|Phone:||604-247-2416 (Local), 1-866-757-2414 (Toll free)|
|Payment:||or Purchase Order Number|
|More info:||Additional Information Regarding Orders|
|Our Basic Package (C208) Includes||Deliverables||Lead Time|
||Sequence verified knockouts (at least 1 clone, 2 vials per clone)**, microbial/sterility tested with a service report.||12-16 weeks^^|
|Add-On Services^||Quantity||Cat. No.||Price|
|Cell Line of Your Choice for Gene Editing#||1 Cell Line||C141||$800.00|
|WT Control Cell Line Expressing Cas9 for Comparison
(50% discount for academic customers if ordered with the basic package)
|1 Cell Line||C142||$1,590.00|
|Additional Clones||1 Clone||C143||$250.00|
|Additional Vials of Delivered Clones||1 Vial||C144||$80.00|
|Validation Service by Western Blot (Up to 10 Clones)##||1 Service||C145||$975.00|
|Off-Target Analysis by Whole Genome Sequencing||Per Clone||C146||$4,000.00|
|Additional rounds of selection and screening by Sanger Sequencing||1 Screening||C147||$835.00|
|STR Profiling of WT and Knock-out cells
(50% discount if ordered with the basic package)
If your cell line already contains an antibiotic resistance marker, please specify. Our vectors have puromycin resistance by default.
If your cells require medium other than DMEM and RPMI, we will also require 1L of the specified medium and any applicable growth factors or supplements to be provided by you for the project. Please submit all components of the complete media (e.g. if growth factors, cytokines etc. are applicable) individually to eliminate potential degradation of components in the media during transit. Kindly include instructions for making the complete media in your shipment. Additionally, please provide at least 5 x coated 6-well plates and 10 x T25 flasks if the cells require specially treated culture vessels. Once you have shipped your cells, please forward us the tracking number for custom clearance. Information on how to ship cell samples to abm can be found on our support page: https://www.abmgood.com/Technical-Support.html
Please place an order first prior to submitting your samples. All samples received must have the order confirmation number indicated. Any samples received without this piece of information will be disposed off immediately upon receipt to ensure that all customer information is held in strict confidence.
- at least 1 clone, 2 vials per clone
- Sanger sequencing data showing that there are frameshift mutations in each clone
Off-target analysis: This will need to be done by NGS, whole genome sequencing or exome sequencing, which would require comparing the control (before) sample to the genome edited sample (after). If this is a desired option, we can provide a quote for it, but it will likely be at least $2200 for analyzing 1-2 clones by Exome Seq, and higher for whole genome sequencing.