CRISPR is the most versatile technology for genome editing. abm's offers a variety of vectors and viruses for the expression of Cas9 and its variants. Pair these with sgRNA expression vectors or viruses from our human, mouse and rat Genome-Wide sgRNA Libraries or a custom sgRNA. For CRISPR Knock In a repair template is also needed.

NEW CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are emerging technologies for modulation of gene activity. These technologies use a catalytically inactive Cas9 (dCas9) fused to an effector domain. The dCas9 portion is targeted to a specific promoter using an sgRNA; the effector domain then acts to induce (dCas9-SAM) or repress (dCas9-KRAB) the expression of that gene. For dCas9-SAM the sgRNA targets upstream of the promoter can be selected from our CRISPR Activation Genome-Wide Library or ordered as a custom sgRNA. For dCas9-KRAB the sgRNA targets immediately downstream of the transcription start site, the sgRNA can be ordered as a custom sgRNA.

To learn more about CRISPR please visit our CRISPR Knowledge Base.

To order products, check the corresponding boxes on the right and then click on add to cart.

Cas9 Lentiviral Systems
Product	Cat No.	Quantity	Price
Cas9 Nuclease Lentiviral Vector	K002	10 µg	$85.00
Cas9 Nickase Lentiviral Vector	K005	10 µg	$85.00
Cas9 Double Mutant Lentiviral Vector	K012	10 µg	$395.00
dCas9 C-terminal Cloning Vector	K014	10 µg	$545.00
dCas9 Synergistic Activation Mediator Lentivector	K015	10 µg	$195.00
Cumate-Cas9-2A-Puro Lentiviral Vector	K017	10 µg	$295.00
Cumate-Cas9-2A-Neo Lentiviral Vector	K022	10 µg	$295.00
Cumate-Cas9-SV40-Puro Lentiviral Vector	K024	10 µg	$295.00
dCas9-KRAB Lentiviral Vector	K203	10 µg	$145.00
Cas9 Nuclease Lentivirus	K003	300 µl, 10⁷ IU/ml	$295.00
Cas9 Nickase Lentivirus	K006	300 µl, 10⁷ IU/ml	$295.00
Cas9 Double Mutant Lentivirus	K013	300 µl, 10⁷ IU/ml	$695.00
dCas9 Synergistic Activation Mediator Lentivirus	K016	300 µl, 10⁷ IU/ml	$645.00
Cumate-Cas9-2A-Puro Lentivirus	K021	300 µl, 10⁷ IU/ml	$595.00
Cumate-Cas9-2A-Neo Lentivirus	K023	300 µl, 10⁷ IU/ml	$595.00
Cumate-Cas9-SV40-Puro Lentivirus	K025	300 µl, 10⁷ IU/ml	$595.00
dCas9-KRAB Lentivirus	K204	300 µl, 10⁷ IU/ml	$550.00

Cas9 AAV Systems
Product	Cat No.	Quantity	Price
saCas9 Nuclease AAV Vector	K207	10 μg	$150.00
saCas9 Nuclease AAV Virus (AAV1)	K208	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV2)	K209	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV3)	K210	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV4)	K211	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV5)	K212	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV6)	K213	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV7)	K214	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV8)	K215	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV9)	K216	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV10)	K217	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00
saCas9 Nuclease AAV Virus (AAV11)	K218	2 x 250 µl, 1 x 10⁹ GC/ml	$350.00

Cas9 Adenoviral Systems
Product	Cat No.	Quantity	Price
Cas9 Nuclease Adenovirus	K004	250 µl, 10⁶ pfu/ml	$295.00
Cas9 Nickase Adenovirus	K007	250 µl, 10⁶ pfu/ml	$295.00

Other Cas9 Related Products
Product	Cat No.	Quantity	Price
CRISPR Genomic Cleavage Detection Kit	G932	25 Reactions	$165.00
Anti-Cas9 Antibody	Y300079	100 μl	$195.00
ViralPlus Transduction Enhancer	G698	1.0 ml	$175.00

Note:abm’s sgRNAs are designed for use with Cas9 Nuclease only. Please inquire for custom sgRNA pairs for use with Cas9 Nickase.

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More about CRISPR
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system allows for highly specific genomic disruption and replacement. This simple and robust system requires co-expression of two distinct components: (1) an exonuclease, Cas9 and (2) a target-specific guide RNA (gRNA). Cas9 expression in the target cell or host is achieved by lentiviral vector transfection, lentivirus transduction, or adenovirus transduction. Cas9 will unwind the DNA duplex and cleave both strands upon recognition of the target sequence by the gRNA. The resulting double stranded break is repaired by the non-homologous end joining (NHEJ) pathway, disrupting the open reading frame of the targeted gene by causing an InDel frameshift and/or premature stop codon.

Homology Directed Repair (HDR) with Cas9 Nuclease
In addition to NHEJ, cells are able to perform DNA repair by Homology Directed Repair (HDR), which can be exploited to introduce nucleotide modifications to genomic DNA. A DNA repair template containing the desired sequence is normally transfected into the cell along with the gRNA/Cas9 and must have a high degree of homology to the sequence immediately upstream and downstream of the double stranded break.

Cas9 Nickase for Homologous Recombination and Specificity
The Cas9 nuclease has been modified into a "nickase" so that one of its catalytic domains is inactive, resulting in a single stranded nick instead of a double stranded break at the target site on the genomic DNA. This modification reduces off-target effects in the event that the guide RNA (gRNA) imperfectly binds to an undesired DNA site because not one, but two gRNAs located in close proximity (<20 nucleotides) on opposite strands of the genomic DNA are required in order to create the desired double stranded break. An undesired single stranded nick in the genomic DNA can be quickly repaired by the Homology Directed Repair (HDR) pathway using the other intact strand as the template.

The Cas9 nickase can also be used to create a single stranded nick in the genomic DNA for introducing nucleotide modifications by homologous recombination if the template DNA containing the desired modification is transfected into the cell along with the gRNA/Cas9.

	Guide RNA requirement	InDels induced?	HDR induced?	Recommended Application
Cas9 Nuclease	One gRNA for each target	Yes, most efficient	Yes	InDel, HDR (when accuracy, i.e. off-target effects, is not a big concern)
Cas9 Nickase	One gRNA for each target	Barely detectable	Yes, less efficient	HDR without InDel generation
Cas9 Nickase	Two gRNAs for each target	Yes, very efficient	Yes	InDel, HDR (when accuracy, i.e. off-target effects, is a concern)