- miRNA/microRNA
- miRNA-microRNA Expression
- • Cumate Inducible miRNA Lentivirus/Vector
- • GFP miRNA Lentivirus/Vector
- • miRNA Lentivirus/Vector
- • miRNA(microRNA) Adenovirus
- • miRNA Inhibitor Adenovirus
- • miRNA Inhibitor Lentivirus/Vector
- • 3'UTR miRNA Reporter & Cell Line
- miRNA-microRNA Detection
- Custom miRNA-microRNA Services
- miRNA-microRNA Database Access
- miRNA-microRNA FAQs
miRNA FAQs
Back We have compiled a list of commonly asked questions about our miRNA products and services. If your question is not covered here please contact us to technical@abmgood.com.
miRNA FAQs
- What is the Sanger miRBase Sequence Database?
- When was the latest update of your array sequences?
- Do you sell any products that allow detection of microRNAs using in-situ methods
- Do you have any mutant constructs available for the 3'UTR miRNA reporter vectors for miRNA-mRNA interaction validation?
- Do I need to use an internal control primer for quantitative detection of miRNAs (the same as housekeeping primers which we use in qPCR for mRNAs detection)?
- How does miRNA inhibitor adenovirus work?
- Can I quantify mature miRNA in total RNA using your cDNA synthesis and kit and qPCR master mix?
- Which genes are targeted by a specific miRNA?
What is the Sanger miRBase Sequence Database?
miRBase is a sequence database that has been established by the Sanger Institute. Each entry in the microRNA Registry represents a predicted hairpin portion of a microRNA transcript (termed mir in the database), with information on the location and sequence of the mature microRNA sequence (termed miR). The database provides microRNA gene hunters with unique names for novel microRNA genes prior to publication of results and a searchable database of published microRNA.
When was the latest update of your array sequences?
The content of our arrays has been updated to miRBase Release 16.0.
Do you sell any products that allow detection of microRNAs using in-situ methods
We currently don't have in-situ miRNA detection methods.
Do you have any mutant constructs available for the 3'UTR miRNA reporter vectors for miRNA-mRNA interaction validation?
No, we don't have any mutant constructs. All the constructs are wild type.
Do I need to use an internal control primer for quantitative detection of miRNAs (the same as housekeeping primers which we use in qPCR for mRNAs detection)?
For human miRNA plates, the controls are as follows:
1) SNORD44, cat MPH00003
2) SNORD47, cat MPH00004
3) SNORD48, cat MPH00005
4) U6-2, cat MPH00001
1) SNORD44, cat MPH00003
2) SNORD47, cat MPH00004
3) SNORD48, cat MPH00005
4) U6-2, cat MPH00001
How does miRNA inhibitor adenovirus work?
The miRNA inhibitor is in the form of a short hairpin which is complementary to the mature miRNA sequence that it targets. Its expression is under the control of the H1 promoter in an Adenovirus vector. The packaged adenovirus can be used to transfect your target cells and deliver the miRNA inhibitor into the cells and knockdown its target mature miRNA.
Can I quantify mature miRNA in total RNA using your cDNA synthesis and kit and qPCR master mix?
Yes, that is what it designed for.
Which genes are targeted by a specific miRNA?
You can search for predicted and validated miRNA target genes at http://mirbase.org/.
Just type in your miRNA name (eg. hsa-mir-145) or accession number in the search bar and look for the targeted genes.
Just type in your miRNA name (eg. hsa-mir-145) or accession number in the search bar and look for the targeted genes.