- miRNA/microRNA
- miRNA-microRNA Expression
- • GFP miRNA Lentivirus/Vector
- • miRNA Lentivirus/Vector
- • miRNA(microRNA) Adenovirus
- • miRNA Inhibitor Adenovirus
- • miRNA Inhibitor Lentivirus/Vector
- • 3'UTR miRNA Reporter & Cell Line
- miRNA-microRNA Detection
- Custom miRNA-microRNA Services
- miRNA-microRNA Database Access
- miRNA-microRNA FAQs
miRNA cDNA Synthesis
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Data Sheet
| Description | The miRNA cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand miRNA from total RNA templates. The kit utilizes a mutated recombinant M-MuLV Reverse Transcriptase which exhibits limited RNase H activity. The recombinant RNasin™ Ribonuclease Inhibitor, supplied with the kit effectively protects the RNA template from degradation. The kit is also supplied with unique miRNA d(T)adapter. It anneals selectively on the poly(A) tail of miRNA and universal primers in miRNA profiling kit (MA003, MA004). Therefore, they can be used for transcription of the 5'-end regions of miRNA. The first strand of miRNA can be directly used as a template for the qPCR-based analysis of miRNA expression in a given sample. |
| Protocol |  |
| Storage | -20°C |
| Shelf Life | 2 years |
FAQs
- What is the Sanger miRBase Sequence Database?
- When was the latest update of your array sequences?
- Do you sell any products that allow detection of microRNAs using in-situ methods
- Do you have any mutant constructs available for the 3'UTR miRNA reporter vectors for miRNA-mRNA interaction validation?
- Do I need to use an internal control primer for quantitative detection of miRNAs (the same as housekeeping primers which we use in qPCR for mRNAs detection)?
- How does miRNA inhibitor adenovirus work?
What is the Sanger miRBase Sequence Database?
miRBase is a sequence database that has been established by the Sanger Institute. Each entry in the microRNA Registry represents a predicted hairpin portion of a microRNA transcript (termed mir in the database), with information on the location and sequence of the mature microRNA sequence (termed miR). The database provides microRNA gene hunters with unique names for novel microRNA genes prior to publication of results and a searchable database of published microRNA.
When was the latest update of your array sequences?
The content of our arrays has been updated to miRBase Release 16.0.
Do you sell any products that allow detection of microRNAs using in-situ methods
We currently don't have in-situ miRNA detection methods.
Do you have any mutant constructs available for the 3'UTR miRNA reporter vectors for miRNA-mRNA interaction validation?
No, we don't have any mutant constructs. All the constructs are wild type.
Do I need to use an internal control primer for quantitative detection of miRNAs (the same as housekeeping primers which we use in qPCR for mRNAs detection)?
For human miRNA plates, the controls are as follows:
1) SNORD44, cat MPH00003
2) SNORD47, cat MPH00004
3) SNORD48, cat MPH00005
4) U6-2, cat MPH00001
1) SNORD44, cat MPH00003
2) SNORD47, cat MPH00004
3) SNORD48, cat MPH00005
4) U6-2, cat MPH00001
How does miRNA inhibitor adenovirus work?
The miRNA inhibitor is in the form of a short hairpin which is complementary to the mature miRNA sequence that it targets. Its expression is under the control of the H1 promoter in an Adenovirus vector. The packaged adenovirus can be used to transfect your target cells and deliver the miRNA inhibitor into the cells and knockdown its target mature miRNA.
is it possible to use your kit (miRNA cDNA Synthesis Kit) to do quantitative PCR with the Taqman strategy?
It is definitely possible, but you need to use primers from ABM and design your own TaqMan probe according to your miRNA target.