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SafeView™ Classic

Cat. No.

G108

Unit

1.0 ml

Price

$78.00

Cat. No.	G108
Name	SafeView™ Classic
Unit	1.0 ml
Category	Gel Documentation
Description	abm’s SafeView™ Classic is a safe, high-performance DNA gel stain that fully replaces ethidium bromide in agarose gel electrophoresis. This non-toxic EtBr alternative eliminates the contamination risks to glassware, gel apparatus and the environment while offering sensitive and reliable nucleic acid staining & visualization. Features: Easy to Use: Compatible with UV and blue light. Safe: Non-carcinogenic. Sensitive: Detect as little as 1-2 ng of DNA per gel band. Stable: Store at room temperature (18-25°C). Superior: Compatible with downstream applications such as cloning and sequencing.
Safeview Series	Gel Casting Dye
LED Viewer Compatibility	Yes
Stain Color	Green
Application	Safe detection of dsDNA, ssDNA and RNA in agarose gel electrophoresis.
Concentration	10,000X in DMSO
Format General	Pre-cast or Post-stain
Storage Condition	Store at 18-25°C for up to 2 years.
Note	Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108

Datasheet

SafeView™ Classic Datasheet

Search CoA here

MSDS

SafeView™ Classic MSDS

Other

	How sensitive are SafeView products?
	Safe-Green™: 0.2-0.6 ng DNA per band Safe-Red™: 0.6-1.0 ng DNA per band Safe-Red™ Gel: 0.6-1.0 ng DNA per band SafeView™ Classic: 1.0-2.0 ng DNA per band

How do I dispose of used gloves, electrophoresis buffer and agarose gels stained with SafeView?

Dispose of SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (e.g. Acridine orange, Propidium iodide). All gels and contaminated “non-sharp” lab debris (e.g., gloves, pads, towels, tubes, etc.) that are processed using this stain can be discarded in regular landfill trash. Used electrophoresis buffer and staining solutions that contain the stain can either be collected and disposed of through the HWMU or collected and run through an approved filter device. The buffer solutions that have been run through the approved filter should be checked under the appropriate light source for complete removal of the dyes, and if it passes (does not fluoresce), the liquid can be disposed of down the drain with a copious amount of water as long as no other materials are present that would cause the material to be a Hazardous Waste. The filters that have been used up and are no longer effective must be disposed of through the HWMU.

	I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do?
	It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.

	Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel?
	SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.

	Can SafeView Classic stain penetrate cell membranes?
	Yes, SafeView™ Classic can penetrate living cell membranes.

	What type of device or imaging system should be used to visualize SafeView agarose gels?
	We recommend using abm’s SafeViewER™ Imager (Cat. No. E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.

	Can SafeView products be used in replacement of ethidium bromide?
	All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow.

How do SafeView products work and why are they not carcinogenic?

SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide.

How do I use SafeView products?

Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel.

SafeView™ Classic and Safe-Red™ Gel are supplied in a 10,000X format: for pre-cast, add 10µl SafeView™ Classic or Safe-Red™ Gel per 100ml molten agarose, mix gently and cast the gel; for post-stain, prepare and run an unstained agarose gel, next submerge the gel in post-staining solution of 30µl SafeView™ Classic or Safe-Red™ Gel per 100ml of 1X TAE or 1X TBE buffer for 30min in the dark with light agitation, then image accordingly.

	Can SafeView products differentiate between double stranded and single stranded nucleic acids?
	No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.

	At what temperature do I store SafeView products?
	SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C.

	Can SafeView products be used to post-stain gels?
	Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.

	Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel?
	If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis.

	What is the concentration of SafeView products?
	SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.

	Can I perform gel extraction and subsequent cloning using SafeView products?
	We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.

I'm seeing a "dark zone" at the bottom of my gel that is making it difficult to see faint bands. How can I fix this?

This type of pattern is typically associated with variations in staining distribution or illumination during imaging rather than a limitation of the stain itself.

To help troubleshoot the issue, we recommend checking the following points:

Mixing of the stain in molten agarose
When adding SafeView Classic to the agarose, please ensure the solution is thoroughly mixed before pouring the gel. If the agarose cools significantly before pouring, the dye may distribute unevenly.
Gel casting conditions
Make sure the casting tray is on a level surface so the gel thickness is uniform across the entire gel.
Imaging conditions
If using a gel documentation system, please try capturing the image with automatic background correction or normalization disabled, as these settings can sometimes introduce gradients in the image.
Transilluminator check
Ensure the gel is centered on the transilluminator and that the surface is clean. Uneven illumination from the light source can also produce similar patterns.
Running buffer and Voltage
Using fresh running buffer (e.g., TAE or TBE) and ensuring the voltage is not too high can help with consistent migration and signal. If the bottom of the gel overheats or experiences buffer ion depletion, migration and staining of small fragments can be affected.

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