Adenovirus Expression Systems

Recombinant adenovirus is one of the most efficient gene delivery vehicles for both in vitro and in vivo protein expression due to the high infection efficiency and subsequent yield of recombinant protein. Adenoviruses can infect a broad range of cells, including dividing and non-dividing cells, stem cells and primary cells, and are particularly useful for infecting cell lines that have low transfection efficiency with liposome based methods.

Adenoviruses do not integrate their genome into the host system and therefore do not risk activating or inactivating host genes. Expression of the recombinant protein of interest is detectable within 24 hours post infection and is transient in nature. The expression will persist as long as the viral genome is not degraded, which is around 1-2 weeks depending on the cell type. Longer expression can be observed in slow dividing cells such as neurons.

As a world leader in adenoviral technology, abm has developed a variety of different adenoviral expression systems which can be used to temporarily alter expression within a sample of cells or to moniter gene expression using reporter genes. These systems allow other scientists to produce their own adenoviruses.

Key features:

  • Biosafety: Replication-incompetent (-E1/-E3) human adenovirus type 5 (Ad5).
  • Almost 100% gene delivery in most cell types; very few cell lines cannot be infected.
  • Can be used for in vivo or in vitro recombinant protein expression.
  • Non-integrating; No alteration to host genome.

Product Information

  • Adenovirus Expression Kits
    Adenovirus Expression Kits
    Click to view the full summary of our Adenovirus Expression System Kits. Available with various tags/reporters, for expressing siRNA, and in a promoterless format.
  • Supplemental Adenovirus Expression kits
    Supplemental Adenovirus Expression kits
    Click here to see our additional materials for adenovirus expression, conveniently available in kit format or as individual components.
  • Titer collection of up to 10^12 pfu/ml
    Titers of up to 1012 pfu/ml available
    View our Custom Adenovirus Services for more options for cloning and packaging.