Protocols

FAQs

PCR General Protocols

• Basic PCR Protocol
• PCR Using Taq

Basic PCR Protocol

Components	Volume	Final Conc.
10X PCR buffer minus Mg	10ul	1X
10 mM dNTP mixture	2ul	200 uM each
50 mM MgCl2	3ul	1.5 mM
Primer mix (10uM each)	4ul	0.4 uM each
Template DNA	1-20ul	100 ng
Taq DNA polymerase (5U/ul)	2.0ul	10U
ddH2O	to 100ul	n/a

We recommend preparing a master mix to minimize reagent loss and enable accurate pippetting when setting up multiple PCR reactions.

PCR Using Taq

Component	Volume
DNA Template	1ul
Primer mix (10uM each)	2ul
PCR MasterMix	12.5ul
ddH2O	9.5ul