TAE or 1X TBE buffer for 30min in the dark with light agitation, then image accordingly. |
| Can SafeView products differentiate between double stranded and single stranded nucleic acids? | |
|
No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.
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| At what temperature do I store SafeView products? | |
|
SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C.
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| Can SafeView products be used to post-stain gels? | |
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Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.
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| Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel? | |
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If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis.
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| What is the concentration of SafeView products? | |
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SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.
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| Can I perform gel extraction and subsequent cloning using SafeView products? | |
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We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.
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| I'm seeing a "dark zone" at the bottom of my gel that is making it difficult to see faint bands. How can I fix this? | |
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This type of pattern is typically associated with variations in staining distribution or illumination during imaging rather than a limitation of the stain itself. To help troubleshoot the issue, we recommend checking the following points:
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