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PCR General Protocols
Basic PCR Protocol
Components Volume Final Conc.
10X PCR buffer minus Mg 10ul 1X
10 mM dNTP mixture 2ul 200 uM each
50 mM MgCl2 3ul 1.5 mM
Primer mix (10uM each) 4ul 0.4 uM each
Template DNA 1-20ul 100 ng
Taq DNA polymerase (5U/ul) 2.0ul 10U
ddH2O to 100ul n/a
We recommend preparing a master mix to minimize reagent loss and enable accurate pippetting when setting up multiple PCR reactions.
PCR Using Taq
Component Volume
DNA Template 1ul
Primer mix (10uM each) 2ul
PCR MasterMix 12.5ul
ddH2O 9.5ul