Mouse Monoclonal Antibody Production

  Key requirements for successful monoclonal antibody generation include immunization strategies, antigen design, and screening technologies. ABM's novel immunization procedures and careful mouse strain selection allows for production of monoclonal antibodies which are of the utmost affinity and specificity. This is because we take great care in designing the antigens for antibody production, especially with respect to synthesizing peptides, to generate a robust antibody that is able to recognize conformational changes of the native protein under the optimum number of conditions.
  Mouse monoclonal antibody production is divided into different phases, with a non-refundable $1,000 to cover the material cost to initiate the project. The balance will be due upon service completion ($2200). Our price includes antigen protein production and guaranteed western blot results, which are not included in competitor's services. All you need to do is give us the gene accession number and we will take care of everything else. This unique service is only available from abm.

To order, check the corresponding box(es) on the right, and then click 'Add to Cart'.

Service	Description	Cat. No.	Price
Complete Package	Recombinant protein production Immunization (5 mice/antigen) and Sera Testing by ELISA Fusion and Colony Screening Subcloning, Colony Expansion and Freezing Guaranteed western blot positive clones (2 clones) Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)	C080	$3200.00
Package without protein production	Immunization (5 mice/antigen) and Sera Testing by ELISA Fusion and Colony Screening Subcloning, Colony Expansion and Freezing Guaranteed western blot positive clones (2 clones) Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)	C087	$2000.00
Additional Clone(s)	Steps 4 and 5	C081	$500.00 per Clone
Ascites Production	Production from 1-25 Mice	C082	$15.00 per Mouse
	Production from 26-50 Mice	C083	$12.50 per Mouse
	Production from 51-100 Mice	C084	$10.00 per Mouse
	Production from 101-150 Mice	C085	$9.50 per Mouse
	Production from 151-200 Mice	C086	$9.00 per Mouse
	Production from 200+ Mice	Inquire	$8.00-22.50 per Mouse	Inquire

The customer can provide a peptide antigen or a ready-to-inject antigen for monoclonal antibody production. When using a peptide antigen supplied by the customer, an additional charge of $200.00 is applied for KLH conjugation.

Catalog Number	Quantity	Promotion Code (Optional)

Custom Polyclonal Antibody Production

  ABM offers the most compeptitive price for quality, custom, polyclonal antibody production. Our services include peptide synthesis, immunization of 2 rabbits, and peptide affinity purification of positive serum. It is all included and there is absolutely no hidden additional cost. Based on our experience, the success rate of generating a quality polyclonal antibody is over 70% with one peptide antigen and over 95% for two peptide antigen.

To order, check the corresponding box(es) on the right, and then click 'Add to Cart'.

Service	Description	Cat. No.	Price
Standard Service	Customer provides antigens (5mg of >90% purity peptide or 5mg recombinant protein). KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation). ELISA assay for titer until it reaches 1:10,000 or better. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step. The project will be finished in 12 weeks.	C090	$950.00 per antigen
Complete Service	Customer provides gene name or gene/protein sequence. Pepetide is designed and synthesized. KLH conjugation and immunization of 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation). ELISA assay for titer until it reaches 1:10,000 or better. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step. The project will be finished in 14 weeks.	C091	$1250.00 per antigen

What assay do you use to confirm the functionality of the produced antibody?

We use Western blotting to ensure the produced antibody have affinity and specificity towards the antigen, and this service is included in the antibody production service. Immunohistochemistry and Immunoprecipition tests are also available, however are NOT included in the production service.

What is the shortest length peptide that you can produce an antibody against?

To produce an antibody that has specificity and affinity at acceptable levels, we require a minimum of 20 amino acids.

Can you produce an antibody against a native protein?

Yes, however to guarantee the antibody is effective against the native protein purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we can not guarantee these will be effective against the native protein due to folding and other conformational issues. We only guarantee that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.

What is the isotype of antibody your production produces?

Mainly our process results in IgG2a and IgG2b antibodies.

What concentration and amount of antigen is required for production if I am supplying the protein/peptide?

For peptide antigens we require at least 2mg, while if it is a protein 500ug/mouse with concentrations of 0.5-1.0mg/ml are acceptable.

Do you deliver the Hybridoma cells along with the custom antibody?

Yes, the customer will receive the custom hybridoma cells along with the custom antibody.