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Applied Biological Materials Inc.
Calciumfectin™
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Data Sheet
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Calciumfectin™ Mammalian Transfection Reagent Kit
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Cat.No.: G099
Quantity: Kit (100 transfections)
Price: $105.00
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Phone: 604-247-2416 (Local), 1-866-757-2414 (Toll free)
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Data Sheet
Description The Calciumfectin™ Mammalian Transfection Reagent Kit provides high-quality reagents suitable for both transient and stable transfections of mammalian cells.
Application The kit was especially developed for high titer recombinant retrovirus production.
Protocol Click To Open
Kit Contents 6 ml 2 M Calcium Solution 50 ml 2X HEPES-Buffered Saline (HBS) 70 ml Sterile H2O
Storage Store HBS buffer at 20ºC; all other components at 4ºC.
Notes This product is distributed for laboratory research only. Caution: Not for diagnostic use .
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Does freeze-thaw affect the performance of the transfection reagent?
Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss.

If possible, test the agent with a GFP control first.