iPSC Factor Lentiviruses
To date, lentiviral vectors have been the most widely used delivery
vehicle for iPSC reprogramming due to its high efficiency of both transduction
and stable integration. As a world leader in iPSC and stem cell research,
abm provides the most comprehensive selection of iPSC lentivral vectors
with choices of different promoters, including, EF1α, PGK, UBC,
CMV. For stable gene expression in stem cells, EF1α promoter offers
highest percentage of cells expressing transgene, follow by PGK and
UBC. However, PGK promoter offers higher gene expression level in positive
cells. For transient gene expresion, CMV promoter is the choice for
higher level transgene expression.
To further simplify the iPSC induction, scientists at ABM have successfully
developed a polycistonic iPSC lentivirus which contains all four iPSC
transcription factors in both Thomson (OSNL) and Yamanaka (OSKM) Protocol
formats. All polycistronic lentiviral vectors are designed with the
popular EF1α promoter.